Microglia Differentiation Service Details
- Standard
- Fully Customizable Service - Inquire
Standard Packaging:
- Ship 1-2 million iPS cells (1-2 cryovials; 1 million cells/vial); OR
- With pathogen and SARS-CoV-2 test results
- Optional Pathogen Testing Service is available
- With pathogen and SARS-CoV-2 test results
- Select one of our Control iPS Cell Lines; OR
- Let ASC generate your iPSCs from your human or non-human samples
Standard Deliverables:
- Cryopreserved iPSC-derived microglia
- Antibody staining images and/or flow cytometry data for the cell type-specific markers
- IBA1, TMEM119, P2RY12, CX3CR1, TREM2; other markers available upon request
- Recovery Test, Mycoplasma Test
- Sub-culture protocol and media (optional)
- Other markers (available upon request)
Standard Workflow and Timeline:
Timeline = 2 months
Optional! RT-PCR, Western Blot, proliferation assays, phagocytosis, functional assay
Applications:
- Neurodevelopment and neuroinflammation research
- Disease modeling for neuroinflammation and neurodegenerative diseases
- Mimic in vivo environment by co-culture with iPSC-derived neurons and glial cells for cell-cell interaction studies and signaling pathway
- In vitro and in vivo (adoptive transfer) cell line model to investigate microglial dysfunction in CNS disorders
- High throughput drug target discovery, efficacy, and toxicity testing