Research

Accelerate your research and discoveries by leveraging our extensive expertise in stem cell technology! We can provide physiologically relevant in vitro models derived from Pluripotent Stem Cells (PSC) for more predictive models of human disease and biology. ASC offers a comprehensive custom service platform for every aspect of stem cell-based research: iPSC generation/ ESC derivation, CRISPR/Cas9 genome editing and cell line model generation; differentiation to somatic lineage of choice, downstream assays to validate your cell line and for drug discovery and screening.

Applied StemCell has a comprehensive catalog of products for every aspect of your stem cell research:

• Ready-to-use induced pluripotent stem cell (iPSC).
• iPSC-derived differentiated neural lineage cells.
• For the more hands-on researcher, we offer protocols and kits for iPSC generation to generate your own iPSCs.
• To help you maintain your stem cells, we also offer high-quality and thoroughly tested mouse embryonic fibroblasts (MEF) feeder cells, and stem cell-grade “pre-validated“ FBS.
• GMP iPSCs >> Learn More

GMP iPSC Products & Services >> Learn More

ASC is a leading provider of genetically engineered mouse models for biomedical research and preclinical drug discovery. With 13+ years of expertise in mouse model engineering and >500 engineered mouse models under our belt, we can engineer advanced, physiologically relevant mouse models with a wide range of precision genetic modifications specific to your projects’ needs.

• Gene knockout (and conditional knockout), small fragment knock-in (e.g., Rosa26 knock-in & reporter mice), and point mutation (SNP)
• F1 breeding for germline transmission
• Very efficient and cost-effective protocols
• FREE design consultation with our experts
• We can help you develop a gene-editing strategy
• Full characterization services

We also offer a repository of off-shelf mouse models that can be directly used for studies on gene function, drug screening, and human diseases, thus effectively shortening your overall experimental cycle.

Applied StemCell, a long-standing leader in custom mouse model engineering, also offers 2,000+ “off-shelf” proprietary, genetically engineered mouse models (GEMM) that are research-ready and can be directly used in gene function, drug screening, and human disease research.

• Humanized immune checkpoint models with single, double or triple-target modifications in PD1, PD-L1, CTLA4, and other genes
• Cytokine receptor-related models with fluorescent tags for live-imaging studies.
• Immunodeficient models for immune system and cancer studies.
• Human disease models with knockout, knock-in, conditional knockout mutations.
• Cre recombinase, reporter genes, Cas9-expressing models, and more.
• >30 fluorescent-labeled tumor cell lines for use in in vitro experiments and tumor studies.

Genetically engineered rat models are gaining popularity as the preferred biological model for several research areas. ASC can genetically engineer rat models with physiologically relevant modifications using an expanded technology portfolio with CRISPR/Cas9 and traditional homologous recombination and random transgenic technologies.

• High-quality service
• F1 breeding for germline transmission
• New! surgically/ chemically induced rat models of neurological diseases
• Customized projects for in vivo assessments (automated behavior/ locomotor activity, EEG/ ECG, and pharmacokinetics) as well as in vitro evaluations (electrophysiology, immunohistochemistry & other biochemical assays)

Applied StemCell’s proprietary site-specific, integrase-based TARGATT™ technology has been used to generate stable, high-quality TARGATT™ Master iPS, CHO, and HEK293 cell lines for large transgenes knock-in. However, if you would like to knock in your gene-of-interest (GOI) into your own cell line, our experts can engineer the TARGATT™ landing pad into your cells. With your engineered TARGATT™ cell line and our unique integrase, you can insert a single copy of your DNA fragment (up to 20kb) at a safe harbor locus. If you would like to learn more, contact us today to schedule your free consultation.

• Fully customizable service
• Our experts can help enhance your project design
• Generate your TARGATT™"Master" cells for reporter/tag line development,  conditional gene expression models, and more
• Free consultation
• Fast turn around
• Off-the-shelf TARGATT™ Master iPS, CHO, and HEK293 Cell Lines are available
• TARGATT™ Advantages
  • Single copy knockin: 1 cell, 1 docking site, 1 inserted transgene
  • Site-specific knockin into a high expression, safe harbor locus (H11, ASC2)
  • High-efficiency integration
  • Large cell library construction in HEK293/CHO cells
  • Cost-effective: no virus packaging time and resources

As a long-standing leader in genetically engineered animal models, Applied StemCell also offers a comprehensive custom research solutions platform for downstream assays in mouse and rat models. Our state-of-the-art vivarium is equipped with automated cages & devices for behavioral assessments, sample collection and in vivo measurements (ECG, EEG) of the animals. We have a multidisciplinary team of expert scientists who can design a comprehensive project plan to fit any requirement/stage of your research pipeline and for drug discovery and screening.

• Designing and engineering research animal disease models
• Adoptive transfers/transplantation
• In vivo functional screening assays
• End-of-study in vitro assays

Cell based assays have become an integral part of preclinical studies to elucidate biological mechanisms, and for screening drugs for efficacy, toxicity or combination analysis for drug synergy or antagonism. The use of live cells in these assays provide a fast and biologically relevant source of information. Applied StemCell offers a fully comprehensive panel of Cell-Based Assays from cell line expansion, cell line model generation, validation, to assays that evaluate cell line function and behavior. We have extensive experience in culturing and handling a wide variety of cell lines including cancer cell lines, immortalized cell lines, stem cells and differentiated cell lines. We will work with you at every stage of your project to fit it to the exact needs of your research.

A comprehensive custom Lentiviral-based Stable Cell Line Generation Service for genetically engineering sensitive cells, primary cells, stem cells and terminally differentiated cells. Lentivirus-based stable cell line generation offers a cost-effective and efficient alternative for generating stable cell line models for gene overexpression, inducible gene expression, gene knockout and gene knockdown.

Key Features:

• Modified lentiviruses with broad tropism to target most mammalian species and cell types.
• Non-integrating lentiviruses for CRISPR-lenti gene knockout.
• Choice of selection markers: antibiotic resistance or GFP for FACS.
• Use 3rd generation lentiviral systems for the highest biosafety standards.
• Gene editing confirmed by NGS or RT-PCR.

With >10 years’ experience in molecular biology and genome editing technologies, ASC offers start-to-finish customized services for DNA cloning. vector design and lentivirus/ retrovirus packaging. We can develop the best strategies to tackle your cloning projects and technical problems.

• Lenti-/ retrovirus packaging: Transduction-ready, high titer viruses packaged with your targeting vectors (CRISPR vectors, CAR-T expression vectors)
• CRISPR vector design and cloning for cell line and animal model generation using benchmark strategies and optimized protocols.
• Vector designing or cloning for RNAi/ inducible vectors homologous recombination, Bacterial Artificial Chromosome (BAC) Recombineering, de novo gene synthesis, site-specific mutagenesis, gene tagging, and more.

Applied StemCell offers gamma-irradiation service for irradiating cell lines (patient-derived, pooled, genome edited/ corrected cell lines) using a Nuclear Regulatory Commission (NRC)-approved Gamma-emission source to irradiate cells at a constant dose rate and to any desired extent of radiation. Irradiated cell line models are novel toolkits for applications such as generation of co-culture models using non-dividing/ feeder cells; to study mechanisms underlying cancer progression; wound healing and inflammatory responses after irradiation; screen drugs for enhancing irradiation sensitivity of tumor cells; and for xenograft tumor models.

Our Cell Line Irradiation Service includes:

1. Cell line expansion/ harvesting
2. Cell line Irradiation (customized extent of irradiation)
3. Cryopreservation

Primary cells are isolated from tissues, including blood and bone marrow. The isolated human primary cells have the capacity to function similarly to cells in vivo under the right conditions, and they can be incorporated into various areas of research as model systems. Primary cells can be used for drug discovery, toxicity testing, gene therapy, cancer research, and more.

In order to address the needs of the research community, Applied StemCell (ASC) now offers high-quality fresh and frozen human primary cells, including peripheral blood cells, cord blood cells, and bone marrow cells. ASC also provides hematopoietic stem cells, progenitor cells, and immune cells. For fresh cells, we offer on-demand, customized sample collection and processing services. All donors are IRB-approved and have been tested for Syphilis, Hepatitis B (HBV), Hepatitis C (HCV), HTLV-I/II, HIV-1, and HIV-2. Same-day delivery is available for Bay Area customers. Overnight delivery and international shipping are available for all other customers. Contact us today to learn more.

Applied StemCell (ASC) now offers human hematologic tissue products, including human healthy whole/peripheral blood and cord blood samples. Plasma, Leukopak, and Off-the-clot serum products are available. ASC offers on-site procurement and processing. Our samples are collected from IRB-approved donors who have tested negative for Syphilis, Hepatitis B (HBV), Hepatitis C (HCV), HTLV-I/II, HIV-1, and HIV-2. Contact ASC today to learn more about customized sample collection and requesting a specific donor.

Non-human primate (NHP) cells and hematologic products are now available. Our non-human primate samples are collected from healthy cynomolgus macaques or rhesus macaques that are housed in the United States. All of our products are from NHPs that have tested negative for SIV, STLV, SRV, and Herpes B. These high-quality NHP products can be used in disease modeling, toxicity testing, or other areas of research. You can schedule a free consultation today to discuss specific project requirements with one of our experts. You can request an NHP donor of a specific gender or age, and you can reserve the cynomolgus or rhesus donor for the entire duration of your project. Contact us today to learn more.

Applied StemCell (ASC) works closely with clinicians to obtain disease samples from patients who have been recently diagnosed or have not received treatment. ASC provides a diverse selection of human fresh and frozen diseased products that can be used for disease modeling, drug development, and other areas of research. Call us today to place your order. We will have the sample ready and, in your hands, the same day of collection when you order one week in advance. ASC offers same-day delivery to Bay Area customers, and overnight and international shipping is available to all customers. The availability of the diseased products is dependent on the prevalence of the disease. If you would like more information, contact us today!

Applied StemCell (ASC) now offers high purity cytokines and growth factors produced by HEK293T cells. We use novel technology to provide customers with high-purity human cytokines that have not been tagged during production. Additionally, our extensive purification methods allow us to generate human cytokines that exhibit super-low levels of endotoxins. ASC’s high purity and high activity cytokines allow you to avoid the higher levels of endotoxin contamination of cytokines from bacteria and other systems. Contact ASC today to speak with one of our experts to learn more about our active human-specific cytokines and which product best fits your project needs.

If you are looking to expedite your project timeline and you do not require customized FFPE slides, scrolls, or blocks, explore our new collection of FFPE cell pellets and tissues to discover the high-quality FFPE product that can help you drive your research forward. Applied StemCell provides FFPE human and animal cell pellets in various formats, including slides, blocks, and scrolls. Our technology enables the production of round, homogenous pellets that are evenly distributed throughout each section. Although we supply a diverse set of cell pellets with consistent densities, FFPE human and animal tissue products are also available for purchase. When you find what you are looking for, contact us and we will help you place your order.

• Slides, blocks & scrolls
• Cell Pellets & Tissue Samples 
• Round, homogenous cell pellets slides and arrays
• Affordable off-the-shelf products with the option to create your own customized FFPE slide, block, scroll, or array with our cost-effective service platform 

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Cell Lines

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Applied StemCell can isolate primary cells from fresh tissues, including from biopsy samples.

Whether you are looking for formalin fixed paraffin embedded (FFPE) blocks, slides, scrolls, or arrays, we can generate the preserved human or animal product you need for your basic research, disease studies, or drug development project with our collection of customizable cell pellet and tissue FFPE services. Unlike other providers, our unique protocols enable the creation of round cell pellets with a consistent density in every section. If you are looking to preserve a specific animal tissue, our scientists can not only tailor your array to your core size and orientation requirements, our team can combine your cell pellet and tissue samples into a single array. Furthermore, you have the power to further personalize your service by selecting a different cell pellet shape (square, rectangle, etc.) and density (1-90%) or tissue thickness and length. If you would like to learn more, visit our service pages or contact us to schedule your free consultation.

ASC Service Advantages

• Customizable: size (2, 3, 7, 10 mm), shape (circle, rectangle, etc.) density (1-90%), mixing & array (core size, orientation, etc.) options
• Several formats to choose from: single pellet slides, pellet array slides, on-slide control arrays, scroll
• Alternative tissue “stick” options for researchers with limited samples
• Fast & Affordable

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Service Options

TARGATT™ technology enables the site-specific integration of any gene-of-interest (GOI; up to 20kb) at a preselected safe harbor locus with the use of a unique integrase. Applied StemCell has generated many TARGATT™ cell lines, including the TARGATT™ CHO and HEK293 Master Cell Lines. These high-quality cells are now part of ready-to-use TARGATT™ knock-in kits that you can use to insert your GOI in your own lab. We have observed steady, medium to high levels of protein expression when using our master cell lines for customer projects, so you can confidently purchase any kit that best suits your research needs. Additionally, our experts have used engineered TARGATT™ cells to address common bioproduction issues.

The traditional CHO antibody and animal bioproduction methods are inefficient because they require random gene insertion and forced amplification of transgenes. Applied StemCell has addressed these problems by utilizing our expertise in cell line and animal model engineering with our proprietary TARGATT™ technology to develop two bioproduction methods: TARGATT™ CHO Master Cell Line and Transgenic TARGATT™ Rabbits. Both methods offer low cost, efficiency, consistent protein expression and high protein yield that is easily scalable for large-scale bioproduction.

Advantages of the TARGATT™ Master Cell Line:

• High knock-in efficiency
• Site-specific integration into the H11 genomic hotspot well-defined safe harbor locus
• Single gene knockin: one variant - one locus - one cell line
• Unidirectional integration for stable knock-in cell lines
• Uniform, high-level gene expression
• Overcomes challenges such as random insertion, gene silencing, multiple copy gene integration, ablated gene expression.
• TARGATT™ CHO and HEK293 Master Cell Lines
• Transgenic TARGATT™ Rabbits for Bioproduction

If you would like to work with your own cell line, our experts can engineer the TARGATT™ system into your cell line. To learn more, explore our Custom TARGATT™ Master Cell Line Generation page or contact us today to schedule your free consultation.

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Explore ASC’s series of antibody services and discover how we can help you propel your research forward. Whether you are looking to generate monoclonal antibodies or construct a cell-based library for screening, we have several well-established protocols and fully customizable service options for you to select from. Our single B cell antibody discovery platform is great for those seeking high-affinity rabbit monoclonal antibodies while our TARGATT™ HEK293 and CHO cell lines are ideal tools for researchers aiming to establish stable knock-in cell lines or large cell libraries. Visit our service pages or contact us today to learn more.

Advantages of the TARGATT™ Master Cell Lines

• High knock-in efficiency
• Site-specific integration into the H11 genomic hotspot well-defined safe harbor locus
• Single gene knockin: one variant - one locus - one cell line
• Unidirectional integration for stable knock-in cell lines
• HEK293 and CHO cells for library construction

Advantages of ASC's Single B Cell Antibody Discovery Platform

• Unique animal strains/lines (rabbit & alpaca)
• Accelerated and Classical immunization
• Amplification of Single B Cell mRNA Efficient Mammalian Cell Antibody Expression

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