Research

  • Services

    • Stem Cell Services


      Accelerate your research and discoveries by leveraging our extensive expertise in stem cell technology! We can provide physiologically relevant in vitro models derived from Pluripotent Stem Cells (PSC) for more predictive models of human disease and biology. ASC offers a comprehensive custom service platform for every aspect of stem cell-based research: iPSC generation/ ESC derivation, CRISPR/Cas9 genome editing and cell line model generation; differentiation to somatic lineage of choice, downstream assays to validate your cell line and for drug discovery and screening.

      Stem Cell Services Categories

      Faster, High-throughput
      iPSC Genome Editing

      Fast, accurate and efficient CRISPR editing in patient iPSCs, with single cell cloning and custom deliverables for predictive disease modeling.

      Faster, High-throughput
      iPSC Genome Editing

      iPSCs from Patient Samples & Allogenic iPSCs

      Reprogramming iPSC from healthy/disease PBMCs or fibroblasts; Generating allogenic/ immunocompatible iPSCs.

      iPSCs from Patient Samples & Allogenic iPSCs

      iPSC Characterization
      Service

      Services to identify & characterize Pluripotent Stem Cells (iPSC/ ESC): Karyotyping, Pluripotency Marker Assays, qPCR, RNA-seq, STR analysis & more.

      iPSC Characterization
      Service

      Microglia & Neural Stem Cell
      Differentiation

      Service for ESC / iPSC differentiation into Microglia, Neural Stem Cells (NSCs), Motor Neurons and Glial Cells (Astrocytes and Oligodendrocytes).

      Microglia & Neural Stem Cell
      Differentiation

      T Cell, NK Cell
      Differentiation

      iPSC differentiation to T cell, NK cell, dendritic cells, monocytes and other immune cells from healthy/diseased/genome edited iPSCs.

      T Cell, NK Cell
      Differentiation

      Hepatocytes
      Differentiation

      Custom differentiation of iPSCs to mature and robust population of ready-to-use hepatocytes.

      Hepatocytes
      Differentiation

      Cardiomyocytes
      Differentiation

      Comprehensive service to differentiate iPSCs into high purity, functional cardiomyocytes from healthy/ disease samples.

      Cardiomyocytes
      Differentiation

      Retinal Pigment Epithelium
      (RPE) Differentiation

      iPSC differentiation to retinal pigment epithelium (RPE) cells from healthy/diseased/genome edited iPSCs.

      Retinal Pigment Epithelium
      (RPE) Differentiation

      iPSC Culture
      Services

      We offer standard and customized services for culture expansion, scale-up, and 3D culture morphology testing of iPSC and differentiated cells.

      iPSC Culture
      Services

    • Neurotoxicity Screening


      Cell-based in vitro assays for drug toxicity are becoming crucial tools to screen new drug candidates because they are inexpensive, efficient, and ethically compatible preliminary screening alternatives to the more expensive animal testing models.

      Applied StemCell offers a one-stop shopping solution for neurological compound screening. Starting with the engineering of iPSCs from healthy and diseased patient samples; developing disease models via gene editing for drug target discovery and efficacy testing; differentiation into cell lineage(s); cell line model characterization; and ending with a comprehensive panel of cell-based tests for drug efficacy, neurotoxicity, and target discovery that are regulatory compliant.

    • CRISPR/Cas9 Genome Editing


      Save Your Time, Money & Effort! Leverage our extensive expertise in CRISPR/Cas9 genome editing technologies to generate genetically modified mouse, rat, cancer and stem cell lines, with a variety of modifications in a targeted gene of interest. As one of the earliest licensees of CRISPR/Cas9 technology, we have genetically engineered > 1800 unique cell line and animal models for disease modeling, functional genomics, target identification, antibody validation, and validation for drug discovery and screening, and more. We offer affordable, comprehensive custom service with a fast turnaround time to meet the exact requirement of your projects. You can also combine it with our downstream custom assay services for a seamless project workflow.

      CRISPR/Cas9 Genome Editing Categories

      Cell Line Models

      Custom stable cell line model generation using CRISPR/Cas9 for disease modeling, antibody validation, and drug screening.

      Cell Line Models

      Mouse Models

      Custom CRISPR/Cas9 mouse engineering with a variety of genetic modifications: knockout, conditional knockout, point mutation & reporter/gene knock-in.

      Mouse Models

      Rat Models

      Custom CRISPR/Cas9 rat engineering with a variety of genetic modifications: knockout, conditional knockout, point mutation & reporter/gene knock-in.

      Rat Models

    • TARGATT™ Genome Editing


      Applied StemCell’s (ASC) proprietary TARGATT™ technology, enables fast and site-specific, stable integration of large DNA fragments (up to 22 kb) into an intergenic, transcriptionally active safe harbor locus with very high efficiency. The preselected locus is engineered to contain an "attP" integrase recognition landing pad where single-copy gene integration occurs when used in conjunction with an “attB” containing donor plasmid and integrase expression.

      The TARGATT™ gene editing platform is versatile and can be used for the development of large fragment knock-in cell line and animal models, bioproduction, and library construction. This technology circumvents problems associated with random integration such as position effect, and gene silencing or instability due to integration of multiple copies of the transgene.

      Advantages of TARGATT™ Technology:

      • Site-specific knock-in at a pre-selected safe harbor locus (no random integration)
      • Single copy gene insertion
      • Large transgene knock-in (up to 22 kb)
      • No internal genes are interrupted
      • Uninformed expression
      • High integration efficiency & High-level expression of the transgene
      • Eliminates position effect

      ASC can accurately and efficiently engineer the necessary landing pad into the cell line or animal model of your choice. We provide ready-to-use TARGATT TM Master Cell Lines (iPSC, HEK293, and CHO) for the integration of your gene of interest (GOI) at a preselected locus that has been tested for uniformed, high gene expression. ASC even supplies an assortment of off-the-shelf TARGATTTM mouse and rat models that you can use in your gene function, drug screening, and human disease research. To learn more about TARGATTTM and how you can incorporate the technology into your research, contact us today!

      TARGATT™ Platforms

      TARGATT™ Master iPSCs for Safe Harbor Knock-in

      TARGATT™ iPSCs for Safe Harbor Knock-in

      - High-efficiency, unidirectional integration
      - Site-specific, single-copy gene knock-in
      - Well-characterized Master Cell Line

      TARGATT™ iPSCs for Safe Harbor knock-in

      TARGATT™ CHO Master Cell lines

      TARGATT™ CHO Master Cell Lines

      - Site-specific gene insertion
      - H11 & ASC2 (A2) safe harbor, transcriptionally active genomic hotspots
      -Ideal for bioproduction

      CTARGATT™ CHO Master Cell Lines

      TARGATT™ HEK293 Master Cell Lines

      TARGATT™ HEK293 Master Cell Lines

      - High knock-in efficiency
      - Site-specific, single-copy gene insertion
      - Overcomes challenges posed by random integration

      TARGATT™ HEK293 Master Cell Lines

       TARGATT™ Site-Specific Knock-in Cell Line Service

      TARGATT™ Site-Specific Knock-in Cell Line Generation Service

      - You send us your favorite cell line
      - We will insert the attP docking site at a specific loci
      - Fast turnaround time: get your cell line in as little as 3 months

      TARGATT™ Site-Specific Knock-in Cell Line Generation Service

      TARGATT™ iPSC-iNK Platform

      TARGATT™ iPSC-iNK Platform

      - >40% gene integration efficiency
      - Site-specific knock-in
      - Transfection by lipofectamine, eliminating viral manufacturing

      TARGATT™ iPSC-iNK Platform

      TARGATT™ Knock-in Mouse Models

      TARGATT™ Knock-in Mouse Models

      - High-efficiency integration (up to 65%)
      - Site-specific integration
      - Germline transmitted F! mice in 5-8 months

      TARGATT™ Knock-in Mouse Models

       


      Technology Comparision

      Comparing TARGATT™ and existing gene editing technologies for stable knock-in cell line development:

      TARGATT™ - For Knock-in Cell Lines

      Contact Us - TARGATT™ Services

    • Bioproduction


      The traditional CHO antibody production and animal bioproduction methods are inefficient because they require random gene insertion and forced amplification of transgenes. Applied StemCell has addressed these problems by utilizing our expertise in cell line and animal model engineering with our proprietary TARGATT™ technology to develop two bioproduction methods: TARGATT™ CHO Master Cell Lines and Transgenic TARGATT™ Rabbits. Both methods offer low-cost, efficiency, consistent protein expression and high protein yield that is easily scalable for large scale bioproduction.

      Bioproduction Categories

      CHO Cells

      Site-specific transgene knock-in using TARGATT CHO Master Cell Lines for the creation of high protein/antibody expression in bioproduction cells.

      CHO Cells

      Transgenic Animals - Rabbit

      Applied StemCell's animal bioproduction platform uses transgenic TARGATT™ Rabbits to consistently express recombinant proteins at a very high yield.

      Transgenic Animals - Rabbit

    • Lentivirus Stable Cell Line Generation


      A comprehensive custom Lentiviral-based Stable Cell Line Generation Service for genetically engineering sensitive cells, primary cells, stem cells and terminally differentiated cells. Lentivirus-based stable cell line generation offers a cost-effective and efficient alternative for generating stable cell line models for gene overexpression, inducible gene expression, gene knockout and gene knockdown.

      Key Features:

      • Modified lentiviruses with broad tropism to target most mammalian species and cell types.
      • Non-integrating lentiviruses for CRISPR-lenti gene knockout.
      • Choice of selection markers: antibiotic resistance or GFP for FACS.
      • Use 3rd generation lentiviral systems for the highest biosafety standards.
      • Gene editing confirmed by NGS or RT-PCR.
    • Virus Packaging & DNA Cloning


      With >10 years’ experience in molecular biology and genome editing technologies, ASC offers start-to-finish customized services for DNA cloning. vector design and lentivirus/ retrovirus packaging. We can develop the best strategies to tackle your cloning projects and technical problems.

      • Lenti-/ retrovirus packaging: Transduction-ready, high titer viruses packaged with your targeting vectors (CRISPR vectors, CAR-T expression vectors)
      • CRISPR vector design and cloning for cell line and animal model generation using benchmark strategies and optimized protocols.
      • Vector designing or cloning for RNAi/ inducible vectors homologous recombination, Bacterial Artificial Chromosome (BAC) Recombineering, de novo gene synthesis, site-specific mutagenesis, gene tagging, and more.
      Virus Packaging / DNA Cloning Categories

      Virus Packaging (Lentivirus and Retrovirus)

      Convenient Custom Lentivirus and Retrovirus Packaging Service that provides you with ready-to-transduce viral particles containing your construct.

      Virus Packaging (Lentivirus and Retrovirus)

      CRISPR / Cas9 Vector
      Design & Construction

      Full service CRISPR/Cas9 vector design, construction and gRNA validation.

      CRISPR / Cas9 Vector
      Design & Construction

      Vector Design / Cloning
      Service

      Comprehensive cloning services for CRISPR/Cas9, BAC and other genome editing technologies, for in vivo and in vitro applications.

      Vector Design / Cloning
      Service

      Bacterial Artificial Chromosome (BAC) Design
      and Cloning

      Custom services for designing, cloning and validating genetic-sequences of interest into a bacterial artificial chromosome (BAC) vector.

      Bacterial Artificial Chromosome (BAC) Design
      and Cloning

    • CRO Services


      With > 12 years experience in genome editing and stem cell technologies, ASC provides ISO:9001 quality, customizable solution-oriented services for advancing preclinical assay development and drug screening for cell and gene therapy pipelines and bioprocessing/ bioproduction:

      • Preclinical proof-of-concept cell line and animal models
      • IND-enabling preclinical assay development, validation, and testing: potency, safety/toxicity, efficacy
      • Assays include dose ranging, biodistribution and efficacy studies in cell line and animal models for your drug discovery and screening applications
      • Consultation for cell and viral vectors manufacturing, analytical methods for drug release (assay development, validation, testing).

      Customize each part of your project to fit the stage of your research.

      CRO Services Categories

      Discovery: Model
      Generation & Validation

      Reliable, custom cell line and animal models, AAV/ lentiviral vector designing, and validation assays for your early-stage cell/ gene therapy projects.

      Discovery: Model
      Generation & Validation

      IND-enabling: Pharmacodynamics,
      Efficacy Assay Development

      Custom assay development for determining proof-of-concept for your CGT, efficacy, pharmacodynamics, biodistribution, and toxicity.

      IND-enabling: Pharmacodynamics,
      Efficacy Assay Development

      CMC Support: Cell and Gene Therapy Development

      Preclinical and non-clinical CMC support for investigational cell and gene therapy products.

      CMC Support: Cell and Gene Therapy Development

    • GMP Grade iPSC Services & Products


      Applied StemCell (ASC) is in the process of getting GLP and GMP certified and complied with. With its core technologies, CRISPR, TARGATTTM, and iPSC, ASC can produce almost any gene modification including therapeutic gene/reporter gene insertion, point mutation correction, small nucleotide addition, replacement, and deletion.

      ASC can now offer a wide range of GMP clinical-grade induced pluripotent stem cell (iPSC) related services. Applied StemCell’s GMP Grade iPSC line (Cord Blood CD34+, Male) can be engineered to express almost any gene of interest (CRISPR; TARGATTTM), but ASC can also conduct gene-editing services with the iPSC lines provided by customers. GMP grade knockin cell line generation service using the TARGATT™ Master iPSC Line is also available for single-copy gene insertion at a safe harbor locus.

      Along with gene-editing services, ASC offers comprehensive iPSC differentiation service to committed somatic lineages. ASC can work with its GMP iPSC lines or researchers can send in their healthy, diseased, or gene-edited iPSC lines and Applied StemCell will produce the iPSC differentiated cells in its GMP facility.

      ASC is working towards developing a comprehensive inventory of GMP clinical-grade iPSC-related products and is currently building a TARGATTTM Universal Modifiable iPSC platform for therapeutic cell production.

  • Products

    • Stem Cell


      Applied StemCell has a comprehensive catalog of products for every aspect of your stem cell research:

      • Ready-to-use induced pluripotent stem cell (iPSC).
      • iPSC-derived differentiated neural lineage cells.
      • For the more hands-on researcher, we offer protocols and kits for iPSC generation to generate your own iPSCs.
      • To help you maintain your stem cells, we also offer high quality and thoroughly tested mouse embryonic fibroblasts (MEF) feeder cells, and stem cell-grade “pre-validated“ FBS.
      Stem Cell Categories

      iPSC & ESC Lines

      iPSC & ESC Lines

      Ready-to-use, fully characterized human iPSCs from different sources and clinical conditions, iPSC from other mammalian species, and mouse ESC lines.

      iPSC & ESC Lines

      iPSC Differentiated Cells

      We offer fully-characterized iPSC-differentiated progenitors and cell lines: Neural stem cells (NSC), neurons, astrocytes, and cardiomyocytes.

      iPSC Differentiated Cells

      Genome Edited iPSC Lines

      Control iPSC-derived isogenic panels of neuronal gene knockout, lineage-specific reporter knock-in, and safe harbor locus reporter knock-in iPSC lines.

      Genome Edited iPSC Lines

      Stem Cell Culture:
      MEF Feeder Cells, FBS

      Validated, high quality MEF feeders and ESC-Sure™ FBS for robust growth of your stem cells.

      Stem Cell Culture:
      MEF Feeder Cells, ESC-Grade FBS

      iPSC Generation
      Kit

      Reprogram your own iPSCs from your somatic cells using either our Human EZ- iPSC Retroviral or Episomal Generation kits.

      iPSC Generation
      Kit

    • TARGATT™ Genome Editing


      TARGATT™ site-specific knock-in technology enables the integration of transgenes at a preselected locus in the genome, thus overcoming drawbacks of random integration. This technology is adaptable for gene integration in many different species of animals (mice, rats, rabbits, pigs) and cell lines (including HEK293, CHO cells, stem cells and immortalized cell lines).

      Applications:

      • Knock-in models: transgene overexpression, reporter gene
      • Conditional/ inducible expression models
      • Cre-driver mouse lines
      • Antibody discovery & screening
      • Bioproduction
      TARGATT™ Genome Editing Categories

      TARGATT™ Knockin
      Master Cell Line

      TARGATT™ “attP” Master cell lines and knock-in kit for rapid, site-specific transgene knock-in with high efficiency and robust transgene expression.

      TARGATT™ Knockin
      Master Cell Line

      TARGATT™ Mouse Model Generation

      Do-it-yourself kits to generate site-specific transgenic mice in 3 months! We offer TARGATT™ “attP” mice, plasmids, transgenic kits, genotyping kits.

      TARGATT™ Mouse Model Generation 

      TARGATT™ Knockin Cre-Rat Model Generation

      Transgenic Cre-lox rat models that mimic human cardiovascular or neurological diseases.

      TARGATT™ Knockin Cre-Rat Model Generation 

    • Genetically Modified Cell Lines / Isogenic Cell Lines


      Isogenic cell lines represent a unique toolset for studying cellular biology, such as the impact of genotype on cellular phenotype, as well as for parallel, high throughput screening to enable the discovery of therapeutic compounds that exhibit genotype-specific toxicity.  An isogenic cell line is a cell line that has been engineered from a parental line through the introduction of a targeted gene mutation. In doing so, the parental cell line inherently becomes a control line to which the engineered line can be referenced. Applied StemCell provides ready-to-use engineered cell lines for cancer and neurodegenerative research as well as reporter lines for quantitative assessments of drug screening experiments.

      Genetically Modified Cell Lines / Isogenic Cell Lines Categories

      Neuronal Disease Specific Isogenic Knockout iPSCs

      Fully characterized, isogenic neuronal gene knockout iPSCs engineered from control iPSC line (ASE-9109), ideal for neurological disease modeling.

      Neuronal Disease Specific Isogenic Knockout iPSCs

      Reporter iPSC Lines
      (Isogenic)

      Ready-to-use, isogenic panels of knock-in reporter iPSC lines (neuronal lineage & safe harbor locus) generated from control/ master iPSC (ASE-9109).

      Reporter iPSC Lines
      (Isogenic)

  • Animal Models

    • Mouse Models


      ASC is a leading provider of genetically engineered mouse models for biomedical research and preclinical drug discovery. With 11+ years of expertise in mouse model engineering and >500 engineered mouse models under our belt, we can engineer advanced, physiologically relevant mouse models with a wide range of precision genetic modifications specific to your projects’ needs.

      • Gene knockout, point mutation (SNP), small fragment knock-in, large transgene knock-in (locus-specific and safe harbor locus)
      • F1 breeding for germline transmission
      • Very efficient and cost-effective protocols

      We also offer a repository of off-shelf mouse models that can be directly used for studies on gene function, drug screening, and human diseases, thus effectively shortening your overall experimental cycle.

      As an ISO company Applied StemCell can offer you:

      • FREE design consultation with our experts
      • We can help you develop a gene-editing strategy
      • Access to our well characterized control lines
      • Full characterization services
      • Further differentiation capabilities

      Summer Sale! Custom Knockout Mouse Model Generation $10K USD

      Mouse Models Categories

      Conditional Knockout
      Mouse Models

      Conditional knockout/ expression mouse model generation for development and tissue-specific controlled or inducible gene expression.

      Conditional Knockout
      Mouse Models

      Knockout, Knock-in, Point
      Mutation Mouse Models

      Custom mouse model generation with biorelevant and precise genetic modifications: gene knockout, knock-in, point mutation and more.

      Knockout, Knock-in, Point
      Mutation Mouse Models

      Site-specific TARGATT™
      Knock-in Mouse Models

      Fast, Reliable & Site-specific, large transgene knock-in in a safe harbor locus for gene overexpression and custom Cre mouse line generation.

      Site-specific TARGATT™
      Knock-in Mouse Models

    • Mouse Repository


      Inquire!

      Applied StemCell, a long-standing leader in custom mouse model engineering, also offers 2,000+ “off-shelf” proprietary, genetically engineered mouse models (GEMM) that are research-ready and can be directly used in gene function, drug screening, and human disease research.

      • Humanized immune checkpoint models with single, double or triple-target modifications in PD1, PD-L1, CTLA4 and other genes.
      • Cytokine receptor-related models with fluorescent tags for live-imaging studies.
      • Immunodeficient models for immune system and cancer studies.
      • Human disease models with knockout, knock-in, conditional knockout mutations.
      • Cre recombinase, reporter genes, and Cas9-expressing models and more.
      • >30 fluorescent-labeled tumor cell lines for use in in vitro experiments and tumor studies.
      Mouse Repository Categories

      “Immune

      Humanized Immune
      Checkpoint Mouse Models

      Mouse models with single, double & triple humanized immune checkpoint genes (PD-1, PD-L1, CTLA4, CD40 and more) for immuno-oncology efficacy studies.

      Humanized Immune Checkpoint Mouse Models

      Cre Mouse Models

      Cre Mouse Models

      Cre-recombinase expressing mouse lines as a research tool to generate conditional or inducible gene expression/ knockout mouse models.

      Cre Mouse Models

      Cytokine Mouse Models

      Humanized cytokine gene mouse models for immune response studies, inflammatory disease mouse modeling, immunotherapy efficacy and drug screening.

      Cytokine Mouse Models

      Other Gene Edited Mouse Models

      Other Gene Edited Mouse Models

      Off-shelf human disease models featuring knock-in, knock-out, point mutations, cKI/cKO as well as tool mice for advanced mouse model generation.

      Other Gene Edited Mouse Models

      Immunodeficient Mouse Models

      Immunodeficient Mouse Models

      Immunodeficient mouse models (such as M-NSG, Rag1-KO, TCRa and more) for xenografting (PDX, CDX), immuno-oncology, inflammatory & immunology studies.

      Immunodeficient Mouse Models

      Immunodeficient Mouse Models

      Humanized ACE2 Mice for COVID-19 Research

      Coronavirus (SARS-CoV) ACE2 mouse models for viral pathogenesis research and for screening antiviral therapies and vaccines.

      Humanized ACE2 Mice for COVID-19 Research

    • Rat Models


      Genetically engineered rat models are gaining popularity as the preferred biological model for several research areas. ASC can genetically engineer rat models with a physiologically relevant modifications using an expanded technology portfolio with complementary CRISPR/Cas9 and TARGATT™ technologies, as well as, traditional homologous recombination and random transgenic technologies.

      • High-quality service
      • F1 breeding for germline transmission
      • New! surgically/ chemically induced rat models of neurological diseases
      • Customized projects for in vivo assessments (automated behavior/ locomotor activity, EEG/ ECG, and pharmacokinetics) as well as in vitro evaluations (electrophysiology, immunohistochemistry & other biochemical assays)
      Rat Models Categories

      Conditional Knockout
      Rat Models

      Conditional knock-out/ expression mouse model generation to accurately model temporal and spatially controlled gene expression in rats.

      Conditional Knockout
      Rat Models

      Site-Specific TARGATT™
      Knock-in Rat Models

      Fast and Reliable! Site-specific, safe harbor locus large transgene knock-in for gene overexpression and custom Cre rat line generation.

      Site-Specific TARGATT™
      Knock-in Rat Models

      Knockout, Knock-in, Point
      Mutation Rat Models

      Full service CRISPR rat model generation with precise gene knockout, knock-in, and point mutation modifications for basic and preclinical research.

      Knockout, Knock-in, Point
      Mutation Rat Models

      Site-Specific TARGATT™
      Knock-in Cre-Rat Models

      Leverage our neurological or cardiovascular tissue-specific Cre-expressing promoter constructs with your rat to accelerate your research.

      Site-Specific TARGATT™
      Knock-in Cre-Rat Models

       

    • Custom In Vivo Assay Services


      Based on our world-class animal model genetic engineering technologies, Applied StemCell offers a comprehensive suite of downstream assays in mouse and rat models to validate your animal models and for drug screening. We have a multidisciplinary team of expert scientists who can design a comprehensive project plan to fit any requirement/stage of your research pipeline and for drug discovery and screening.

      • Designing and engineering research animal disease models
      • Adoptive transfers/transplantation
      • In vivo functional screening assays
      • End-of-study in vitro assays
      • Antibody efficacy testing studies for immunotherapy for cancer and autoimmune disorders
  • Cell Line Models

    • Stem Cells - iPSC and ESCs


      Applied StemCell’s stem cell division has scientists with >75 years of combined experience in stem cell technology. We provide the most comprehensive stem cell related service and product platform for every stage of your stem cell research. Our stem cell service platform makes use the latest and most optimized protocols for efficient and successful projects. Custom stem cell service encompasses stem cell generation, disease modeling, differentiation, characterization to downstream validation of your models and drug screening assays. Our stem cell product catalog includes a variety of ready-to-use, well-characterized iPSC lines, pre-differentiated isogenic panels of neural lineage cells, MEF feeder cells and stem-cell grade FBS among other ISO:9001 quality products.

    • TARGATT™ HEK293 Master Cell Line for Site-Specific Knockin


      Mammalian Cell-based Protein/ Antibody Discovery & Screening! The TARGATT™ HEK293 Master Cell Line provides an efficient way to generate site-specific, stable, knockin cell lines (even large transgenes) and build large mammalian cell libraries:

      • High knockin efficiency: with enrichment (up to 90%) or without (up to 40%)
      • Site-specific: H11 locus; active, genomic hotspot
      • Single copy integration
      • Uniform, high level gene expression
      • Unidirectional integration
      • Overcomes challenges posed by random integration

      The TARGATT™ HEK293 Master Cell Line is ideal for drug screening, toxicity studies, CNS disease or cell modeling, and regenerative medicine studies.

    • Cell-Based Assays


      Cell based assays have become an integral part of preclinical studies to elucidate biological mechanisms, and for screening drugs for efficacy, toxicity or combination analysis for drug synergy or antagonism. The use of live cells in these assays provide a fast and biologically relevant source of information. Applied StemCell offers a fully comprehensive panel of Cell-Based Assays from cell line expansion, cell line model generation, validation, to assays that evaluate cell line function and behavior. We have extensive experience in culturing and handling a wide variety of cell lines including cancer cell lines, immortalized cell lines, stem cells and differentiated cell lines. We will work with you at every stage of your project to fit it to the exact needs of your research.

    • Cell-Based Drug Toxicity and Efficacy Testing


      Make Informed Go-No-Go Decisions Early in Your Drug Development! Process! Applied StemCell (ASC), an ISO-certified service provider offers a flexible assay platform with a wide-range of functional endpoints for early-stage in vitro screening of preclinical drug candidates. We use a comprehensive cell-based test battery from which you can choose assays for efficacy, safety or target discovery that suit your therapeutic pipeline. We will provide the scientific expertise, accurate and efficient screening to help you take informed decisions about your small/ large molecules early in your drug development process to avoid costly drug attrition. Our custom screening service is ideal for CNS, cardiovascular, metabolic, cancer, and immunotherapy drug candidates.

    • Bioproduction Services- High Yield CHO Cell Lines


      TARGATT™ for Bioproduction of Recombinant Proteins in Chinese Hamster Ovary (CHO) cells. The traditional CHO-cell based protein production is inefficient because it requires random insertion and forced amplification of transgenes.  ASC has “master” TARGATT™ CHO cell lines in two lineages to enable site-specific, single-copy insertion of large transgenes into a preselected safe harbor locus in the CHO cell genome, for high expression levels of your gene of interest. This site-specific TARGATT™ technology overcomes challenges posed by traditional insertion methods, and results in high integration efficiency (>90%), stable knock-in cell lines, uniform gene expression and reproducibility of clones. The Master TARGATT™ CHO cell lines are ideal for antibody and recombinant protein expression.

    • Custom Services


      To speed up your research and development; we provide pre-clinical development services (CRO) and support. Our custom services will allow you to focus on the main aspects of your research while we develop the interim paradigms for you.

      Custom Services Categories

      Fibroblasts Immortalization Service

      Simian virus 40 T antigen to immortalize human fibroblasts is the foundation of Applied StemCell’s Fibroblasts Immortalization Service platform.

      Fibroblasts Immortalization Service

      Custom Primary Cell Isolation Service

      Employing optimal isolation and culturing techniques, Applied StemCell will isolate primary cells from organs or tissues for your ideal cell model.

      Custom Primary Cell Isolation Service

      FFPE Service

      Preserve your cells by FFPE (Formalin-Fixed Paraffin-Embedded) to create a reference standard, use for IHC, ISH or other applications.

      FFPE Service

  • Primary Cells & Tissue Samples

    • Human Primary Cells


      Primary cells are isolated from tissues, including blood and bone marrow. The isolated human primary cells have the capacity to function similarly to cells in vivo under the right conditions, and they can be incorporated into various areas of research as model systems. Primary cells can be used for drug discovery, toxicity testing, gene therapy, cancer research, and more.

      In order to address the needs of the research community, Applied StemCell (ASC) now offers high-quality fresh and frozen human primary cells, including peripheral blood cells, cord blood cells, and bone marrow cells. ASC also provides hematopoietic stem cells, progenitor cells, and immune cells. For fresh cells, we offer on-demand, customized sample collection and processing services. All donors are IRB-approved and have been tested for Syphilis, Hepatitis B (HBV), Hepatitis C (HCV), HTLV-I/II, HIV-1, and HIV-2. Same-day delivery is available for Bay Area customers. Overnight delivery and international shipping are available for all other customers. Contact us today to learn more.

       

      Place Your Order Today!

      Ordering

      • Call 1-866-497-4180
      • Email: info@appliedstemcell.com
      • Contact Us
      • Order Online

      Ordering

      Collection

      • HIPPA & IRB Adherence
      • On-demand, customized sample collection
      • Request a specific donor
      • Customized aliquots 

      Collection

      Processing

      • Completed on-site in the U.S.
      • Our scientists have 20+ years of experience
      • Optimized protocols

      Processing

      Shipping & Delivery

      • Bay Area same-day delivery; Overnight delivery for all other U.S. customers
      • Worldwide shipping

      Shipping & Delivery

    • Human Hematologic Tissue Products


      Applied StemCell (ASC) now offers human hematologic tissue products, including human healthy whole/peripheral blood and cord blood samples. Plasma, Leukopak, and Off-the-clot serum products are available. ASC offers on-site procurement and processing. Our samples are collected from IRB-approved donors who have tested negative for Syphilis, Hepatitis B (HBV), Hepatitis C (HCV), HTLV-I/II, HIV-1, and HIV-2. Contact ASC today to learn more about customized sample collection and requesting a specific donor.

      Place Your Order Today!

      Ordering

      • Call 1-866-497-4180
      • Email: info@appliedstemcell.com
      • Contact Us
      • Order Online

      Ordering

      Collection

      • HIPPA & IRB Adherence
      • On-demand, customized sample collection
      • Request a specific donor
      • Customized aliquots 

      Collection

      Processing

      • Completed on-site in the U.S.
      • Our scientists have 20+ years of experience
      • Optimized protocols

      Processing

      Shipping & Delivery

      • Bay Area same-day delivery; Overnight delivery for all other U.S. customers
      • Worldwide shipping

      Shipping & Delivery

    • Non-Human Primate Cells & Hematologic Tissue Products


      Non-human primate (NHP) cells and hematologic products are now available. Our non-human primate samples are collected from healthy cynomolgus macaques or rhesus macaques that are housed in the United States. All of our products are from NHPs that have tested negative for SIV, STLV, SRV, and Herpes B. These high-quality NHP products can be used in disease modeling, toxicity testing, or other areas of research. You can schedule a free consultation today to discuss specific project requirements with one of our experts. You can request an NHP donor of a specific gender or age, and you can reserve the cynomolgus or rhesus donor for the entire duration of your project. Contact us today to learn more.

      Place Your Order Today!

      Ordering

      • Call 1-866-497-4180
      • Email: info@appliedstemcell.com
      • Contact Us
      • Order Online

      Ordering

      Collection

      • HIPPA & IRB Adherence
      • On-demand, customized sample collection
      • Request a specific donor
      • Customized aliquots 

      Collection

      Processing

      • Completed on-site in the U.S.
      • Our scientists have 20+ years of experience
      • Optimized protocols

      Processing

      Shipping & Delivery

      • Bay Area same-day delivery; Overnight delivery for all other U.S. customers
      • Worldwide shipping

      Shipping & Delivery

    • Human Diseased Products


      Applied StemCell (ASC) works closely with clinicians to obtain disease samples from patients who have been recently diagnosed or have not received treatment. ASC provides a diverse selection of human fresh and frozen diseased products that can be used for disease modeling, drug development, and other areas of research. Call us today to place your order. We will have the sample ready and, in your hands, the same day of collection when you order one week in advance. ASC offers same-day delivery to Bay Area customers, and overnight and international shipping is available to all customers. The availability of the diseased products is dependent on the prevalence of the disease. If you would like more information, contact us today!

      Place Your Order Today!

      Ordering

      • Call 1-866-497-4180
      • Email: info@appliedstemcell.com
      • Contact Us
      • Order Online

      Ordering

      Collection

      • HIPPA & IRB Adherence
      • On-demand, customized sample collection
      • Request a specific donor
      • Customized aliquots 

      Collection

      Processing

      • Completed on-site in the U.S.
      • Our scientists have 20+ years of experience
      • Optimized protocols

      Processing

      Shipping & Delivery

      • Bay Area same-day delivery; Overnight delivery for all other U.S. customers
      • Worldwide shipping

      Shipping & Delivery

    • Cytokines & Growth Factors


      Applied StemCell (ASC) now offers high purity cytokines and growth factors produced by HEK293T cells. We use novel technology to provide customers with high-purity human cytokines that have not been tagged during production. Additionally, our extensive purification methods allow us to generate human cytokines that exhibit super-low levels of endotoxins. ASC’s high purity and high activity cytokines allow you to avoid the higher levels of endotoxin contamination of cytokines from bacteria and other systems. Contact ASC today to speak with one of our experts to learn more about our active human-specific cytokines and which product best fits your project needs.

      Place Your Order Today!

      Ordering

      • Call 1-866-497-4180
      • Email: info@appliedstemcell.com
      • Contact Us
      • Order Online

      Ordering

      Collection

      • HIPPA & IRB Adherence
      • On-demand, customized sample collection
      • Request a specific donor
      • Customized aliquots 

      Collection

      Processing

      • Completed on-site in the U.S.
      • Our scientists have 20+ years of experience
      • Optimized protocols

      Processing

      Shipping & Delivery

      • Bay Area same-day delivery; Overnight delivery for all other U.S. customers
      • Worldwide shipping

      Shipping & Delivery

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!

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