Research

  • Service

    • Stem Cell Services


      Accelerate your research and discoveries by leveraging our extensive expertise in stem cell technology! We can provide physiologically relevant in vitro models derived from Pluripotent Stem Cells (PSC) for more predictive models of human disease and biology. ASC offers a comprehensive custom service platform for every aspect of stem cell-based research: iPSC generation/ ESC derivation, CRISPR/Cas9 genome editing and cell line model generation; differentiation to somatic lineage of choice, downstream assays to validate your cell line and for drug discovery and screening.

      Stem Cell Services Categories

      Faster, High-throughput
      iPSC Genome Editing

      Fast, accurate and efficient CRISPR editing in patient iPSCs, with single cell cloning and custom deliverables for predictive disease modeling.

      Faster, High-throughput
      iPSC Genome Editing

      iPSCs from Patient Samples & Allogenic iPSCs

      Reprogramming iPSC from healthy/disease PBMCs or fibroblasts; Generating allogenic/ immunocompatible iPSCs.

      iPSCs from Patient Samples & Allogenic iPSCs

      iPSC Characterization
      Service

      Services to identify & characterize Pluripotent Stem Cells (iPSC/ ESC): Karyotyping, Pluripotency Marker Assays, qPCR, RNA-seq, STR analysis & more.

      iPSC Characterization
      Service

      Microglia & Neural Stem Cell
      Differentiation

      Service for ESC / iPSC differentiation into Microglia, Neural Stem Cells (NSCs), Motor Neurons and Glial Cells (Astrocytes and Oligodendrocytes).

      Microglia & Neural Stem Cell
      Differentiation

      T Cell, NK Cell
      Differentiation

      iPSC differentiation to T cell, NK cell, dendritic cells, monocytes and other immune cells from healthy/diseased/genome edited iPSCs.

      T Cell, NK Cell
      Differentiation

      Hepatocytes
      Differentiation

      Custom differentiation of iPSCs to mature and robust population of ready-to-use hepatocytes.

      Hepatocytes
      Differentiation

      Cardiomyocytes
      Differentiation

      Comprehensive service to differentiate iPSCs into high purity, functional cardiomyocytes from healthy/ disease samples.

      Cardiomyocytes
      Differentiation

      Retinal Pigment Epithelium
      (RPE) Differentiation

      iPSC differentiation to retinal pigment epithelium (RPE) cells from healthy/diseased/genome edited iPSCs.

      Retinal Pigment Epithelium
      (RPE) Differentiation

      iPSC Culture
      Services

      We offer standard and customized services for culture expansion, scale-up, and 3D culture morphology testing of iPSC and differentiated cells.

      iPSC Culture
      Services

  • Products

    • Stem Cell


      Applied StemCell has a comprehensive catalog of products for every aspect of your stem cell research:

      • Ready-to-use induced pluripotent stem cell (iPSC).
      • iPSC-derived differentiated neural lineage cells.
      • For the more hands-on researcher, we offer protocols and kits for iPSC generation to generate your own iPSCs.
      • To help you maintain your stem cells, we also offer high-quality and thoroughly tested mouse embryonic fibroblasts (MEF) feeder cells, and stem cell-grade “pre-validated“ FBS.
      • GMP iPSCs >> Learn More
      Stem Cell Categories

      iPSC & ESC Lines

      iPSC & ESC Lines

      Ready-to-use, fully characterized human iPSCs from different sources and clinical conditions, iPSC from other mammalian species, and mouse ESC lines.

      iPSC & ESC Lines

      iPSC Differentiated Cells

      We offer fully-characterized iPSC-differentiated progenitors and cell lines: Neural stem cells (NSC), neurons, astrocytes, and cardiomyocytes.

      iPSC Differentiated Cells

      Genome Edited iPSC Lines

      Control iPSC-derived isogenic panels of neuronal gene knockout, lineage-specific reporter knock-in, and safe harbor locus reporter knock-in iPSC lines.

      Genome Edited iPSC Lines

      Stem Cell Culture:
      MEF Feeder Cells, FBS

      Validated, high quality MEF feeders and ESC-Sure™ FBS for robust growth of your stem cells.

      Stem Cell Culture:
      MEF Feeder Cells, ESC-Grade FBS

      iPSC Generation
      Kit

      Reprogram your own iPSCs from your somatic cells using either our Human EZ- iPSC Retroviral or Episomal Generation kits.

      iPSC Generation
      Kit

      GMP iPSC Products & Services >> Learn More

  • Animal Models

    • CRISPR Mouse Models


      ASC is a leading provider of genetically engineered mouse models for biomedical research and preclinical drug discovery. With 13+ years of expertise in mouse model engineering and >500 engineered mouse models under our belt, we can engineer advanced, physiologically relevant mouse models with a wide range of precision genetic modifications specific to your projects’ needs.

      We also offer a repository of off-shelf mouse models that can be directly used for studies on gene function, drug screening, and human diseases, thus effectively shortening your overall experimental cycle.

    • Mouse Repository


      Applied StemCell, a long-standing leader in custom mouse model engineering, also offers 2,000+ “off-shelf” proprietary, genetically engineered mouse models (GEMM) that are research-ready and can be directly used in gene function, drug screening, and human disease research.

      • Humanized immune checkpoint models with single, double or triple-target modifications in PD1, PD-L1, CTLA4, and other genes
      • Cytokine receptor-related models with fluorescent tags for live-imaging studies.
      • Immunodeficient models for immune system and cancer studies.
      • Human disease models with knockout, knock-in, conditional knockout mutations.
      • Cre recombinase, reporter genes, Cas9-expressing models, and more.
      • >30 fluorescent-labeled tumor cell lines for use in in vitro experiments and tumor studies.
      Mouse Repository Categories

      “Immune

      Humanized Immune
      Checkpoint Mouse Models

      Mouse models with single, double & triple humanized immune checkpoint genes (PD-1, PD-L1, CTLA4, CD40 and more) for immuno-oncology efficacy studies.

      Humanized Immune Checkpoint Mouse Models

      Cre Mouse Models

      Cre Mouse Models

      Cre-recombinase expressing mouse lines as a research tool to generate conditional or inducible gene expression/ knockout mouse models.

      Cre Mouse Models

      Cytokine Mouse Models

      Humanized cytokine gene mouse models for immune response studies, inflammatory disease mouse modeling, immunotherapy efficacy and drug screening.

      Cytokine Mouse Models

      Other Gene Edited Mouse Models

      Other Gene Edited Mouse Models

      Off-shelf human disease models featuring knock-in, knock-out, point mutations, cKI/cKO as well as tool mice for advanced mouse model generation.

      Other Gene Edited Mouse Models

      Immunodeficient Mouse Models

      Immunodeficient Mouse Models

      Immunodeficient mouse models (such as M-NSG, Rag1-KO, TCRa and more) for xenografting (PDX, CDX), immuno-oncology, inflammatory & immunology studies.

      Immunodeficient Mouse Models

      Immunodeficient Mouse Models

      Humanized ACE2 Mice for COVID-19 Research

      Coronavirus (SARS-CoV) ACE2 mouse models for viral pathogenesis research and for screening antiviral therapies and vaccines.

      Humanized ACE2 Mice for COVID-19 Research

    • Rat Models


      Genetically engineered rat models are gaining popularity as the preferred biological model for several research areas. ASC can genetically engineer rat models with physiologically relevant modifications using an expanded technology portfolio with CRISPR/Cas9 and traditional homologous recombination and random transgenic technologies.

      • High-quality service
      • F1 breeding for germline transmission
      • New! surgically/ chemically induced rat models of neurological diseases
      • Customized projects for in vivo assessments (automated behavior/ locomotor activity, EEG/ ECG, and pharmacokinetics) as well as in vitro evaluations (electrophysiology, immunohistochemistry & other biochemical assays)
  • Bioservices

    • TARGATT™ Master Cell Line Generation Service


      Applied StemCell’s proprietary site-specific, integrase-based TARGATT™ technology has been used to generate stable, high-quality TARGATT™ Master iPS, CHO, and HEK293 cell lines for large transgenes knock-in. However, if you would like to knock in your gene-of-interest (GOI) into your own cell line, our experts can engineer the TARGATT™ landing pad into your cells. With your engineered TARGATT™ cell line and our unique integrase, you can insert a single copy of your DNA fragment (up to 20kb) at a safe harbor locus. If you would like to learn more, contact us today to schedule your free consultation.

      • Fully customizable service
      • Our experts can help enhance your project design
      • Generate your TARGATT™"Master" cells for reporter/tag line development,  conditional gene expression models, and more
      • Free consultation
      • Fast turn around
      • Off-the-shelf TARGATT™ Master iPS, CHO, and HEK293 Cell Lines are available
      • TARGATT™ Advantages
        • Single copy knockin: 1 cell, 1 docking site, 1 inserted transgene
        • Site-specific knockin into a high expression, safe harbor locus (H11, ASC2)
        • High-efficiency integration
        • Large cell library construction in HEK293/CHO cells
        • Cost-effective: no virus packaging time and resources
    • Custom In Vivo Assay Services


      As a long-standing leader in genetically engineered animal models, Applied StemCell also offers a comprehensive custom research solutions platform for downstream assays in mouse and rat models. Our state-of-the-art vivarium is equipped with automated cages & devices for behavioral assessments, sample collection and in vivo measurements (ECG, EEG) of the animals. We have a multidisciplinary team of expert scientists who can design a comprehensive project plan to fit any requirement/stage of your research pipeline and for drug discovery and screening.

      • Designing and engineering research animal disease models
      • Adoptive transfers/transplantation
      • In vivo functional screening assays
      • End-of-study in vitro assays
    • Cell-Based Assays


      Cell based assays have become an integral part of preclinical studies to elucidate biological mechanisms, and for screening drugs for efficacy, toxicity or combination analysis for drug synergy or antagonism. The use of live cells in these assays provide a fast and biologically relevant source of information. Applied StemCell offers a fully comprehensive panel of Cell-Based Assays from cell line expansion, cell line model generation, validation, to assays that evaluate cell line function and behavior. We have extensive experience in culturing and handling a wide variety of cell lines including cancer cell lines, immortalized cell lines, stem cells and differentiated cell lines. We will work with you at every stage of your project to fit it to the exact needs of your research.

    • Lentivirus Stable Cell Line Generation


      A comprehensive custom Lentiviral-based Stable Cell Line Generation Service for genetically engineering sensitive cells, primary cells, stem cells and terminally differentiated cells. Lentivirus-based stable cell line generation offers a cost-effective and efficient alternative for generating stable cell line models for gene overexpression, inducible gene expression, gene knockout and gene knockdown.

      Key Features:

      • Modified lentiviruses with broad tropism to target most mammalian species and cell types.
      • Non-integrating lentiviruses for CRISPR-lenti gene knockout.
      • Choice of selection markers: antibiotic resistance or GFP for FACS.
      • Use 3rd generation lentiviral systems for the highest biosafety standards.
      • Gene editing confirmed by NGS or RT-PCR.
    • Virus Packaging & DNA Cloning


      With >10 years’ experience in molecular biology and genome editing technologies, ASC offers start-to-finish customized services for DNA cloning. vector design and lentivirus/ retrovirus packaging. We can develop the best strategies to tackle your cloning projects and technical problems.

      • Lenti-/ retrovirus packaging: Transduction-ready, high titer viruses packaged with your targeting vectors (CRISPR vectors, CAR-T expression vectors)
      • CRISPR vector design and cloning for cell line and animal model generation using benchmark strategies and optimized protocols.
      • Vector designing or cloning for RNAi/ inducible vectors homologous recombination, Bacterial Artificial Chromosome (BAC) Recombineering, de novo gene synthesis, site-specific mutagenesis, gene tagging, and more.
      Virus Packaging / DNA Cloning Categories

      Virus Packaging (Lentivirus and Retrovirus)

      Convenient Custom Lentivirus and Retrovirus Packaging Service that provides you with ready-to-transduce viral particles containing your construct.

      Virus Packaging (Lentivirus and Retrovirus)

      CRISPR / Cas9 Vector
      Design & Construction

      Full service CRISPR/Cas9 vector design, construction and gRNA validation.

      CRISPR / Cas9 Vector
      Design & Construction

      Vector Design / Cloning
      Service

      Comprehensive cloning services for CRISPR/Cas9, BAC and other genome editing technologies, for in vivo and in vitro applications.

      Vector Design / Cloning
      Service

      Bacterial Artificial Chromosome (BAC) Design
      and Cloning

      Custom services for designing, cloning and validating genetic-sequences of interest into a bacterial artificial chromosome (BAC) vector.

      Bacterial Artificial Chromosome (BAC) Design
      and Cloning

    • Immortalization Service


      Applied StemCell offers patient fibroblast immortalization service via the simian virus 40 T antigen.
    • Cell Line Irridation Service


      Applied StemCell offers gamma-irradiation service for irradiating cell lines (patient-derived, pooled, genome edited/ corrected cell lines) using a Nuclear Regulatory Commission (NRC)-approved Gamma-emission source to irradiate cells at a constant dose rate and to any desired extent of radiation. Irradiated cell line models are novel toolkits for applications such as generation of co-culture models using non-dividing/ feeder cells; to study mechanisms underlying cancer progression; wound healing and inflammatory responses after irradiation; screen drugs for enhancing irradiation sensitivity of tumor cells; and for xenograft tumor models.

      Our Cell Line Irradiation Service includes:

      1. Cell line expansion/ harvesting
      2. Cell line Irradiation (customized extent of irradiation)
      3. Cryopreservation
  • Tissue Samples & Primary Cells

    • Human Primary Cells


      Primary cells are isolated from tissues, including blood and bone marrow. The isolated human primary cells have the capacity to function similarly to cells in vivo under the right conditions, and they can be incorporated into various areas of research as model systems. Primary cells can be used for drug discovery, toxicity testing, gene therapy, cancer research, and more.

      In order to address the needs of the research community, Applied StemCell (ASC) now offers high-quality fresh and frozen human primary cells, including peripheral blood cells, cord blood cells, and bone marrow cells. ASC also provides hematopoietic stem cells, progenitor cells, and immune cells. For fresh cells, we offer on-demand, customized sample collection and processing services. All donors are IRB-approved and have been tested for Syphilis, Hepatitis B (HBV), Hepatitis C (HCV), HTLV-I/II, HIV-1, and HIV-2. Same-day delivery is available for Bay Area customers. Overnight delivery and international shipping are available for all other customers. Contact us today to learn more.

       

      Place Your Order Today!

      Ordering

      • Call 1-866-497-4180
      • Email: info@appliedstemcell.com
      • Contact Us
      • Order Online

      Ordering

      Collection

      • HIPPA & IRB Adherence
      • On-demand, customized sample collection
      • Request a specific donor
      • Customized aliquots 

      Collection

      Processing

      • Completed on-site in the U.S.
      • Our scientists have 20+ years of experience
      • Optimized protocols

      Processing

      Shipping & Delivery

      • Bay Area same-day delivery; Overnight delivery for all other U.S. customers
      • Worldwide shipping

      Shipping & Delivery

    • Human Hematologic Tissue Products


      Applied StemCell (ASC) now offers human hematologic tissue products, including human healthy whole/peripheral blood and cord blood samples. Plasma, Leukopak, and Off-the-clot serum products are available. ASC offers on-site procurement and processing. Our samples are collected from IRB-approved donors who have tested negative for Syphilis, Hepatitis B (HBV), Hepatitis C (HCV), HTLV-I/II, HIV-1, and HIV-2. Contact ASC today to learn more about customized sample collection and requesting a specific donor.

      Place Your Order Today!

      Ordering

      • Call 1-866-497-4180
      • Email: info@appliedstemcell.com
      • Contact Us
      • Order Online

      Ordering

      Collection

      • HIPPA & IRB Adherence
      • On-demand, customized sample collection
      • Request a specific donor
      • Customized aliquots 

      Collection

      Processing

      • Completed on-site in the U.S.
      • Our scientists have 20+ years of experience
      • Optimized protocols

      Processing

      Shipping & Delivery

      • Bay Area same-day delivery; Overnight delivery for all other U.S. customers
      • Worldwide shipping

      Shipping & Delivery

    • Non-Human Primate Cells & Hematologic Tissue Products


      Non-human primate (NHP) cells and hematologic products are now available. Our non-human primate samples are collected from healthy cynomolgus macaques or rhesus macaques that are housed in the United States. All of our products are from NHPs that have tested negative for SIV, STLV, SRV, and Herpes B. These high-quality NHP products can be used in disease modeling, toxicity testing, or other areas of research. You can schedule a free consultation today to discuss specific project requirements with one of our experts. You can request an NHP donor of a specific gender or age, and you can reserve the cynomolgus or rhesus donor for the entire duration of your project. Contact us today to learn more.

      Place Your Order Today!

      Ordering

      • Call 1-866-497-4180
      • Email: info@appliedstemcell.com
      • Contact Us
      • Order Online

      Ordering

      Collection

      • HIPPA & IRB Adherence
      • On-demand, customized sample collection
      • Request a specific donor
      • Customized aliquots 

      Collection

      Processing

      • Completed on-site in the U.S.
      • Our scientists have 20+ years of experience
      • Optimized protocols

      Processing

      Shipping & Delivery

      • Bay Area same-day delivery; Overnight delivery for all other U.S. customers
      • Worldwide shipping

      Shipping & Delivery

    • Human Diseased Products


      Applied StemCell (ASC) works closely with clinicians to obtain disease samples from patients who have been recently diagnosed or have not received treatment. ASC provides a diverse selection of human fresh and frozen diseased products that can be used for disease modeling, drug development, and other areas of research. Call us today to place your order. We will have the sample ready and, in your hands, the same day of collection when you order one week in advance. ASC offers same-day delivery to Bay Area customers, and overnight and international shipping is available to all customers. The availability of the diseased products is dependent on the prevalence of the disease. If you would like more information, contact us today!

      Place Your Order Today!

      Ordering

      • Call 1-866-497-4180
      • Email: info@appliedstemcell.com
      • Contact Us
      • Order Online

      Ordering

      Collection

      • HIPPA & IRB Adherence
      • On-demand, customized sample collection
      • Request a specific donor
      • Customized aliquots 

      Collection

      Processing

      • Completed on-site in the U.S.
      • Our scientists have 20+ years of experience
      • Optimized protocols

      Processing

      Shipping & Delivery

      • Bay Area same-day delivery; Overnight delivery for all other U.S. customers
      • Worldwide shipping

      Shipping & Delivery

    • Cytokines & Growth Factors


      Applied StemCell (ASC) now offers high purity cytokines and growth factors produced by HEK293T cells. We use novel technology to provide customers with high-purity human cytokines that have not been tagged during production. Additionally, our extensive purification methods allow us to generate human cytokines that exhibit super-low levels of endotoxins. ASC’s high purity and high activity cytokines allow you to avoid the higher levels of endotoxin contamination of cytokines from bacteria and other systems. Contact ASC today to speak with one of our experts to learn more about our active human-specific cytokines and which product best fits your project needs.

      Place Your Order Today!

      Ordering

      • Call 1-866-497-4180
      • Email: info@appliedstemcell.com
      • Contact Us
      • Order Online

      Ordering

      Collection

      • HIPPA & IRB Adherence
      • On-demand, customized sample collection
      • Request a specific donor
      • Customized aliquots 

      Collection

      Processing

      • Completed on-site in the U.S.
      • Our scientists have 20+ years of experience
      • Optimized protocols

      Processing

      Shipping & Delivery

      • Bay Area same-day delivery; Overnight delivery for all other U.S. customers
      • Worldwide shipping

      Shipping & Delivery

    • FFPE Cell & Tissue Products


      If you are looking to expedite your project timeline and you do not require customized FFPE slides, scrolls, or blocks, explore our new collection of FFPE cell pellets and tissues to discover the high-quality FFPE product that can help you drive your research forward. Applied StemCell provides FFPE human and animal cell pellets in various formats, including slides, blocks, and scrolls. Our technology enables the production of round, homogenous pellets that are evenly distributed throughout each section. Although we supply a diverse set of cell pellets with consistent densities, FFPE human and animal tissue products are also available for purchase. When you find what you are looking for, contact us and we will help you place your order.

      • Slides, blocks & scrolls
      • Cell Pellets & Tissue Samples 
      • Round, homogenous cell pellets slides and arrays
      • Affordable off-the-shelf products with the option to create your own customized FFPE slide, block, scroll, or array with our cost-effective service platform 

      Cell Lines

      Breast Lung Cervical Prostate
      SKBR3 
      MCF7
      MDA-MB-231
      MDA-MB-468 3
      BT474
      T47D
      MDA-MB-453
      Hs578T
      BT549
      ZR751
      BT20
      MDA-MB-175
      HCC1806
      HCC1143
      HCC70
      HCC1937
      HCC38
      DU4475
      H2228
      H23
      A549
      HOP62
      H322
      H1703
      H1975
      H838
      SK-MES-1
      HOP92
      H596
      H1650
      SW1573
      HCC78
      MRC5
      EKVX
      SW900
      H226
      H522
      H460
      DV90
      H520
      H2122
      H647
      H211
      H820
      H1755
      H441
      H358
      H1299
      H1993
      H1648
      HCC827
      H841
      HCC827GR6
      HELA
      CASKI
      SIHA
      C33A
      HT3
      MS751
      ME180
      PC3
      DU145
      22RV1
      LNCAP

       

      Colorectal Skin
      SW620
      HT29
      LS174T
      COLO205
      HCT116
      HCT15
      SNU-C2B
      SNU-C1
      SW48
      SW1116
      SW403
      CACO2
      Colo201
      SW1417
      SW948
      LOVO
      SW480
      SK-MEL-2
      M14
      CHL1
      SK-MEL-28
      C32
      HT144
      MALME-3M
      UACC62
      SK-MEL-5
      A431
      HMCB
      SK-MEL-30
      OPM-2
      A375
      Detroit551
      BJ-5TA

       

      Leukemia Lymphoma Ovarian Kidney
      K562
      JURKAT
      HL60
      CCRF-CEM
      MOLT4
      RPMI-8226
      THP1
      THP1
      MEC1
      SUDHL1
      SUDHL4
      HUT78
      SUDHL6
      RI-1
      WSU-NHL
      RAMOS
      MM1S
      DAUDI
      ST486
      U937
      HDLM-2
      U266
      FARAGE
      SR
      RAJI
      MV411
      Pfeiffer
      OVCAR8
      IGROV1
      PA1
      CAOV3
      SKOV3
      SW626
      OVCAR3
      OVCAR3
      786-O
      HEK293
      293T
      A498
      CAKI-1
      ACHN
      A704
      769P

       

      Liver Pancreatic Brain Stomach Head & Neck Uterus Bladder Vulva
      HEPG2
      HUH7
      HEP3B
      SNU475
      BXPC3
      MIAPACA2
      PANC1
      PSN1
      PL45
      ASPC1
      CAPAN2
      SW1990
      U118MG
      A172
      PFSK1
      SW1088
      DAOY
      U87MG
      U251MG
      SF295
      SF295
      DBTRG-05MG
      SNU16
      SNU16
      KATOIII
      Hs746T
      N87
      SNU5
      DETROIT-562
      FADU
      SCC90
      SCC152
      SCC25
      AN3CA RT4
      SW780
      HT1197
      SKLMS1

       

      Bone Thyroid Retroperitoneum Muscle Esophagus Placenta
      U2OS
      SH-SY5Y
      MG63
      KG1 (CCL-246)
      SJSA1
      Thyroid SKPNDW
      93T449
      A673 OE19
      KYSE70
      JEG3

       

      Animal
      3T3
      CHO
      CHO-K1
      CHO-K1
      NRK-49F
      NRK-49F
      aTC1 clone 6
      EML
      beta-TC-6
      MC/9
      NE-4C
      B16-F10
      C8-D1A
      CW13.20-3B3
      CW13.20-3B3
      EMT6
      AML12
      LL/2 (LLC1)
      N1E-115
      CATH.a
      BW5147.3
      MC38
      Raw 264.7
      bEND.3
      Neuro2a
      CT26

       

      CPA Cell Lines Included
      Breast MCF7, BT474, SKBR3, MDA-MB-468, MDA-MB-231
      Lung A549, Hcc78, H1975, SKMES1, H1703, H596, HOP62, HOP92
      Prostate PC3, DU145, 22RV1, LNCAP
      Colorectal SW620, LS174T, COLO205, HT29, HCT116, HCT15
      Leukemia/Lymphoma JURKAT, CCRF-CEM, K562, RAJI, HUT78, SUDHL1, SUDHL4
      Pancreatic PANC1, MIAPACA2, BXPC3, PSN1
      Skin CHL1, C32, M14, SKMEL2, SKMEL28
      HER2 HER2-0+, HER2-1+, HER2-2+, HER2-3+
      PD-L1 PD-L1-, PD-L1+

      Dont's see what you are looking for? Contact us today!

       

       

       

       

    • Custom Primary Cell Isolation Services


      Applied StemCell can isolate primary cells from fresh tissues, including from biopsy samples.

    • Custom FFPE Cell Pellet & Tissue Blocks, Slides, Scrolls and Arrays


      Whether you are looking for formalin fixed paraffin embedded (FFPE) blocks, slides, scrolls, or arrays, we can generate the preserved human or animal product you need for your basic research, disease studies, or drug development project with our collection of customizable cell pellet and tissue FFPE services. Unlike other providers, our unique protocols enable the creation of round cell pellets with a consistent density in every section. If you are looking to preserve a specific animal tissue, our scientists can not only tailor your array to your core size and orientation requirements, our team can combine your cell pellet and tissue samples into a single array. Furthermore, you have the power to further personalize your service by selecting a different cell pellet shape (square, rectangle, etc.) and density (1-90%) or tissue thickness and length. If you would like to learn more, visit our service pages or contact us to schedule your free consultation.

      ASC Service Advantages

      • Customizable: size (2, 3, 7, 10 mm), shape (circle, rectangle, etc.) density (1-90%), mixing & array (core size, orientation, etc.) options
      • Several formats to choose from: single pellet slides, pellet array slides, on-slide control arrays, scroll
      • Alternative tissue “stick” options for researchers with limited samples
      • Fast & Affordable

       

      Service Options

      FFPE Cell Pellet (Blocks/Slides/Scrolls)

      - Single Pellet Slides
      - Pellet Array Slide
      - On-Slide Control Array
      - FFPE Scroll
      - Custom Services

      FFPE Cell Pellet (Blocks/Slides/Scrolls)

      FFPE Tissue (Single/Array)

      - Mouse & Rat Tissues Available
      - Custom Array Capabilities
      - "Stick" Service Available

      FFPE Tissue (Single/Array)

      Start Your Project

      - Email: info@appliedstemcell.com
      - Call: 1-866-497-4180
      - Contact Us

      Start Your Project

  • Antibody Discovery & Bioproduction

    • TARGATT™ HEK293 and CHO Cell Protein Expression


      TARGATT™ technology enables the site-specific integration of any gene-of-interest (GOI; up to 20kb) at a preselected safe harbor locus with the use of a unique integrase. Applied StemCell has generated many TARGATT™ cell lines, including the TARGATT™ CHO and HEK293 Master Cell Lines. These high-quality cells are now part of ready-to-use TARGATT™ knock-in kits that you can use to insert your GOI in your own lab. We have observed steady, medium to high levels of protein expression when using our master cell lines for customer projects, so you can confidently purchase any kit that best suits your research needs. Additionally, our experts have used engineered TARGATT™ cells to address common bioproduction issues.

      The traditional CHO antibody and animal bioproduction methods are inefficient because they require random gene insertion and forced amplification of transgenes. Applied StemCell has addressed these problems by utilizing our expertise in cell line and animal model engineering with our proprietary TARGATT™ technology to develop two bioproduction methods: TARGATT™ CHO Master Cell Line and Transgenic TARGATT™ Rabbits. Both methods offer low cost, efficiency, consistent protein expression and high protein yield that is easily scalable for large-scale bioproduction.

      Advantages of the TARGATT™ Master Cell Line:

      • High knock-in efficiency
      • Site-specific integration into the H11 genomic hotspot well-defined safe harbor locus
      • Single gene knockin: one variant - one locus - one cell line
      • Unidirectional integration for stable knock-in cell lines
      • Uniform, high-level gene expression
      • Overcomes challenges such as random insertion, gene silencing, multiple copy gene integration, ablated gene expression.
      • TARGATT™ CHO and HEK293 Master Cell Lines
      • Transgenic TARGATT™ Rabbits for Bioproduction

      If you would like to work with your own cell line, our experts can engineer the TARGATT system into your cell line. To learn more, explore our Custom TARGATT Master Cell Line Generation page or contact us today to schedule your free consultation.

    • Antibody Discovery: TARGATT™ HEK293 and CHO Cell-Based Libraries and Monoclonal Antibody Development


      Explore ASC’s series of antibody services and discover how we can help you propel your research forward. Whether you are looking to generate monoclonal antibodies or construct a cell-based library for screening, we have several well-established protocols and fully customizable service options for you to select from. Our single B cell antibody discovery platform is great for those seeking high-affinity rabbit monoclonal antibodies while our TARGATT™ HEK293 and CHO cell lines are ideal tools for researchers aiming to establish stable knock-in cell lines or large cell libraries. Visit our service pages or contact us today to learn more.

      Advantages of the TARGATT™ Master Cell Lines

      • High knock-in efficiency
      • Site-specific integration into the H11 genomic hotspot well-defined safe harbor locus
      • Single gene knockin: one variant - one locus - one cell line
      • Unidirectional integration for stable knock-in cell lines
      • HEK293 and CHO cells for library construction

      Advantages of ASC's Single B Cell Antibody Discovery Platform

      • Unique animal strains/lines (rabbit & alpaca)
      • Accelerated and Classical immunization
      • Amplification of Single B Cell mRNA Efficient Mammalian Cell Antibody Expression

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