Research

  • Services

    • Stem Cell Services


      Accelerate your research and discoveries by leveraging our extensive expertise in stem cell technology! We can provide physiologically relevant in vitro models derived from Pluripotent Stem Cells (PSC) for more predictive models of human disease and biology. ASC offers a comprehensive custom service platform for every aspect of stem cell-based research: iPSC generation/ ESC derivation, CRISPR/Cas9 genome editing and cell line model generation; differentiation to somatic lineage of choice, downstream assays to validate your cell line and for drug discovery and screening.

      Stem Cell Services Categories

      iPSC Generation
      from Patient Samples

      Custom iPSC generation from healthy/ disease PBMCs or fibroblasts using episomal, mRNA, other integration-free or retroviral reprogramming methods.

      iPSC Generation
      from Patient Samples

      Stem Cell
      Genome Editing

      Custom mutation engineering & correction in control orpatient iPSCs using CRISPR/Cas9 for predictive in vitro modeling of human biology/disease.

      Stem Cell
      Genome Editing

      Safe Harbor Locus Master
      iPSC Genome Editing Service

      Custom isogenic and reporter iPSC lines generated using our site-specific TARGATT™ gene integration technology and TARGATT™ iPSC Master cell line.

      Safe Harbor Locus Master
      iPSC Genome Editing Service

      Neural Stem Cell
      Differentiation

      Service for ESC / iPSC differentiation into Neural Stem Cells (NSCs) , Motor Neurons and Glial Cells (Astrocytes and Oligodendrocytes).

      Neural Stem Cell
      Differentiation

      Cardiomyocytes
      Differentiation

      Comprehensive service to differentiate iPSCs into high purity, functional cardiomyocytes from healthy/ disease samples.

      Cardiomyocytes
      Differentiation

      Hepatocytes
      Differentiation

      Custom differentiation of iPSCs to mature and robust population of ready-to-use hepatocytes.

      Hepatocytes
      Differentiation

      Teratoma Analysis ,
      iPSC Characterization

      Services to identify & characterize Pluripotent Stem Cells (iPSC/ ESC): Teratoma Analysis, Pluripotency Marker Assays, qPCR, RNA-seq & more.

      Teratoma Analysis,
      iPSC Characterization

      iPSC Culture
      Services

      We offer standard and customized services for culture expansion, scale-up, and 3D culture morphology testing of iPSC and differentiated cells.

      iPSC Culture
      Services

      Stem Cell
      Derivation

      Mouse embryonic stem cell (ESC) derivation service from any mouse strain, including derivation of isogenic cell lines from transgenic mice.

      Stem Cell
      Derivation

    • Neurotoxicity Screening


      Cell-based in vitro assays for drug toxicity are becoming crucial tools to screen new drug candidates because they are inexpensive, efficient, and ethically compatible preliminary screening alternatives to the more expensive animal testing models.

      Applied StemCell offers a one-stop shopping solution for neurological compound screening. Starting with the engineering of iPSCs from healthy and diseased patient samples; developing disease models via gene editing for drug target discovery and efficacy testing; differentiation into cell lineage(s); cell line model characterization; and ending with a comprehensive panel of cell-based tests for drug efficacy, neurotoxicity, and target discovery that are regulatory compliant.

    • CRISPR/Cas9 Genome Editing


      Save Your Time, Money & Effort! Leverage our extensive expertise in CRISPR/Cas9 genome editing technologies to generate genetically modified mouse, rat, cancer and stem cell lines, with a variety of modifications in a targeted gene of interest. As one of the earliest licensees of CRISPR/Cas9 technology, we have genetically engineered > 1800 unique cell line and animal models for disease modeling, functional genomics, target identification, antibody validation, and validation for drug discovery and screening, and more. We offer affordable, comprehensive custom service with a fast turnaround time to meet the exact requirement of your projects. You can also combine it with our downstream custom assay services for a seamless project workflow, all from our US facility.

      CRISPR/Cas9 Genome Editing Categories

      Cell Line Models

      Custom stable cell line model generation using CRISPR/Cas9 for disease modeling, antibody validation, and drug screening.

      Cell Line Models

      Mouse Models

      Custom CRISPR/Cas9 engineered mouse models with a variety of genetic modifications; generated in our AAALAC-accredited animal facility in the USA.

      Mouse Models

      Rat Models

      Custom CRISPR/Cas9 rat models for disease modeling and drug screening; generated in our AAALAC-accredited animal facility in the USA.

      Rat Models

    • TARGATT™ Genome Editing


      Applied StemCell’s proprietary TARGATT™ technology, enables site-specific, stable integration of large DNA fragments into a safe harbour locus more efficiently and faster, with guaranteed transgene expression. The TARGATT™ gene-editing platform is versatile and can generate large fragment knock-in animal or cell line models. This technology circumvents problems associated with random integration such as position effect, and gene silencing or instability due to integration of multiple copies of the transgene. Applications for TARGATT™ models: Transgene overexpression models, reporter gene knock-in, conditional knock-in, inducible expression, Cre-driver lines and humanized animal models.

      TARGATT™ Genome Editing Categories

      TARGATT™ Site Specific
      Knock-in Mouse

      Fast and Reliable! Site-specific knock-in mouse model generation service for large transgene insertion.

      TARGATT™ Site Specific
      Knock-in Mouse

      TARGATT™ Site-Specific
      Knock-in Rat

      TARGATT™ Site-Specific Knock-in Rat (H11) model generation service for large fragment gene insertion into Sprague-Dawley rats.

      TARGATT™ Site-Specific
      Knock-in Rat

      TARGATT™ Site-Specific
      Knock-in Cell Line Service

      Efficient generation of stable, site-specific, gene knock-in in mammalian cell lines and stem cells.

      TARGATT™ Site-Specific
      Knock-in Cell Line Service

      TARGATT™ High Resolution Protein Screening

      Site-specific, isogenic cell libraries for consistent expression of gene/protein variants for genome-wide screening and protein evolution.

      TARGATT™ High Resolution Protein Screening

    • Bioproduction


      The traditional CHO antibody production and animal bioproduction methods are inefficient because they require random gene insertion and forced amplification of transgenes. Applied StemCell has addressed these problems by utilizing our expertise in cell line and animal model engineering with our proprietary TARGATT™ technology to develop two bioproduction methods: TARGATT™ CHO Master Cell Lines and Transgenic TARGATT™ Rabbits. Both methods offer low-cost, efficiency, consistent protein expression and high protein yield that is easily scalable for large scale bioproduction.

      Bioproduction Categories

      CHO Cells

      Site-specific transgene knock-in using TARGATT CHO Master Cell Lines for the creation of high protein/antibody expression in bioproduction cells.

      CHO Cells

      Transgenic Animals - Rabbit

      Applied StemCell's animal bioproduction platform uses transgenic TARGATT™ Rabbits to consistently express recombinant proteins at a very high yield.

      Transgenic Animals - Rabbit

    • Lentivirus Stable Cell Line Generation


      A comprehensive custom Lentiviral-based Stable Cell Line Generation Service for genetically engineering sensitive cells, primary cells, stem cells and terminally differentiated cells. Lentivirus-based stable cell line generation offers a cost-effective and efficient alternative for generating stable cell line models for gene overexpression, inducible gene expression, gene knockout and gene knockdown.

      Key Features:

      • Modified lentiviruses with broad tropism to target most mammalian species and cell types.
      • Non-integrating lentiviruses for CRISPR-lenti gene knockout.
      • Choice of selection markers: antibiotic resistance or GFP for FACS.
      • Use 3rd generation lentiviral systems for the highest biosafety standards.
      • Gene editing confirmed by NGS or RT-PCR.
    • DNA Cloning & Virus Packaging


      With decades of experience our Molecular Biology Team offers an exceptional customized DNA cloning service for vector design and cloning strategies. We can help you develop the best strategy to tackle your cloning projects and find solutions to all your technical problems.

      • Cloning of amplified DNA fragments
      • RNAi and inducible vectors
      • Gene targeting vectors for homologous recombination
      • Site-specific mutagenesis
      • Bacterial Artificial Chromosome (BAC) Recombineering
      • De novo gene synthesis
      • Tagging of genes

      If you don’t see what you want, call us!

      DNA Cloning / Virus Packaging Categories

      Virus Packaging (Lentivirus and Retrovirus)

      Convenient Custom Lentivirus and Retrovirus Packaging Service that provides you with ready-to-transduce viral particles containing your construct.

      Virus Packaging (Lentivirus and Retrovirus)

      CRISPR / Cas9 Vector
      Design & Construction

      Full service CRISPR/Cas9 vector design, construction and gRNA validation.

      CRISPR / Cas9 Vector
      Design & Construction

      Vector Design / Cloning
      Service

      Comprehensive cloning services for CRISPR/Cas9, BAC and other genome editing technologies, for in vivo and in vitro applications.

      Vector Design / Cloning
      Service

      Bacterial Artificial Chromosome (BAC) Design
      and Cloning

      Custom services for designing, cloning and validating genetic-sequences of interest into a bacterial artificial chromosome (BAC) vector.

      Bacterial Artificial Chromosome (BAC) Design
      and Cloning

    • CRO Services


      As a leader in genome editing and stem cell technologies, Applied StemCell provides ISO:9001 quality, customizable solution-oriented services for preclinical development. We offer customized drug discovery screening, downstream assays in animal models, cell line irradiation, and other custom research services so you can focus on the main aspects of your research. We will achieve your unique requirements by customizing each part of your project to fit the stage of your research.

      CRO Services Categories

      Cell Line Model Generation

      Custom stable cell line model generation using CRISPR/Cas9 for disease , antibody validation, and drug screening.

      Cell Line Model Generation

      Cell-Based Assays

      Customizable, comprehensive panel of cell-based assays to assays that validate, evaluate cell line function and behavior and more.

      Cell-Based Assays

      Drug Toxicity and Efficacy Testing

      We offer custom in vitro disease models for safety and efficacy testing of early, preclinical screening of potential drug candidates.

      Drug Toxicity and Efficacy Testing

      Custom In Vivo Assay
      Services

      Applied StemCell offers fully customizable platform for downstream assays in animal models.

      Custom In Vivo Assay
      Services

      Cell Line Irradiation
      Service

      Applied StemCell offers custom gamma (g)-irradiation service for patient-derived, pooled, genome edited/corrected human and animal cell lines.

      Cell Line Irradiation
      Service

      Custom Services

      We provide the following preclinical development services and support: Fibroblasts Immortalization, Custom Primary Cell Isolation, and FFPE Service.

      Custom Services

  • Products

    • Stem Cell


      Applied StemCell has a comprehensive catalog of products for every aspect of your stem cell research:

      • Ready-to-use induced pluripotent stem cell (iPSC).
      • iPSC-derived differentiated neural lineage cells.
      • For the more hands-on researcher, we offer protocols and kits for iPSC generation to generate your own iPSCs.
      • To help you maintain your stem cells, we also offer high quality and thoroughly tested mouse embryonic fibroblasts (MEF) feeder cells, and stem cell-grade “pre-validated“ FBS.
      Stem Cell Categories

      iPSC & ESC Lines

      iPSC & ESC Lines

      Ready-to-use, fully characterized human iPSCs from different sources and clinical conditions, iPSC from other mammalian species, and mouse ESC lines.

      iPSC & ESC Lines

      iPSC Differentiated Cells

      We offer fully-characterized iPSC-differentiated progenitors and cell lines: Neural stem cells (NSC), neurons, astrocytes, and cardiomyocytes.

      iPSC Differentiated Cells

      Genome Edited iPSC Lines

      Control iPSC-derived isogenic panels of neuronal gene knockout, lineage-specific reporter knock-in, and safe harbor locus reporter knock-in iPSC lines.

      Genome Edited iPSC Lines

      Stem Cell Culture:
      MEF Feeder Cells, FBS

      Validated, high quality MEF feeders and ESC-Sure™ FBS for robust growth of your stem cells.

      Stem Cell Culture:
      MEF Feeder Cells, ESC-Grade FBS

      iPSC Generation
      Kit

      Reprogram your own iPSCs from your somatic cells using either our Human EZ- iPSC Retroviral or Episomal Generation kits.

      iPSC Generation
      Kit

    • TARGATT™ Genome Editing


      TARGATT™ site-specific knock-in technology enables the integration of transgenes at a preselected locus in the genome, thus overcoming drawbacks of random integration. This technology is adaptable for gene integration in many different species of animals (mice, rats, rabbits, pigs) and cell lines (including CHO cells, stem cells and immortalized cell lines).

      Applications:

      • Inducible expression models
      • Reporter gene insertion models
      • Transgene overexpression models
      • Humanized animal models
      • Cre - driver lines
      TARGATT™ Genome Editing Categories

      TARGATT™ Mouse Model Generation

      Do-it-yourself kits to generate site-specific transgenic mice in 3 months! We offer TARGATT™ “attP” mice, plasmids, transgenic kits, genotyping kits.

      TARGATT™ Mouse Model Generation

      TARGATT™ Knockin
      Master Cell Line

      TARGATT™ “attP” Master cell lines and knock-in kit for rapid, site-specific transgene knock-in with high efficiency and robust transgene expression.

      TARGATT™ Knockin
      Master Cell Line

    • Genetically Modified Cell Lines / Isogenic Cell Lines


      Isogenic Cell Lines for Drug Discovery: Isogenic cell lines represent a unique toolset for studying the impact of genotype on cellular phenotype. An isogenic cell line is a cell line that has been engineered from a parental line through the introduction of a targeted gene mutation. In doing so, the parental cell line inherently becomes a control line to which the engineered line can be referenced. Isogenic cell line pairs can be incredibly useful as tools for studying cellular biology, such as the impact of genotype on cellular phenotype, as well as for parallel, high throughput screening to enable the discovery of therapeutic compounds that exhibit genotype-specific toxicity.

      Applied StemCell offers a number of homozygous isogenic cell lines.

      Genetically Modified Cell Lines / Isogenic Cell Lines Categories

      Neuronal Disease Specific Isogenic Knockout iPSCs

      Fully characterized, isogenic neuronal gene knockout iPSCs engineered from control iPSC line (ASE-9109), ideal for neurological disease modeling.

      Neuronal Disease Specific Isogenic Knockout iPSCs

      Reporter iPSC Lines
      (Isogenic)

      Ready-to-use, isogenic panels of knock-in reporter iPSC lines (neuronal lineage & safe harbor locus) generated from control/ master iPSC (ASE-9109).

      Reporter iPSC Lines
      (Isogenic)

    • Cancer Research Tools


      Applied StemCell has developed stress-free, cost saving solutions for cancer research. Our revolutionary approach to optical imaging has replaced the traditional method of bioluminescence with a novel luciferase system that allows cells to continuously and autonomously produce a bioluminescent signal. Harness the power of autonomous bioluminescent cells for efficient research with xenograft animal models.

      Applied StemCell’s Cancer Research Tools are ideal for:

      • Preclinical drug screening and drug discovery.
      • Toxicity testing.
      • Metabolic activity monitoring.
      • Tumorigenesis and treatment studies.
      • Estrogenic activity screening.
      • Non-invasive in vivo tumor tracking in small animal models
      Cancer Research Tools Categories

      Substrate-free
      Autobioluminescent Cell
      Lines and Vectors

      Cost-saving and time efficient bioimaging with an substrate-free bioluminescent reporter engineered in 4 major cell lines: HCT116, HEK293, MCF7, T47D.

      Substrate-free
      Autobioluminescent Cell
      Lines and Vectors

    • Sample Prep


      Applied StemCell provides Human Fibroblast Immortalization Kit for a do-it-yourself alternative to generate your own immortalized cell lines. The Human Fibroblast Immortalization Kit is a mixture of polymers optimized for the infection of lentivirus or retrovirus in most cells, and can increase transduction rate up to 10-fold. Culture with confidence! 

      Sample Prep Categories

      Human Fibroblast
      ImmortalizationKit

      ASC’s Human Fibroblast Immortalization kit contains a Virus Transduction Enhancer that increases transduction by 10-fold for lentiviruses in most cells.

      Human Fibroblast
      Immortalization Kit

  • Animal Models

    • Mouse Models


      ASC is a leading provider of genetically engineered mouse models for biomedical research and drug discovery. Our custom mouse model generation service includes:

      • F1 breeding for germline transmission
      • Mouse models engineered in an AAALAC-accredited animal facility in the USA
      • Two complementary gene editing technologies, CRISPR and TARGATT™ for precise and advanced physiologically relevant mouse models
      • New! surgically/ chemically induced mouse models of neurological diseases
      • Customized downstream projects for in vivo assessments (automated behavior/ locomotor activity, EEG/ ECG, and pharmacokinetics) as well as in vitro evaluations (electrophysiology, immunohistochemistry, RT-PCR, western blots and more)
      • Dedicated project management and timely reports
      Mouse Models Categories

      Conditional Knockout
      Mouse Models

      Conditional knockout/ expression mouse model generation for a variety of temporal and spatially controlled gene expression in mice.

      Conditional Knockout
      Mouse Models

      Site-Specific TARGATT™
      Knock-in Mouse Models

      Fast and Reliable! Site-specific, large transgene knock-in in a safe harbor locus for gene overexpression and custom Cre mouse line generation.

      Site-Specific TARGATT™
      Knock-in Mouse Models

      Knockout, Knock-in, Point
      Mutation Mouse Models

      Custom mouse model generation with biorelevant gene knockout, knock-in, and point mutation modifications for basic and preclinical research.

      Knockout, Knock-in, Point
      Mutation Mouse Models

      Site-Specific Knock-In TARGATT “attP” Mice

      TARGATT™ attP mice available at Applied StemCell, along with associated kits enable you to make site-specific transgenic mouse models in your lab.

      Site-Specific Knock-In TARGATT “attP” Mice

    • Rat Models


      Genetically engineered rat models are gaining popularity as the preferred biological model for several research areas. ASC can genetically engineer rat models with a physiologically relevant modifications using an expanded technology portfolio with complementary CRISPR/Cas9 and TARGATT™ technologies, as well as, traditional homologous recombination and random transgenic technologies.

      • ISO:9001 quality service
      • F1 breeding for germline transmission
      • AAALAC-accredited animal facility in the USA
      • New! surgically/ chemically induced rat models of neurological diseases
      • Customized projects for in vivo assessments (automated behavior/ locomotor activity, EEG/ ECG, and pharmacokinetics) as well as in vitro evaluations (electrophysiology, immunohistochemistry & other biochemical assays)
      Rat Models Categories

      Conditional Knockout
      Rat Models

      Conditional knock-out/ expression mouse model generation to accurately model temporal and spatially controlled gene expression in rats.

      Conditional Knockout
      Rat Models

      Site-Specific TARGATT™
      Knock-in Rat Models

      Fast and Reliable! Site-specific, safe harbor locus large transgene knock-in for gene overexpression and custom Cre rat line generation.

      Site-Specific TARGATT™
      Knock-in Rat Models

      Knockout, Knock-in, Point
      Mutation Rat Models

      Full service CRISPR rat model generation with precise gene knockout, knock-in, and point mutation modifications for basic and preclinical research.

      Knockout, Knock-in, Point
      Mutation Rat Models

    • Custom In Vivo Assay Services


      As a long-standing leader in genetically engineered animal models, Applied StemCell also offers a comprehensive custom research solutions platform for downstream assays in mouse and rat models. Our state-of-the-art vivarium is equipped with automated cages & devices for behavioral assessments, sample collection and in vivo measurements (ECG, EEG) of the animals. We have a multidisciplinary team of expert scientists who can design a comprehensive project plan to fit any requirement/stage of your research pipeline and for drug discovery and screening.

      • Designing and engineering research animal disease models
      • Adoptive transfers/transplantation
      • In vivo functional screening assays
      • End-of-study in vitro assays
  • Cell Line Models

    • Stem Cells - iPSC and ESCs


      Applied StemCell’s stem cell division has scientists with >75 years of combined experience in stem cell technology. We provide the most comprehensive stem cell related service and product platform for every stage of your stem cell research. Our stem cell service platform makes use the latest and most optimized protocols for efficient and successful projects. Custom stem cell service encompasses stem cell generation, disease modeling, differentiation, characterization to downstream validation of your models and drug screening assays. Our stem cell product catalog includes a variety of ready-to-use, well-characterized iPSC lines, pre-differentiated isogenic panels of neural lineage cells, MEF feeder cells and stem-cell grade FBS among other ISO:9001 quality products.

    • Cancer Research


      We know the ins-and-outs of genome editing in cancer cell lines having generated 1300+ cell line models in >200 distinct cell lines. ASC provides expert, high quality enabling tools for cancer research such as precision genome editing to generate physiologically relevant immuno-oncology cell line models and pre-engineered/ isogenic cell lines for early stage drug toxicity screening, generating your own isogenic cell lines and more.

      • Multi-approach strategies using complementary CRISPR/Cas9, TARGATT™, and lentiviral technologies
      • Engineer a variety of genetic modifications in a wide range of mammalian cell lines
      • Customized deliverables and downstream assay/screening services.
      • Short turnaround time (as fast as 2 months)
    • Antibody Validation


      The Ultimate Negative Controls for Genetic Validation of Your Antibodies! Evaluate antibody/ epitope specificity and non-specific binding in gene knockout cell lines. As a leading provider of custom CRISPR Cell Line Genome Editing services, ASC uses highly optimized CRISPR designing strategies and multi-approach protocols to generate frameshift mutations or precisely deleted targeted region(s) of the gene in any cell line of interest, to serve as the ideal negative controls for your antibody validation assays:

      • Preliminary expression level confirmation by RT-PCR or Western Blotting
      • Gene targeting and knock-out cell line engineering using CRISPR/Cas9
      • Optional KO validation by locus-specific sequencing, RT-PCR, or western
      • Downstream integration of KO cell lines into FFPE Blocks
    • UNISELECT™ Antibody Discovery and Screening


      UNISELECT™ Mammalian Cell-Based Membrane Proteins Antibody Discovery & Screening! Applied StemCell provides Custom TARGATT™ Master Cell Generation in your cell lines, and ready-to-use TARGATT™ HEK293, and TARGATT™CHO  to build protein libraries using site-specific transgene integration at a preselected safe harbor locus.

      This is a fast, unique and efficient platform for biopanning, including bispecific mAbs, antibody discovery/ screening, membrane proteins, CAR-T cell screening, and for bioprocessing.

      • Single copy integration
      • Homogenous expression of protein variants
      • Preselected safe harbor locus
      • Stable protein expression
      • No bacterial backbone insertion
      • Inducible expression compatible for membrane proteins (optional)
    • Cell-Based Assays


      Cell based assays have become an integral part of preclinical studies to elucidate biological mechanisms, and for screening drugs for efficacy, toxicity or combination analysis for drug synergy or antagonism. The use of live cells in these assays provide a fast and biologically relevant source of information. Applied StemCell offers a fully comprehensive panel of Cell-Based Assays from cell line expansion, cell line model generation, validation, to assays that evaluate cell line function and behavior. We have extensive experience in culturing and handling a wide variety of cell lines including cancer cell lines, immortalized cell lines, stem cells and differentiated cell lines. We will work with you at every stage of your project to fit it to the exact needs of your research.

    • Cell-Based Drug Toxicity and Efficacy Testing


      Make Informed Go-No-Go Decisions Early in Your Drug Development! Process! Applied StemCell (ASC), an ISO-certified service provider offers a flexible assay platform with a wide-range of functional endpoints for early-stage in vitro screening of preclinical drug candidates. We use a comprehensive cell-based test battery from which you can choose assays for efficacy, safety or target discovery that suit your therapeutic pipeline. We will provide the scientific expertise, accurate and efficient screening to help you take informed decisions about your small/ large molecules early in your drug development process to avoid costly drug attrition. Our custom screening service is ideal for CNS, cardiovascular, metabolic, cancer, and immunotherapy drug candidates.

    • Bioproduction Services- High Yield CHO Cells


      TARGATT™ for Bioproduction of Recombinant Proteins in Chinese Hamster Ovary (CHO) cells. The traditional CHO-cell based protein production is inefficient because they require random insertion and forced amplification of transgenes. ASC’s Master TARGATT™ CHO cell lines enable site-specific, single-copy insertion of large transgenes into a preselected safe harbor locus in the CHO cell genome, for guaranteed gene expression without disruption of internal genes. This site-specific TARGATT™ technology overcomes challenges posed by traditional random insertion methods, and results in high integration efficiency, stable knock-in cell lines, high levels of transgene expression, and reproducibility of clones. The Master TARGATT™ CHO cell lines are ideal antibody and recombinant protein expression.

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!