Rat Models

 

button-ko-ki-pm-rat-models     button-conditional-ko-rat-model     button-TARGATT-rat-models

Rats and mice have been used as model organisms for biomedical research for more than 150 years. In the last 30 years, however, mouse models became the more widely used animal model due to the ease of genetic manipulation of the mouse genome to generate genetically engineered mice as compared to rats. The successful isolation of rat embryonic stem cells (rES) and the subsequent availability of site-specific gene editing methods such as ZFNs, CRISPR, homologous recombination and TARGATT™ integrase technologies, have now enabled the efficient genetic manipulation of rat models. Indeed, engineered rat models are gaining popularity as the preferred model for certain research areas - cardiovascular, neuroscience, behavioral pharmacology, and more- for which genetically or physiologically relevant mouse models have been difficult to engineer.

Applied StemCell is a global leader in not only site-specific gene editing technologies such as CRISPR/Cas9 and TARGATT™, but also in animal model generation. We can generate a unique and ideal rat model for your research specifications efficiently and cost-effectively so you can enjoy stress-free research and discoveries.

Why do we need transgenic rat models?

  • Mouse models are not always reliable in preclinical studies and have several limitations for modeling human diseases
  • Rats were once the most widely used animal models in biomedical research until they were displaced by the transgenic mouse about 30 years ago.
  • The metabolism, physiology and pathology of rats are closer to human than mice
  • Larger size of rats allows for sophisticated surgeries, instrumentation and manipulations
  • Better behavioral models for cognition and memory, neurological and psychological assays and drug screening
  • Superior model for cardiovascular diseases (stroke and hypertension), autoimmune disorders, diabetes,
  • breast cancer, and autoimmune disorders
  • Knockout, Knock-in, Point Mutation Rat Models

    Applied StemCell leverages its several years of expertise in mouse and rat model engineering, and CRISPR/Cas9 technology to provide full service rat model generation for gene knockout, knock-in or point mutations to accurately model human diseases and physiology for advancing biomedical research and preclinical studies.

  • Conditional Knockout Rat Models

    ASC provides custom service to generate conditional knockout rat models to accurately model temporal or tissue-specific gene expression and disease pathology, for basic research as well as preclinical drug discovery and screening applications.

  • Large Transgene Knock-in Rat Models

    Fast and Reliable! Site-specific knock-in rat model generation service for safe harbor locus large transgene insertion, gene overexpression and custom Cre rat line generation.

 

Comprehensive Technology Platform for Gene Editing

Methods

Technical Advantage

TARGATT™ phiC31 integrase

  • Site-specific integration (H11 or ROSA26)
  • Works for large DNA Knock-in (~22kb)
  • High efficiency (up to 40%)
  • Insert promoter of choice for gene overexpression/ inducible expression

CRISPR / Cas9

  • High specificity
  • High efficiency in knockout, point mutation, and conditional knockout
  • Ease of use
  • Works for large DNA knock-in (-10kb)

We also offer rat model generation service using an expanded technology portfolio such as traditional homologous recombination, bacterial artificial chromosome and random transgenic technologies. With our expertise in rat model generation service and various genome editing technologies, we can assure you a custom genetically engineered rat model perfect for your research needs.

Random Integration Knock-in Rat Models