SNL 76/7 (STO Line)
Isolated from mouse fibroblast STO cell line transformed with neomycin resistance and murine LIF genes.
Mouse embryonic stem cells (ESCs) and induce pluripotent stem cells (iPSCs) generally require culture on feeder cells as well as medium containing Leukemia Inhibitory Factor (LIF) to maintain pluripotency and the capacity to self-renew. The SNL 76/7 cell line, derived from a mouse fibroblast STO cell line transformed with neomycin resistance and murine LIF genes, can be used as feeder cells for mouse and human ESC and iPSC culture. The SNL cells are sensitive to HAT selection (hypoxanthine, aminoprotein and thymidine) and negative for HPRT (hypoxanthine guanine phosphoribosyl transferase). Before use, SNL 76/7 cells must be mitotically inactivated by γ-irradiation or mitomycin-C treatment.
SNL 76/7 (STO Cell Line)
- Yang, J., Ryan, D. J., Lan, G., Zou, X., & Liu, P. (2019). In vitro establishment of expanded-potential stem cells from mouse pre-implantation embryos or embryonic stem cells. Nature protocols, 1.
- Kime, C., Rand, T. A., Ivey, K. N., Srivastava, D., Yamanaka, S., & Tomoda, K. (2015). Practical integration‐free episomal methods for generating human induced pluripotent stem cells. Current protocols in human genetics, 87(1), 21-2.
- Takahashi, K., Narita, M., Yokura, M., Ichisaka, T., & Yamanaka, S. (2009). Human induced pluripotent stem cells on autologous feeders. PloS one, 4(12), e8067.
- Park, I. H., & Daley, G. Q. (2009). Human iPS cell derivation/reprogramming. Current protocols in stem cell biology, 8(1), 4A-1.
- Okita, K., Ichisaka, T., & Yamanaka, S. (2007). Generation of germline-competent induced pluripotent stem cells. Nature, 448(7151), 313.
- Takahashi, K., Okita, K., Nakagawa, M., & Yamanaka, S. (2007). Induction of pluripotent stem cells from fibroblast cultures. Nature protocols, 2(12), 3081.
- McMahon, A. P., & Bradley, A. (1990). The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain. Cell, 62(6), 1073-1085.