Newsletter
Cell Line Model Generation
CRISPR Genome Editing for Cell Lines
More than 500 unique cell line models engineered from >100 distinct parental cell lines! CRISPR/Cas9 edited cell line models are great in vitro tools for studying gene function, designing disease models, for drug discovery and high throughput compound screening. At Applied StemCell, we have experience in editing > 100 distinct mammalian cell lines from different species, and have engineered more than 500 cell line models. We can genetically modify hard-to-transfect cells, hematopoietic/ blood lineage cells, slow growing cell lines, adherent/ suspension cells, stem cells and correct mutations in disease cell lines with a >97% success rate.
| Cell Type | Modification Type |
Selected Cell Lines From > 100 distinct parental cell lines engineered A-549 BEAS-2B BT-474 HaCaT HBE Huh7 MCF-10A OCCM-30 RPE-1 SK-MEL-31 Tert-RPE U-2 OS 786-O CHLA-10 A-375 Gist-T1 DLD-1 HCT-116 HEK293 HEK293T HeLa HepG2 4T1 C2C12 cTEC MWCL-1 BCWM-1 H929 Jurkat K562 KHYG-1 LAD2 MM.1s NCI-H929 T2 cells TF-1 HT1080 HT29 KBM-7 KN12-Luc LnCap MDA-MB231 NCI-H2228 RKO TC32 SCC35 SH-SY5Y ES Cells iPSCs |
| Cancer Cell Lines | Knockout Point Mutation Insertion |
|
| Human iPSCs | Knockout Point Mutation Insertion |
|
| Human ES | Insertion | |
| Primate iPSCs | Insertion | |
| Human Primary Cells | Knockout Insertion |
|
| Human Fibroblasts | Point Mutation | |
| Mouse Fibroblasts | Point Mutation | |
| Rat Thyrocytes | Knockout |
Full service cell line editing portfolio includes:
- Targeting vector construction and validation; cell transfection and selection; single cell cloning; expansion and cryopreservation
- Custom edliverables:
- Choice of zygosity: homozygous and/or heterozygous clones
- Choice of silent mutation at targeted site for point mutation insertion
- Dedicated project management; detailed milestone and final reports
Some examples of cell line engineering projects include but are not limited to:
- Knockout modifications;
- Gene knock-in (KI): locus-specific gene insertion; KI into safe harbor locus; gene tagging/ reporter gene insertion
- Gene overexpression
- Conditional / inducible gene expression; Promoter modifications
- Gene editing/ correction, including single base changes
- Gene replacement; gene therapy
- Gene fusion/ translocation
- Removal of viral sequences
- Stable cell lines / immortalization
Applications:
- Recombinant protein production in CHO cells
- Disease model in human cell lines for drug discovery
- Gene therapy in diseased cell line
- Target selection for drug discovery
- High throughput preclinical screening of candidate drugs and toxicity assays
- Custom engineering cell lines for deriving diagnostic reference standards and materials
- Generation of TARGATT™ master cell lines by inserting an attP "docking site" for site-specific gene knock-in
For large DNA/ transgene insertion in cell lines, please refer to the TARGATT™ cell line editing services. The TARGATT™ integrase-based technology offers site-specific gene knock-in and is complementary to CRISPR/Cas9 technology. Both these technologies offers a broader scope for engineering physiologically predictive and advanced cell line models.
We also offer lentivirus-based stable cell line generation for difficult-to-handle cell lines: integration-free lentivirus for CRISPR-lentivirus gene knockout; broad tropism lentiviruses for efficient stable gene knock-in.
Comprehensive Technology Platforms for Gene Editing
|
Methods |
Technical Advantage |
|
TARGATT™ phiC31 integrase |
|
|
CRISPR / Cas9 |
|
Support Materials
Would you like more information on our blood lineage cells editing services? Watch our webinar on CRISPR-based gene editing in blood lineage and blood-derived cell lines, presented by Dr. Jimmy Tai, Senior Scientist and Group Leader at Applied StemCell:
"CRISPR/Cas9 Editing in Blood Lineage Cells: Guidelines to enhance your CRISPR gene editing process"
References
Ordering