Validation Data
Figure 1. mRNA expression of Humanized mice by RT-PCR. Primers P1/2 were specific for mouse sequences and primers P3/4 were specific for human sequences The 487bp product was amplified in HE and WT mice with P1/2 primer pairs. The 372bp product was amplified in HE mice with P3/4 primer pairs. M: 100bp Plus DNA ladder. HE: Heterozygous WT: Wild type
Figure 2. Expression of IL4RA in the Serum of IL4RA-HU Homozygous mice is detected by ELISA. Serum samples of IL4RA HU mice were collected and diluted 10 times before ELISA. HO: Homozygote; WT: Wild type; ND: Not detected
Figure 3. Expression of IL4RA in the Serum of IL4RA-HU Homozygous mice is detected by ELISA. Serum samples of IL4RA HU mice were collected and diluted 10 times before ELISA .
HO: Homozygote; WT: Wild type; ND: Not detected