hIL11(ASHU-204981) was crossed with hIL11RA (ASHU-204982) to generate hIL11/hIL11RA mice.
Validation Data
Figure 1. Detection of IL11 and IL11RA expression in testis, stomach and lung by RT-PCR. Wild type: only one band at 171 bp with primers F1/R1 (mIl11); Homozygous: only one band at 162 bp with primers F2/R2 (hIL11). Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Figure 2. Detection of hIL11 expression in lung homogenate by ELISA. Abbr. HO, homozygous; WT, wild type. Note. hIL11/hIL11RA and C57BL/6 mice were injected with LPS.
Figure 3. Detection of hIL11RA expression in Kidney in C57BL/6 and HO/HO hIL11/hIL11RA knock-in mice by IHC. Abbr. HO/HO, homozygous; WT, wild type. Note. The anti-human IL11RA Antibody cross-reacted with mouse IL11RA and humanized IL11RA.