The endogenous mouse Ifngr1 gene was replaced by human IFNGR1 gene.
Validation Data
Figure 1. Detection of IFNGR1 expression by RT-PCR. Wild type: only one band at 271 bp with primers F2/R2 (mIfngr1); Homozygous: only one band at 296 bp with primers F1/R1 (hIFNGR1); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Figure 2. Validation of hCD119 (IFNGR1) expression in blood by FACS.
Figure 3. Validation of hCD119 (IFNGR1) expression in spleen by FACS.
Figure 4. Validation of hCD119 (IFNGR1) expression in bone marrow by FACS.