PCSK9 - Immune Checkpoint Mouse Models - Mouse Repository - Animal Models - Research

SKU :
ASHU-00075
Catalog # :
ASHU-00075
Availability :
Mouse
Generation Strategy Figure 1. The humanized PCSK9 mice were developed on the C57BL/6 background. The signal peptide of murine Pcsk9 was retained, while the rest of the mouse gene was replaced by its human counterpart. Validation of hPCSK9 Expression in Homozygous Humanized PCSK9 Mice by ELISA Figure 2. Expression of PCSK9 in the serum of humanized PCSK9 homozygous mice is detected by ELISA. A. The expression of human PCSK9 (huPCSK9) was detected in the serum collected from the h .....
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Strain Information
  • Catalog Number ASHU-00075
  • Model Name PCSK9
  • Strain Name C57BL/6J - Pcsk9em2(hPCSK9)/Asc
  • Mutation Type Humanization
  • GENE Synonyms AI415265, AI747682, FH3, HCHOLA3, Narc1, PC9
  • Human Orthologs PCSK9
  • Emsamble ID ENSMUSG00000044254
  • MGI ID 2140260
  • NCBI ID 100102
  • Backgound C57BL/6J
  • Coat Color Black
  • Mutation Type Humanization
Description

Generation Strategy

Landingpage-ASHU-00075-PCSK9-HU-ICPMouse-1.1

Figure 1. The humanized PCSK9 mice were developed on the C57BL/6 background. The signal peptide of murine Pcsk9 was retained, while the rest of the mouse gene was replaced by its human counterpart.

Validation of hPCSK9 Expression in Homozygous Humanized PCSK9 Mice by ELISA

Landingpage-ASHU-00075-PCSK9-HU-ICPMouse-2

Figure 2. Expression of PCSK9 in the serum of humanized PCSK9 homozygous mice is detected by ELISA. A. The expression of human PCSK9 (huPCSK9) was detected in the serum collected from the homozygous, humanized mice. B. The expression of mouse Pcsk9 (muPCSK9) was not detected in the serum collected from homozygous, humanized PCSK9 mice. PCSK9: Proprotein convertase subtilisin/kexin type 9 protein

In Vivo Validation of hPCSK9 Expression in Homozygous Humanized PCSK9 Mice by ELISA

Landingpage-ASHU-00075-PCSK9-HU-ICPMouse-3

Figure 3. Impact of evolocumab treatment on cholesterol (CHO) and low-density lipoprotein cholesterol (LDL-C) levels in the humanized PCSK9 homozygous mice. A single intravenous injection of evolocumab reduced CHO (A) and LDL-C (B) in humanized PCSK9 homozygous mice after a 16-weeks high-fat diet. Average + SD; n = 6 to 7 mice per condition; *p<0.05, **p<0.01; (Student’s T-test).

Research Application
Immunotherapy
Availability
Mouse
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