IL2-HU (ASHU-190048) was crossed with IL2RA-HU (ASHU-190064) to generate humanized IL2 & IL2R mice.
Validation Data
Figure 1. Detection of hIL-2 expression by ELISA in the hIL-2 knock-in mice (Wild type and heterozygous hIL-2 KI mice were administrated with concanavalin).
Figure 2. Intracellular staining of IL-2 by FACS post stimulation with anti-mCD3/anti-mCD28 (Data in partnership with collaborators).
Figure 3. IL-2RA humanized mouse expresses hlL-2RA (hCD25) on naïve Treg in blood (Data in partnership with collaborators).
Figure 4. hlL-2RA is highly expressed on activated Tregs in spleen in the IL-2RA humanized mouse (Data in partnership with collaborators).
Figure 5. hlL-2RA is highly expressed on activated NK cells in spleen in the IL-2RA humanized mouse (Data in partnership with collaborators).