The endogenous mouse Il33 gene was replaced by human IL33 gene.
Validation Data
Figure1. Detection of IL33 expression in lung by RT-PCR. Wild type: only one band at 287 bp with primers F1/R1(mIl33);Homozygous: only one band at 311 bp with primers F2/R2(hIL33); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Figure 2. Detection of hIL33 expression in lung homogenate by ELISA. Abbr. HO, homozygous; HE, heterozygous; WT, wild type. Note. hIL33 and C57BL/6 mice were i.p. injected with LPS (10mg/kg) for 4h.
Figure 3. Detection of mIL33 expression in lung homogenate by ELISA. Abbr. HO, homozygous; HE, heterozygous; WT, wild type. Note. hIL33 and C57BL/6 mice were i.p. injected with LPS (10mg/kg) for 4h.