The endogenous mouse Il12b gene was replaced by human IL12B gene.
Validation Data
Figure 1. Detection of IL12B expression in thymus by RT-PCR. Wild type: only one band at 260 bp with primers F1/R1 (mIl12b); Heterozygous: one band at 260 bp with primers F1/R1 (mIl12b) and one band at 260 bp with primers F2/R2 (hIL12B); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Figure 2. Detection of hIL12B expression in plasma by ELISA. Abbr. HO, homozygous; HE, heterozygous; WT, wild type. Note. hIL12B and C57BL/6 mice were i.p. injected with LPS (10mg/kg) for 2h.
Figure 3. Detection of mIL12B expression in plasma by ELISA. Abbr. HO, homozygous; HE, heterozygous; WT, wild type. Note. hIL12B and C57BL/6 mice were i.p. injected with LPS (10mg/kg) for 2h.