The endogenous mouse Il17a gene was replaced by human IL17A gene.
Validation Data
Figure 1. Detection of IL-17A expression in Large Intestine by RT-PCR. Wild type: only one band at 100 bp with primers F1/R1 (mIl-17a); Homozygous: only one band at 121 bp with primers F2/R2 (hIL-17A); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Figure 2. Expression of human IL17A in cell supernatant derived from splenocytes of IL17A-HU homozygous mice is detected by Elisa.