• Crispr - Mouse Models

Mouse Models

ASC is one of the earliest service providers of CRISPR/Cas9 technology, and has successfully delivered >500 CRISPR mouse models in as little as 3 months. Our animal model portfolio offers competitive pricing and turnaround times for generating CRISPR knockout, conditional knockout, locus-specific/ safe harbor knock-in, controlled gene expression and gene correction, and more.

  • Most up-to-date CRISPR designing strategies and protocols
  • 100% target-site cutting efficiency using optimized, proprietary gRNA validation methods
  • AAALAC-accredited animal facility
  • Animal IP belongs to researchers
  • Project management and scientific support to discuss your project needs and suitable strategic options to fit your budget
  • NEW! Downstream electrophysiology and behavioral assessments for your mouse models
Mouse Models Categories

CRISPR Conditional Knockout
Mouse Models

We can generate conditional knockout mouse models to your specifications. You will have precise control over where or when your gene is knocked-out.

CRISPR Conditional Knockout
Mouse Models

CRISPR Knockout, Knock-in,
Point Mutation Mouse Models

Custom CRISPR mouse model service using advanced design strategies, optimized protocols and validation methods to generate mouse models quickly.

CRISPR Knockout, Knock-in,
Point Mutation Mouse Models

Homologous Recombination Conditional Knockout Mouse Model and Knock-in Mouse Models

Leverage our expertise in knock-in/ knockout mouse model generation, vector designing, ES cell targeting and mouse handling to advance your research.

Homologous Recombination Conditional Knockout Mouse Model and Knock-in Mouse Models

Transgenic Mice Models

Our animal specialists can generate transgenic mouse models using bacterial artificial chromosome (BAC) or random microinjection into the pronucleus.

Transgenic Mice Models

Technical Details

With our expanded facility and newly optimized CRISPR mouse model generation protocols, ASC is offering

  • Steep price-reduction
  • Full money-back guarantee
  • Upgraded and optimized high efficiency protocols
  • Fast turnarounds for F1 mice (germline transmission guaranteed)
  • Mouse models generated in AAALAC-accredited facility and shipped worldwide

In recent years, CRISPR/Cas9 has become a powerful, precision tool to generate genetically engineered mouse or rat models with point mutation(s), small reporter gene insertions, conditional knockout or constitutive knockout. The CRISPR mouse models have paved a novel way for in vivo gene-gene interactions and phenotype association studies, for disease modeling, proof-of-concept gene and cell therapy studies, as well as for preclinical drug efficacy and safety studies.

Applied StemCell (ASC) is one of the first and most experienced provider of the CRISPR-Cas9 Technology. Our expert team of scientists have extensively researched and upgraded the CRISPR system to optimize the efficiency of Cas9 cutting, modification efficiency, and even improve birth rate of mice. With more than 11 years’ experience in mouse model genetic engineering, ASC is a leader in CRISPR mouse model generation service:

Generate a wide variety of mutations to fit your research’s specifications#:

CRISPR MOUSE MODELS

MODEL GENERATION

BENEFITS/ APPLICATIONS

CRISPR Knockout Mouse Models

Constitutive knockout of gene of interest either by frame shift mutation or targeted fragment deletion

  • Study main function of gene/ protein
  • Disease modeling
  • Validate gene function
  • Drug/ target discovery
  • Drug specificity

CRISPR Conditional Knockout (cKO) Mouse Models

Insert LoxP sites to flank gene of interest (floxed allele); the gene of interest is deleted when the cKO mouse is mated with an appropriate Cre-deleter mouse line* which expresses the Cre recombinase under control of a tissue-specific promoter or a mouse line where Cre expression can be induced at a specific developmental stage of the mouse.

 

The cKO mouse models can also be used to generate constitutive germline knockout mice when mated with a

mouse line expressing Cre under control of a ubiquitous promoter.

  • Study gene function in a specific tissue or specific developmental stage
  • Physiologically relevant disease modeling for late-onset or organ/ tissue-specific diseases
  • Overcomes problems such as embryonic lethality, developmental abnormalities and sterility, seen in germline deletion of critical genes1
  • One cKO mouse line enables comparison of gene function in several tissues using hundreds of commercially available Cre-deleter mouse lines*
  • Allows for better understanding of sporadic cancer development, cancer biology and translational oncology2,3

CRISPR Knock-in Mouse Models

Insert a gene of interest or target sequence at a specific genetic locus or a safe harbor locus

 

Reporter genes/ tags, gene/ promoter replacement; humanized gene knock-in; inducible/ conditional expression; gene overexpression mouse models

  • Study promoter regulation, gene expression, stimulus response
  • Modeling human genetics, biology or disease without mouse orthologues
  • Drug development and screening applications
  • Proof-of-concept gene and cell therapy studies
  • Preclinical drug efficacy and safety testing

CRISPR Point Mutation Mouse Models

Substitute one or more nucleotide in the targeted region to result in amino acid change.

  • Study role of nucleotides and amino acids gene and protein function(s) and regulation
  • Clinically relevant mutations for disease modeling
  • Pharmacogenomic screening with cancer relevant mutations

* Cannot find a specific Cre mouse line? We can generate custom Cre mouse line(s) for you using our TARGATT™ site-specific knock-in technology

1 Tratar, U. L., Horvat, S., & Cemazar, M. (2018). Transgenic mouse models in cancer research. Frontiers in oncology, 8.

2 Kersten, K., de Visser, K. E., van Miltenburg, M. H., & Jonkers, J. (2017). Genetically engineered mouse models in oncology research and cancer medicine. EMBO molecular medicine, 9(2), 137-153.

3 Deng, C. X. (2014). Conditional knockout mouse models of cancer. Cold Spring Harbor Protocols, 2014(12), pdb-top074393.


For large fragment knock-in mouse models, our proprietary site-specific, knock-in technology, TARGATT™ has a unique advantage and higher insertion efficiency over CRISPR/Cas9.

Comprehensive Technology Platform for Gene Editing

Methods

Technical Advantage

TARGATT™ phiC31 integrase

  • Site-specific integration (H11 or ROSA26)
  • Works for large DNA Knock-in (~22kb)
  • High efficiency (up to 40%)
  • Insert promoter of choice for gene overexpression/ inducible expression

CRISPR / Cas9

  • High specificity
  • High efficiency in knockout, point mutation, and conditional knockout
  • Ease of use
  • Works for large DNA knock-in (-10kb)

We also offer mouse model generation service using an expanded technology portfolio such as traditional homologous recombination via ESCs, bacterial artificial chromosome and random transgenic technologies. With our expertise in mouse model generation service and various genome editing technologies, we can assure you a custom genetically engineered mouse model perfect for your research needs.

New! Custom In Vivo (Animal Models) Assay Services for downstream evaluation of your animal models. We provide services for in vivo assessments as well as in vitro (end-of-study) evaluations using assays such as electrophysiology, immunohistochemistry, and more.

Publications

CRISPR Knock-in, CRISPR Knockout Mouse

  • Deng, F., He, S., Cui, S., Shi, Y., Tan, Y., Li, Z., ... & Peng, L. (2018). A Molecular Targeted Immunotherapeutic Strategy for Ulcerative Colitis via Dual-Targeting Nanoparticles Delivering miR-146b to Intestinal Macrophages. Journal of Crohn's and Colitis.
  • Jo, S., Fonseca, T. L., Bocco, B. M. D. C., Fernandes, G. W., McAninch, E. A., Bolin, A. P., ... & Németh, D. (2018). Type 2 deiodinase polymorphism causes ER stress and hypothyroidism in the brain. The Journal of Clinical Investigation.
  • Langston, R. G., Rudenko, I. N., Kumaran, R., Hauser, D. N., Kaganovich, A., Ponce, L. B., ... & Beilina, A. (2018). Differences in Stability, Activity and Mutation Effects Between Human and Mouse Leucine-Rich Repeat Kinase 2. Neurochemical research, 1-14.
  • Amara, N., Tholen, M., & Bogyo, M. (2018). Chemical tools for selective activity profiling of endogenously expressed MMP-14 in multicellular models. ACS Chemical Biology. doi: 10.1021/acschembio.8b00562.
  • Allocca, S., Ciano, M., Ciardulli, M. C., D’Ambrosio, C., Scaloni, A., Sarnataro, D., ... & Bonatti, S. (2018). An αB-Crystallin Peptide Rescues Compartmentalization and Trafficking Response to Cu Overload of ATP7B-H1069Q, the Most Frequent Cause of Wilson Disease in the Caucasian Population. International journal of molecular sciences, 19(7).

Homologous Recombination Conditional Knockout Mouse (*cited/published articles)

  • Zhao, M., Tao, F., Venkatraman, A., Li, Z., Smith, S. E., Unruh, J., ... & Marshall, H. (2019). N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells. Cell reports, 26(3), 652-669.
  • Li, C., Zheng, Z., Ha, P., Chen, X., Jiang, W., Sun, S., ... & Chen, E. C. (2018). Neurexin Superfamily Cell Membrane Receptor Contactin‐Associated Protein Like‐4 (Cntnap4) is Involved in Neural EGFL Like 1 (Nell‐1)‐responsive Osteogenesis. Journal of Bone and Mineral Research https://doi.org/10.1002/jbmr.3524.
  • Geraets, R. D., Langin, L. M., Cain, J. T., Parker, C. M., Beraldi, R., Kovacs, A. D., ... & Pearce, D. A. (2017). A tailored mouse model of CLN2 disease: A nonsense mutant for testing personalized therapies. PloS one, 12(5), e0176526
  • Miller, J. N., Kovács, A. D., & Pearce, D. A. (2015). The novel Cln1R151Xmouse model of infantile neuronal ceroid lipofuscinosis (INCL) for testing nonsense suppression therapyHuman Molecular Genetics24(1), 185–196. http://doi.org/10.1093/hmg/ddu428.

For more journal references, please visit our comprehensive list of citations and reference publications.

Have Questions?

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