Mouse Models

 

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Mouse Models, AAALAC

Applied StemCell is a leading CRISPR service company, and we have engineered hundreds of mouse models for academic and corporate researchers successfully. Our animal model portfolio includes constitutive and conditional knockout, site-specific knock-in, large fragment knock-in, gene overexpression and gene correction, to name a few.

  • Leading CRISPR service company for mouse model generation. (Find our name in Nature Biotechnology (2016) 34: 893-894.) 
  • In-licensed CRISPR technology from the Broad Institute.
  • All mice and rats are generated in the USA. We also ship animals internationally.
  • Applied StemCell's animal facility (California) has earned accreditation from AAALAC International (the Association for Assessment and Accreditation of Laboratory Animal Care International).
  • Please feel free to talk with us - we are happy to discuss your projects in detail and provide you with suitable strategic options to fit your budget and research needs.
CRISPR/Cas9 is a powerful new tool we use to generate mouse or rat models with point mutation(s), small reporter gene insertions, conditional or regular knockout(s). For knock-in large DNA fragments in mice, our proprietary site-specific knock-in technology TARGATT™ has a unique advantage and higher insertion efficiency over CRISPR.

Comprehensive Technology Platform for Gene Editing

Methods

Technical Advantage

TARGATT™ phiC31 integrase

  • Site-specific integration (H11 or ROSA26)
  • Works for large DNA Knock-in (~22kb)
  • High efficiency (up to 40%)
  • Insert promoter of choice for gene overexpression/ inducible expression

CRISPR / Cas9

  • High specificity
  • High efficiency in knockout, point mutation, and conditional knockout
  • Ease of use
  • Works for large DNA knock-in (-10kb)

We also offer mouse model generation service using an expanded technology portfolio such as traditional homologous recombination via ESCs, bacterial artificial chromosome and random transgenic technologies. With our expertise in mouse model generation service and various genome editing technologies, we can assure you a custom genetically engineered mouse model perfect for your research needs.

Homologous Recombination

Conditional Knockout & Knock-in Mouse Models

 

Transgenic Mouse Models

Bacterial Artifical Chromosome & Random Microinjection

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