The endogenous mice H2-D1 gene was replaced by human HLA-A2 gene.
Validation Data
Figure 1. Detection of HLA-A2 expression in spleen and intestine by RT-PCR.
Wild type: only one band at 400 bp with primers F1/R1 (mH2-D1); Heterozygous: one band at 400 bp with primers F1/R1 (mH2-D1) and one band at 664 bp with primers F2/R2 (hHLA-A2); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Figure 2. Expression of hHLA-A2 in blood, bone marrow and spleen of HLA-A2 humanized heterozygous mice.
Figure 3. Expression characterization of T, B and NK cells in blood of HLA-A2 humanized heterozygous mice.
Figure 4. Expression characterization of T, B and NK cells in spleen of HLA-A2 humanized heterozygous mice.
Figure 5. Expression characterization of T, B and NK cells in bone marrow of HLA-A2 humanized heterozygous mice.
Figure 6. Expression characterization of neutrophil, monocytes and macrophage cells in blood of HLA-A2 humanized heterozygous mice.
Figure 7. Expression characterization of neutrophil, monocytes and macrophage cells in spleen of HLA-A2 humanized heterozygous mice.
Figure 8. Expression characterization of neutrophil, monocytes and macrophage cells in bone marrow of HLA-A2 humanized heterozygous mice.
The above results were in line with expectations. Wild type mice showed high expression of H-2Db, while HLA-A2 humanized heterozygous mice showed high expression of H-2Db and hHLA-A2. There was no significant difference in the proportion of various immune cells to CD45 positive cells between wild type mice and HLA-A2 humanized mice, indicating that the genetic modification had no significant effect on the proportion of immune cells in mice.