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Strain Information

  • Catalog Number ASHU-190064
  • Model Name IL2RA
  • Strain Name C57BL/6-Il2raem1(hIL2RA)/Asc
  • GENE Synonyms CD25, Il2r, Ly-43
  • Human Orthologs IL2RA
  • Emsamble ID ENSMUSG00000026770
  • MGI ID 96549
  • NCBI ID 16184
  • Background C57BL/6
  • Coat Color Black
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  • Description
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The endogenous mouse Il2ra gene was replaced by human IL2RA gene.

Validation Data


Figure 1. Detection of IL2RA expression by RT-PCR. 

Wild type: only one band at 203 bp with primers F2/R2 (mIl2ra); Homozygous: only one band at 251 bp with primers F1/R1 (hIL2RA);  Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.


Figure 2. Development and validation of hIL2RA knockin mice. (Data in partnership with collaborators)

(A) Targeting strategy for hIL2RA knockin mice development; (B) hIL2A expression on naive Tregcells in blood in homozygous hlL2RA knockin mice; (C) hlL2A expression on naive Treg cells in spleen in homozygous hlL2RA knockin mice. 


Figure 3. Characterization of hIL2RA knockin mice. (Data in partnership with collaborators)

(A) hlL-2RA is highly expressed on activated Tregsin spleen in IL-2RA humanized mouse; (B) hlL-2RA is highly expressed on activated NK cells in spleen in IL-2RA humanized mouse. 


Figure 4. In vivo efficacy study in hIL-2RA knockin mice engrafted with MC38 syngeneic tumor model. (Data in partnership with collaborators)

(A) Tumor growth curves upon treatment; (B) Body weight change upon treatment. 

Immunotherapy, cancer research, drug screening