The endogenous mouse Il2ra gene was replaced by human IL2RA gene.
Validation Data
Figure 1. Detection of IL2RA expression by RT-PCR.
Wild type: only one band at 203 bp with primers F2/R2 (mIl2ra); Homozygous: only one band at 251 bp with primers F1/R1 (hIL2RA); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Figure 2. Development and validation of hIL2RA knockin mice. (Data in partnership with collaborators)
(A) Targeting strategy for hIL2RA knockin mice development; (B) hIL2A expression on naive Tregcells in blood in homozygous hlL2RA knockin mice; (C) hlL2A expression on naive Treg cells in spleen in homozygous hlL2RA knockin mice.
Figure 3. Characterization of hIL2RA knockin mice. (Data in partnership with collaborators)
(A) hlL-2RA is highly expressed on activated Tregsin spleen in IL-2RA humanized mouse; (B) hlL-2RA is highly expressed on activated NK cells in spleen in IL-2RA humanized mouse.
Figure 4. In vivo efficacy study in hIL-2RA knockin mice engrafted with MC38 syngeneic tumor model. (Data in partnership with collaborators)
(A) Tumor growth curves upon treatment; (B) Body weight change upon treatment.