The endogenous mouse Il2rb gene was replaced by human IL2RB gene.
Validation Data
Figure 1. Analysis of IL2RB gene expression in the liver by RT-PCR.
Mouse Il2rb mRNA was detectable by P1/2 (511bp), human IL2RB mRNA was detectable by P1/3(513bp)
M:100bp Plus DNA ladder from Transgen (BM311). The heterozygous KI mouse expresses both human IL2RB and mouse Il2rb in the liver.