The endogenous mouse Il1b gene was replaced by human IL1B gene.
Validation Data
Figure 1. Detection of IL-1B expression in spleen and lung by RT-PCR. Wild type: only one band at 209 bp with primers F1/R1 (mIl-1b); Heterozygous: one band at 209 bp with primers F1/R1 (mIl-1b) and one band at 291 bp with primers F2/R2 (hIL-1B); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Figure 2. Detection of hIL1B expression in plasma by ELISA. Abbr. HO, homozygous; HE, heterozygous; WT, wild type. Note. hIL1B and Balb/c mice were i.p. injected with LPS(10mg/kg) for 6h.