Cell Line Models
Custom Engineering of Your Master Cell Lines! Applied StemCell is one of the longstanding, premier providers of CRISPR/Cas9 service. and a leader in genome engineering technologies. With our experience in having engineered >1300 unique cell line models in >200 distinct mammalian cell lines, for researchers worldwide, we can genetically modify any mammalian cell line using proprietary multi-approach designing and optimized protocols to your specifications:
- Customized deliverables (heterozygous/ homozygous mutations, footprint-free genome editing)
- Variety of mutations: gene knockout/ knock-in (point mutation, reporter knock-in, promoter modifications, conditional gene expression, fusion gene and more
- Generate master cell lines and isogenic cell lines
- Most comprehensive start-to-finish stem cell technology platform
Our custom service also includes high quality service with dedicated project management for timely notifications and reports.
Cell Lines Categories: Cancer, Immortalized, Stem Cell (iPSCs, ESCs) lines; CHO cells; and even Primary cells
Applications: Immuno-oncology, antibody validation, antibody discovery, antibody screening, protein screening/ evolution, isogenic master cell line generation, disease modeling, drug target discovery and toxicity screening.
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Stem Cells - iPSC and ESCs
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Cancer Research
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Antibody Validation
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Antibody Discovery and Screening
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Cell-Based Assays
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Cell-Based Drug Toxicity and Efficacy Testing
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Bioproduction Services- High Yield CHO Cell Lines
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Custom Services
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Fibroblasts Immortalization Service
Applied StemCell offers patient fibroblast immortalization service via the simian virus 40 T antigen. -
Custom Primary Cell Isolation Service
Applied StemCell can isolate primary cells from fresh tissues, including from biopsy samples.
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Cell Line Irradiation Service
Applied StemCell offers gamma-irradiation service for irradiating cell lines (patient-derived, pooled, genome edited/ corrected cell lines) using a Nuclear Regulatory Commission (NRC)-approved Gamma-emission source to irradiate cells at a constant dose rate and to any desired extent of radiation. Irradiated cell line models are novel toolkits for applications such as generation of co-culture models using non-dividing/ feeder cells; to study mechanisms underlying cancer progression; wound healing and inflammatory responses after irradiation; screen drugs for enhancing irradiation sensitivity of tumor cells; and for xenograft tumor models.
Our Cell Line Irradiation Service includes:
- Cell line expansion/ harvesting
- Cell line Irradiation (customized extent of irradiation)
- Cryopreservation
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FFPE Service
Need a Custom FFPE Standards using your own cell line(s)? Custom FFPE Processing of Your Cell Lines: Single or Multi-Spot Capabilities.
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