Cell line genome editing is an extremely valuable and unlimited resource for various applications in cancer research, particularly modeling cancer disease for understanding genetic role in cancer progression, pharmacogenomic studies, drug screening, antibody validation, and more. To date, Applied StemCell has genetically modified > 500 cell lines and uses two gene editing technologies, CRISPR/Cas9 and TARGATT™ site-specific gene integration technologies for cell line model generation.
We can engineer stable cell lines with a variety of genetic modifications perfectly suited for the requirements of your research:
- Gene knockout (KO): frame shift; fragment excision, stop cassette insertion, double KO
- Gene knock-in (KI): point mutation, reporter gene, small and large fragment insertion (locus-specific/ safe harbor locus)
- Controlled gene expression models: gene overexpression; conditional/ inducible gene expression; promoter modifications
- Gene editing/ correction, including single base changes/ point mutation
- Gene replacement; gene therapy
- Gene fusion/ translocation
- Removal of viral sequences
- Master cell line generation
Customizable deliverables: footprint-free genome editing, custom heterozygous/ homozygous clones; point mutations without silent mutations, and more
We are experts in cell line gene editing! We have modified even hard-to-edit cell lines such as Jurkat, B Lymphocytes and other hematopoietic lineage cells using modified CRISPR/Cas9 protocols as well as CRISPR-Lenti techniques. Check out a preliminary list of cell lines in the technical details section or contact us for more details on your cell line of interest.
Cas9 Expressing Cell Lines for an affordable, quick and efficient way to genetically modify cell line and generate isogenic cell lines using 4 widely used cell lines in research, Jurkat, K562, HEK293, and mESC. Also are available, iPSC-reporter Master cell lines for studying development of disease pathogenesis.
Comprehensive Technology Platforms for Gene Editing:
CRISPR / Cas9
CRISPR/Cas9 edited cell line models are great in vitro tools for studying gene function, designing disease models, for drug discovery and high throughput compound screening. At Applied StemCell, we have experience in editing > 100 distinct mammalian cell lines from different species, and have engineered more than 300 cell line models. We can genetically modify hard-to-transfect cells, hematopoietic/ blood lineage cells, slow growing cell lines, adherent/ suspension cells, stem cells and correct mutations in disease cell lines with a >97% success rate.
|Cell Type||Modification Type||
Selected Cell Lines From > 100 distinct parental cell lines engineered
A-549 BEAS-2B BT-474 HaCaT HBE Huh7
MCF-10A OCCM-30 RPE-1 SK-MEL-31 Tert-RPE
U-2 OS 786-O CHLA-10 A-375 Gist-T1 DLD-1
HCT-116 HEK293 HEK293T HeLa HepG2 4T1
C2C12 cTEC MWCL-1 BCWM-1 H929 Jurkat
K562 KHYG-1 LAD2 MM.1s NCI-H929 T2 cells
TF-1 HT1080 HT29 KBM-7 KN12-Luc LnCap
MDA-MB231 NCI-H2228 RKO TC32 SCC35
SH-SY5Y ES Cells iPSCs
|Cancer Cell Lines||Knockout
|Human Primary Cells||Knockout
|Human Fibroblasts||Point Mutation|
|Mouse Fibroblasts||Point Mutation|
|Catalog ID#||Product Name||Price|