Cancer and Immortalized Cell Lines
Gene editing in cancer and immortalized cell lines provides an extremely useful toolset for various biomedical applications including understanding role of genes in cellular processes and disease mechanisms such as cancer, drug target discovery and screening, antibody validation, and more. Applied StemCell has genetically modified > 300 cell lines using two gene editing technologies, CRISPR/Cas9 and TARGATT™ site-specific gene integration technologies.
We can generate cell lines with a variety of genetic modifications:
- Gene knockout
- Gene knock-in (KI): locus-specific gene insertion; KI into safe harbor locus; gene tagging/ reporter gene insertion
- Master cell line generation
- Gene overexpression
- Conditional / inducible gene expression
- Promoter modifications
- Gene editing/ correction, including single base changes
- Gene replacement; gene therapy
- Gene fusion/ translocation
- Removal of viral sequences
- Stable cell lines / immortalization Customizable deliverables: custom heterozygous/ homozygous clones; point mutations without silent mutations, and more
We are experts in cell line gene editing! We have modified even hard-to-transfect or edit cell lines such as Jurkat, B Lymphocytes and other hematopoietic lineage cells using modified CRISPR/Cas9 protocols as well as CRISPR-Lenti techniques. Check out a preliminary list of cell lines in the technical details section or contact us for more details on your cell line of interest.
Applications: Disease modeling and correction, studies to elucidate cellular and genetic mechanisms, drug target discovery and screening, antibody validation and tissue engineering.
Cas9 EXPRESSING CELL LINES FOR ISOGENIC CELL LINE GENERATION: Applied StemCell catalog now includes Cas9 expressing cell lines for an quick and efficient way to genetically modify 4 wide used cell lines: Jurkat, K562, HEK293, and mESC. Generate your own isogenic cell lines with these Cas9 expressing cells quickly, efficientlt and affordably.
Comprehensive Technology Platforms for Gene Editing
CRISPR / Cas9
CRISPR/Cas9 edited cell line models are great in vitro tools for studying gene function, designing disease models, for drug discovery and high throughput compound screening. At Applied StemCell, we have experience in editing > 100 distinct mammalian cell lines from different species, and have engineered more than 300 cell line models. We can genetically modify hard-to-transfect cells, hematopoietic/ blood lineage cells, slow growing cell lines, adherent/ suspension cells, stem cells and correct mutations in disease cell lines with a >97% success rate.
|Cell Type||Modification Type||
Selected Cell Lines From > 100 distinct parental cell lines engineered
A-549 BEAS-2B BT-474 HaCaT HBE Huh7
MCF-10A OCCM-30 RPE-1 SK-MEL-31 Tert-RPE
U-2 OS 786-O CHLA-10 A-375 Gist-T1 DLD-1
HCT-116 HEK293 HEK293T HeLa HepG2 4T1
C2C12 cTEC MWCL-1 BCWM-1 H929 Jurkat
K562 KHYG-1 LAD2 MM.1s NCI-H929 T2 cells
TF-1 HT1080 HT29 KBM-7 KN12-Luc LnCap
MDA-MB231 NCI-H2228 RKO TC32 SCC35
SH-SY5Y ES Cells iPSCs
|Cancer Cell Lines||Knockout
|Human Primary Cells||Knockout
|Human Fibroblasts||Point Mutation|
|Mouse Fibroblasts||Point Mutation|