Cancer Research

 

 button-crispr-cell-line-model-generation          button-targatt-cell-models

Cell line genome editing is an extremely valuable and unlimited resource for various applications in cancer research, particularly modeling cancer disease for understanding genetic role in cancer progression, pharmacogenomic studies, drug screening, antibody validation, and more. To date, Applied StemCell has genetically modified > 500 cell lines and uses two gene editing technologies, CRISPR/Cas9 and TARGATT™ site-specific gene integration technologies for cell line model generation.

We can engineer stable cell lines with a variety of genetic modifications perfectly suited for the requirements of your research:

  • Gene knockout (KO): frame shift; fragment excision, stop cassette insertion, double KO
  • Gene knock-in (KI): point mutation, reporter gene, small and large fragment insertion (locus-specific/ safe harbor locus)
  • Controlled gene expression models: gene overexpression; conditional/ inducible gene expression; promoter modifications
  • Gene editing/ correction, including single base changes/ point mutation
  • Gene replacement; gene therapy
  • Gene fusion/ translocation
  • Removal of viral sequences
  • Master cell line generation

Customizable deliverables: footprint-free genome editing, custom heterozygous/ homozygous clones; point mutations without silent mutations, and more

We are experts in cell line gene editing! We have modified even hard-to-edit cell lines such as Jurkat, B Lymphocytes and other hematopoietic lineage cells using modified CRISPR/Cas9 protocols as well as CRISPR-Lenti techniques. Check out a preliminary list of cell lines in the technical details section or contact us for more details on your cell line of interest.


Genetically Modified Cell Lines/ Isogenic Cell Lines:

Cas9 Expressing Cell Lines for an affordable, quick and efficient way to genetically modify cell line and generate isogenic cell lines using 4 widely used cell lines in research, Jurkat, K562, HEK293, and mESC. Also are available, iPSC-reporter Master cell lines for studying development of disease pathogenesis.


Comprehensive Technology Platforms for Gene Editing:

Methods

Technical Advantage

TARGATT™ 

phiC31 integrase

  • Site specific integration in "Safe harbor locus"(ROSA26)
  • High efficiency (up to 40%)
  • Works for large fragment knock-in(-22kb)
  • Insert promoter of choice for gene: overexpression and inducible expression
  • Works independent of cell division

CRISPR / Cas9

  • High specificity
  • High frequency for Knockout, point mutation
  • Large DNA knock-in up to 10kb
  • Generate homozygous or heterozygous modified cell lines

 

Technical Details

CRISPR/Cas9 edited cell line models are great in vitro tools for studying gene function, designing disease models, for drug discovery and high throughput compound screening. At Applied StemCell, we have experience in editing > 100 distinct mammalian cell lines from different species, and have engineered more than 300 cell line models. We can genetically modify hard-to-transfect cells, hematopoietic/ blood lineage cells, slow growing cell lines, adherent/ suspension cells, stem cells and correct mutations in disease cell lines with a >97% success rate.

Cell Type Modification Type

Selected Cell Lines From > 100 distinct parental cell lines engineered

A-549    BEAS-2B    BT-474   HaCaT   HBE   Huh7  

MCF-10A    OCCM-30    RPE-1   SK-MEL-31   Tert-RPE    

U-2 OS   786-O    CHLA-10   A-375   Gist-T1   DLD-1  

HCT-116    HEK293   HEK293T    HeLa   HepG2   4T1  

C2C12     cTEC    MWCL-1   BCWM-1    H929    Jurkat    

K562    KHYG-1    LAD2    MM.1s     NCI-H929     T2 cells    

TF-1     HT1080    HT29     KBM-7    KN12-Luc     LnCap    

MDA-MB231    NCI-H2228     RKO     TC32    SCC35    

SH-SY5Y    ES Cells    iPSCs  

Cancer Cell Lines Knockout
Point Mutation
Insertion
Human iPSCs Knockout
Point Mutation
Insertion
Human ES Insertion
Primate iPSCs Insertion
Human Primary Cells Knockout
Insertion
Human Fibroblasts Point Mutation
Mouse Fibroblasts Point Mutation
Rat Thyrocytes Knockout
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