Bioproduction Services- High Yield CHO Cells

TARGATT™ Chinese Hamster Ovary (CHO) Cells for Recombinant Proteins and Antibody Bioproduction

The traditional CHO antibody production method using random gene insertion and forced amplification of transgenes adversely affects bioproduction levels due to factors position effects, repeat-induced gene silencing and genomic instability, altered regulation or interruption of endogenous genes. To overcome these drawbacks, CHO bioproduction technology needs to focus on transgene integration at a defined genomic locus with guaranteed high levels of expression, high integration efficiency, and easy, reproducible screening of clones.. In addition, it is crucial that the cell line is viable and stably reproduced.

Applied StemCell’s (ASC) proprietary, site-specific TARGATT™ technology has met and exceeded these goals for use in CHO cell lines. ASC has created Master TARGATT™ CHO cell lines for the rapid creation of transgenic lines for high level protein and antibody expression and is proud to announce the launch of our CHO Bioproduction cell line technology.

Benefits

  • Genomically well-defined system
  • Stable integration of expression cassette
  • Safe-harbor locus minimizes chances of transgene silencing

Key points:

  • Ablility to achieve > 1 g/l antibody bioproduction using a single site insertion with little optimization
  • Identification of the ASC-X locus has allowed for a further, > 2.5-fold increase in protein expression levels 

Our TARGATT™ technology improves CHO antibody production capability by reducing cost and improving manufacturing feasibility for companies and projects of all sizes.Please let us know if you have questions about your CHO bioproduction project.

Features

TARGATT™ Site-Specific Insertion

Traditional Random Gene Insertion

Safe Genomic Locus

Yes

No; random

Protein Yield

High

Varies

Gene Insertion Efficiency

High

Low

Reproducibility

Yes; for any protein

No; different from one protein to another

Copy # of Inserted Gene

Single copy

Multiple copies

Stable

Yes

No; Chromosome rearrangement

Gene Silencing

No

Yes


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Technical Details

Technical Details for Bioproduction in TARGATT™ CHO Cells

Antibody Production in TARGATT™ CHO-H11 cells

TECHNICAL-bioproduction-CHOcells-1

Figure 1. Expression of antibodies Ab1 and Ab2 in the H11 genomic hotspot locus of the TARGATT™ CHO-H11 cells yielded ~1g/L of each protein.

Optimizing Docking Site Location in TARGATT™ CHO Cell Lines

TECHNICAL-bioproduction-CHOcells-2

Figure 2. Expression of green fluorescent protein (GFP) in various genomic hotspot loci in TARGATT™ CHO cells. TARGATT™ CHO master cell lines were engineered with attP docking site in the well-characterized H11 locus and 4 different proprietary loci, ASC1, ASC2, ASC3, and ASC4. The GFP reporter gene was inserted into each cell line and its expression from each locus was measured using FACS analysis. Reporter expression in the ASC2 locus was 2.5-fold higher than the expression in the H11 locus. The estimated protein yield from the TARGATT™ CHO-ASC2 Master cell line is ~2.5 g/L.

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