CRISPR Custom Point Mutation iPSC Generation Service [ASC-6013-3] : AppliedStemCell eCommerce Platform, Advance stem cell innovation

Point Mutation Cell Lines mimic mutations seen in clinical specimens and are important in vitro disease models to understand role of genes in diseases (immuno-oncology, autoimmune diseases) as well as for drug discovery and immunotherapy development.

Leverage Applied StemCell unique expertise in CRISPR iPSC/ ES Cell Genome Editing Service to generate a point mutation iPSC line in your gene of interest:

• Send us your healthy/ patient iPSC or ESC lines; OR
• Choose from our well-characterized control “master” iPSC lines

Also available, downstream iPSC differentiation and characterization services.

• Optimized CRISPR protocols for high success rate
• Homozygous/ heterozygous point mutation clones
• Footprint-free, feeder-free transfection and iPSC culture protocols
• Isogenic control lines for reliable experiments

Workflow includes:

• Cell line evaluation
• gRNA design, CRISPR vector and donor DNA construction, and reagent validation
• Transfection of targeting vectors and optimization
• Screening for single cell clones and clone confirmation
• Cell expansion and cryopreservation

Deliverables:

• Two (2) clones for targeted point mutation, with two (2) vials of each clone at 1 x 10^6 cells/vial.
• Project Milestones Reports, as well as a Final Report containing details of targeting design, experimental, and genotyping results.

Timeline: 3-4 months

Applications:

• Physiologically relevant disease models for hard-to-model diseases (Ex. ALS, muscular dystrophy, Parkinson's disease, Alzheimer's)
• Differentiate to study mutations in different tissue lineages with an isogenic panel of cell line models
• Ideal for target drug discovery, drug and toxicity screening