CRISPR (Cas9) Knockout iPSC Service

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Knockout iPS Cell Generation Service Details

Applied StemCell(ASC) is a leader in both CRISPR genome editing and iPSC technologies. With >500 unique iPS cell line models generated to date and a >98% success rate, leverage our unique expertise to knockout your gene of interest in your patient iPSCs or our control lines.

Other features include:

  • Frameshift or target exon deletion; Large fragment deletion (>10 kb)
  • Homozygous (or heterozygous) knockout clones
  • Footprint-free, feeder-free transfection, and iPSC culture protocols
  • Isogenic control lines for reliable experiments
  • Fully customizable

Standard Packaging:

Standard Deliverables:

  • Two (2) clones that are homozygous (or heterozygous) for the targeted null allele, with two (2) vials of each clone at 1 x 10^6 cells/vial.
  • Project Milestones Reports, as well as a Final Report containing details of targeting design, experimental, and genotyping results.

Standard Workflow & Timeline:

  • Cell line evaluation
  • gRNA design, construction, and validation
  • Transfection and optimization
  • Screening for single-cell clones and clone confirmation
  • Cell expansion and cryopreservation

iPSC Genome Editing Service Workflow - Knockout iPS Cells

6-8 weeks (w/ ASC's control line)
3-4 months (w/ your iPSCs)

Required Starting Material & Information


  • Physiologically relevant disease models for hard-to-model diseases (Ex. ALS, muscular dystrophy, Parkinson's disease, Alzheimer's)
  • Differentiate to study mutations in different tissue lineages with an isogenic panel of cell line models
  • Ideal for target drug discovery, drug and toxicity screening

Applied StemCell can also provide expanded characterization and differentiation to somatic lineages such as NK cells, T cells, astrocytesretinal pigment epithelium (RPE), cardiomyocytes, hepatocytes, and more. ASC invites you to look through our new GMP-grade iPSC service page to learn about how we can help in furthering your research in a GMP setting.