Transgenic Rat Model Generation Service Using BAC

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Transgenic Rat Model Generation Service Using BAC

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  • Fully Customizable - Inquire

Standard Deliverables:

  • At least 2 founders (or germline transmitted F1s) with confirmed gene knockout
  • Milestone update/report; final report with a detailed description of each procedure, including targeting vector design, construction and validation, transfection condition, genotyping strategy, and results

Standard Workflow and Timeline:

 Service Time
1.  DNA BAC vector design, cloning, and recombineering  6-8weeks
Design, strategy, and BAC recombineering  
Enzyme digestion identification and sequencing to confirm
Purification of BAC DNA for microinjection
2. DNA pronuclear microinjection and implantation of embryos
 2 month
Pronuclear microinjection and embryo implantation
 7-10 weeks
3. Animal Care, Housing, and Genotyping Founders
1 month
Deliverable: Confirmed positive founder(s)

For the knockout of an essential gene, ASC offers PrimeCKO™ Rat Knockout Mouse Model Generation service.

All our animals are housed in a facility that is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International, has an Assurance on file with the Office of Laboratory Animal Welfare (OLAW), and is a registered research facility with the U.S. Department of Agriculture.