Applied StemCell, Inc. provides Human Induced Pluripotent Stem (hiPS) Cells (Research Grade) that were generated from CD34+ umbilical cord blood cells using an epsiomal reprogramming method. The ASE-9250 research grade hiPSC line is from a male donor and has been fully characterized. This includes expression of pluripotency markers (Oct4, Sox2, Tra-1-60, and Tra-1-81; Figure 2), alkaline phosphatase (AP) staining (Figure 3), STR analysis (Figure 5), and the ability to differentiate into the three germ layers (Figure 4). The ASE-9250 human iPSC line can be used for CRISPR/Cas9 genome editing and differentiation to somatic lineages in vitro.
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Reprogramming Method |
Episomal
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Tissue |
CD34+ Umbilical Cord Blood Cells
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Sex |
Male
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Clinical Information |
Normal
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Quantity |
1 x 106 cells/vial
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Shipping |
Dry ice
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Storage and Stability |
Store in liquid nitrogen freezer immediately upon receipt. This product is stable for at least 6 months from the date of receiving when stored as directed.
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Quality Control |
Each lot of human iPS cells has been tested for viability following recovery from cryopreservation. In addition, the hiPSCs have also been tested for functional pluripotency (formation of the three germ layers), expression of pluripotency markers (Oct4, Sox2, Tra-1-60, and Tra-1-81), presence of alkaline phosphatase (AP), STR, sterility, and for the absence of mycoplasma and pathogens (CofA available upon request).
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