Genetically Modified Cell Lines / Isogenic Cell Lines
Isogenic Cell Lines for Drug Dicovery: Isogenic cell lines represent a unique toolset for studying the impact of genotype on cellular phenotype.
Isogenic Cell Lines for Drug Dicovery: Isogenic cell lines represent a unique toolset for studying the impact of genotype on cellular phenotype. An isogenic cell line is a cell line that has been engineered from a parental line through the introduction of a targeted gene mutation. In doing so, the parental cell line inherently becomes a control line to which the engineered line can be referenced. Isogenic cell line pairs can be incredibly useful as tools for studying cellular biology, such as the impact of genotype on cellular phenotype, as well as for parallel, high throughput screening to enable the discovery of therapeutic compounds that exhibit genotype-specific toxicity. Applied StemCell offers a number of homozygous isogenic cell lines.
For genome engineering applications, we also offer cell lines that have been modified to enable more efficient genome editing, such as our Cas9-expressing Jurkat line, and hiPSC TARGATT™ Master line.
In addition to our off-the-shelf products, we offers custom cell line engineering services for the generation of knock-out, point mutation, knock-in, inducible, and reporter cell lines.
Cell Lines expressing the CRISPR Cas9 nuclease enable you to carry out CRISPR genome editing applications including cell based assays and drug discovery.
ASC’s Gene Edited iPS Cell Line catalog includes isogenic panels of reporter knock-in iPSC lines from a fully characterized control/ master iPSC (ASE-9109). These reporter iPSC lines are fully characterized and are ideal for studying neurological mechanisms and drug testing: (1) Neuronal lineage-specific reporter gene knock-in lines; (2) Safe harbor locus reporter gene knock-in iPS lines.
Isogenic Gene knockout (KO) iPSCs engineered from control iPSC line (ASE-9109), that are fully characterized and are ideal for neurological disease modeling.