• Stem Cell

Stem Cell

Applied StemCell has a comprehensive catalog of products for every aspect of your stem cell research:

  • Ready-to-use induced pluripotent stem cell (iPSC) and embryonic stem cell (ESC) lines.
  • iPSC-derived differentiated neural lineage cells and cardiomyocytes.
  • For the more hands-on researcher, we offer protocols and kits for iPSC generation to generate your own iPSCs.
  • To help you maintain your stem cells, we also offer high quality and thoroughly tested mouse embryonic fibroblasts (MEF) feeder cells, and stem cell-grade “pre-validated“ FBS and conditioned ESC/iPSC Medium.
Stem Cell Categories

iPSC & ESC Lines

Ready-to-use, fully characterized human iPSCs from different sources and clinical conditions, iPSC from other mammalian species, and mouse ESC lines.

iPSC & ESC Lines

iPSC Differentiated Cells

We offer fully-characterized iPSC-differentiated progenitors and cell lines: Neural stem cells (NSC), neurons, astrocytes, and cardiomyocytes.

iPSC Differentiated Cells

Gene Edited iPSC Lines

Control iPSC-derived isogenic panels of neuronal gene knockout, lineage-specific reporter knock-in, and safe harbor locus reporter knock-in iPSC lines.

Gene Edited iPSC Lines

Stem Cell Culture:
MEF Feeder Cells, 3D Media, FBS

Validated, high quality MEF feeders, and ESC-Sure™ FBS and conditioned serum/feeder-free (SFFM) medium for robust growth of your stem cells.

Stem Cell Culture:
MEF Feeder Cells, 3D Media, FBS

iPSC Generation
Kit

Reprogram your own iPSCs from your somatic cells using either our Human EZ- iPSC Retroviral or Episomal Generation kits.

iPSC Generation
Kit

Products and Services
Catalog ID#Product Name SizePriceQTY
$5,000.00
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$5,000.00
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$104.00
$210.00
$189.00
$95.00
$95.00
$95.00
$100.00
$950.00
$1,500.00
$380.00
$3,750.00
$515.00
$268.00
$268.00
$48.00
$100.00
$1,500.00
$595.00
$1,150.00
$650.00
$800.00
$800.00
$200.00
$126.00
$126.00
$48.00
$48.00
$226.00
$835.00
$940.00
$100.00
$1,500.00
$650.00
$800.00
$800.00
$200.00

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Publications

iPSC/ESCs

ASE-9109: Normal iPSC differentiation to cardiomyocytes

  • Kavyasudha C., Macrin D., ArulJothi K.N., Joseph J.P., Harishankar M.K., Devi A. (2018) Clinical Applications of Induced Pluripotent Stem Cells – Stato Attuale. In: Advances in Experimental Medicine and Biology. Springer, New York, NY.https://doi.org/10.1007/5584_2018_173.

  • Lin, Y., Linask, K. L., Mallon, B., Johnson, K., Klein, M., Beers, J., ... & Zou, J. (2017). Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined AlbuminFree Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem cells translational medicine6(2), 527-538.

ASE-9208: Sporadic Parkinson’s disease line

  • Hsieh, C. H., Shaltouki, A., Gonzalez, A. E., da Cruz, A. B., Burbulla, L. F., Lawrence, E. S., ... & Wang, X. (2016). Functional impairment in Miro degradation and mitophagy is a shared feature in familial and sporadic Parkinson’s disease. Cell Stem Cell19(6), 709-724.

Human iPSCs: iPSCs from ALS8 patient and non-carrier siblings

  • Mitne-Neto, M., Machado-Costa, M., Marchetto, M. C., Bengtson, M. H., Joazeiro, C. A., Tsuda, H., ... & Muotri, A. R. (2011). Downregulation of VAPB expression in motor neurons derived from induced pluripotent stem cells of ALS8 patients. Human molecular genetics20(18), 3642-3652.

iPSC-derived cardiomyocyte

  • Daily, N. J., et al. (2017). High-Throughput Phenotyping of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Neurons Using Electric Field Stimulation and High-Speed Fluorescence Imaging. ASSAY and Drug Development Technologies. 15(4): 178-188. https://doi.org/10.1089/adt.2017.781

  • Daily, N. J., Santos, R., Vecchi, J., Kemanli, P., & Wakatsuki, T. (2017). Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools. Journal of evolving stem cell research, 1(2), 1.

  • Daily, N. J., et al. (2015). Journal of Bioengineering & Biomedical Science, 2015.

Mouse/ Rat/ Pig/ Guinea Pig

MyEZGel™ 3D-Matrix

  • Li, L., & LaBarbera, D. V. (2017). 3D High-Content Screening of Organoids for Drug Discovery. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. 388-415. doi.org/10.1016/B978-0-12-409547-2.12329-7

MEF Feeder Cells

DR4 MEF Feeder Cells (*cited/published articles)

  • Fogarty, N. M., McCarthy, A., Snijders, K. E., Powell, B. E., Kubikova, N., Blakeley, P., ... & Maciulyte, V. (2017). Genome editing reveals a role for OCT4 in human embryogenesis. Nature, 550(7674), 67-73.

  • Molokanova, O., Schönig, K., Weng, S. Y., Wang, X., Bros, M., Diken, M., ... & Eshkind, L. (2017). Inducible knockdown of procollagen I protects mice from liver fibrosis and leads to dysregulated matrix genes and attenuated inflammation. Matrix Biologyhttps://doi.org/10.1016/j.matbio.2017.11.002.

  • Marttila, S. (2017). Establishment and characterisation of new human induced pluripotent stem cell lines and cardiomyocyte differentiation: a comparative view. Master’s Thesis, University of Tampere, May 2017.

  • Honda, A., Kawano, Y., Izu, H., Choijookhuu, N., Honsho, K., Nakamura, T., ... & Sankai, T. (2017). Discrimination of stem cell status after subjecting cynomolgus monkey pluripotent stem cells to naive conversion. Scientific reports7, 45285.

  • Friedel, T., Jung-Klawitter, S., Sebe, A., Schenk, F., Modlich, U., Ivics, Z., ... & Schneider, I. C. (2016). CD30 Receptor-Targeted Lentiviral Vectors for Human Induced Pluripotent Stem Cell-Specific Gene Modification. Stem cells and development25(9), 729-739.

  • Ludwig, M., Kitzenberg, D., & Chick, W. S. (2015). Forward Genetic Approach to Uncover Stress Resistance Genes in Mice—A High-throughput Screen in ES Cells. Journal of visualized experiments: JoVE, (105).

  • Neri, T., Muggeo, S., Paulis, M., Caldana, M. E., Crisafulli, L., Strina, D., ... & Scanziani, E. (2015). Targeted gene correction in osteopetrotic-induced pluripotent stem cells for the generation of functional Osteoclasts. Stem cell reports5(4), 558-568.

  • Kraus, P., Sivakamasundari, V., Xing, X., & Lufkin, T. (2014). Generating mouse lines for lineage tracing and knockout studies. In Mouse Genetics(pp. 37-62). Humana Press, New York, NY.

  • Zhu, F., Gamboa, M., Farruggio, A. P., Hippenmeyer, S., Tasic, B., Schüle, B., ... & Calos, M. P. (2013). DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Nucleic acids research42(5), e34-e34.

  • Ivics, Z., Izsvák, Z., Chapman, K. M., & Hamra, F. K. (2011). Sleeping Beauty transposon mutagenesis of the rat genome in spermatogonial stem cells. Methods53(4), 356-365.

  • Ivics, Z., Izsvák, Z., Medrano, G., Chapman, K. M., & Hamra, F. K. (2011). Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cells. Nature protocols6(10), 1521.

  • Pan, Y. (2011). Culturing of C57BL/6 Mouse Embryonic Stem (ES) Cell Line. Bio-protocol Bio101: e142. DOI: 10.21769/BioProtoc.142.

  • Chapman, K. M., Saidley-Alsaadi, D., Syvyk, A. E., Shirley, J. R., Thompson, L. M., & Hamra, F. K. (2011). Rat spermatogonial stem cell-mediated gene transfer. In Advanced Protocols for Animal Transgenesis (pp. 237-266). Springer, Berlin, Heidelberg.

CF-1 MEF Feeder Cells (*cited/published articles)

Neo-resistant MEF Feeder Cells (*cited/published articles)

SNL 76/7 (STO Cell Line)

ESC-Sure™ FBS

  • Hodges, H. C., Stanton, B. Z., Cermakova, K., Chang, C. Y., Miller, E. L., Kirkland, J. G., ... & Crabtree, G. R. (2017). Dominant-negative SMARCA4 mutants alter the accessibility landscape of tissue-unrestricted enhancers. Nature Structural & Molecular Biology, 1.

  • Braun, S. M. G., Kirkland, J. G., Chory, E. J., Husmann, D., Calarco, J. P., & Crabtree, G. R. (2017). Rapid and reversible epigenome editing by endogenous chromatin regulators. Nature Communications, 8, 560.http://doi.org/10.1038/s41467-017-00644-y.s

  • Dykhuizen, E. C., Carmody, L. C., & Tolliday, N. J. (2017). High-Throughput Screening of Small Molecule Transcriptional Regulators in Embryonic Stem Cells Using qRT-PCR. In Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases (pp. 81-95). Humana Press, New York, NY.

  • Stanton, B. Z., Hodges, C., Calarco, J. P., Braun, S. M. G., Ku, W. L., Kadoch, C., … Crabtree, G. R. (2017). SMARCA4 ATPase mutations disrupt direct eviction of PRC1 from chromatin. Nature Genetics49(2), 282–288. http://doi.org/10.1038/ng.3735

  • Beske, P. H., Bradford, A. B., Grynovicki, J. O., Glotfelty, E. J., Hoffman, K. M., Hubbard, K. S., ... & McNutt, P. M. (2015). Botulinum and tetanus neurotoxin-induced blockade of synaptic transmission in networked cultures of human and rodent neurons. Toxicological Sciences149(2), 503-515. doi: 10.1093/toxsci/kfv254

  • Miljan, E. (2015) The Business of Stem Cell Research Tools, in Stem Cells in Regenerative Medicine: Science, regulation and business strategies (eds A. A. Vertès, N. Qureshi, A. I. Caplan and L. E. Babiss), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9781118846193.ch8.

  • Hubbard, K., Beske, P., Lyman, M., & McNutt, P. (2015). Functional evaluation of biological neurotoxins in networked cultures of stem cell-derived central nervous system neurons. Journal of visualized experiments: JoVE, (96).

  • Stanford Medicine Transgenic Research center (http://med.stanford.edu/transgenic/esmeflif.html)

  • Hathaway, N. A., Bell, O., Hodges, C., Miller, E. L., Neel, D. S., & Crabtree, G. R. (2012). Dynamics and memory of heterochromatin in living cells. Cell149(7), 1447-1460.

Medium

ESC-Sure™ Serum-/Feeder- Free hESC/iPSC Culture Medium (SFFM)-  *cited/published articles

  • *Chai, S., Wan, X., Ramirez-Navarro, A., Tesar, P. J., Kaufman, E. S., Ficker, E., ... & Deschênes, I. (2018). Physiological genomics identifies genetic modifiers of long QT syndrome type 2 severity. The Journal of clinical investigation128(3).

  • Szabo, AZ., et al. (2013) Stem Cells. 31(4):786-99

ESC-Sure™ mESC Complete Medium (*cited/published articles)

  • Okada, A., et al. (2016) Embryonic Stem Cell Protocols, 181-195.

ESC-Sure™ Basal Medium Mouse DMEM

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!