• Bioproduction

Bioproduction

The traditional CHO antibody production and animal bioproduction methods are inefficient because they require random gene insertion and forced amplification of transgenes. Applied StemCell has addressed these problems by utilizing our expertise in cell line and animal model engineering with our proprietary TARGATT™ technology to develop two bioproduction methods: TARGATT™ CHO Master Cell Lines and Transgenic TARGATT™ Rabbits. Both methods offer low-cost, efficiency, consistent protein expression and high protein yield that is easily scalable for large scale bioproduction.

Bioproduction Categories

CHO Cells

Site-specific transgene knock-in using TARGATT CHO Master Cell Lines for the creation of high protein/antibody expression in bioproduction cells.

CHO Cells

Transgenic Animals - Rabbit

Applied StemCell's animal bioproduction platform uses transgenic TARGATT™ Rabbits to consistently express recombinant proteins at a very high yield.

Transgenic Animals - Rabbit

Products and Services

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Application Notes

CHO Bioproduction

CHO Bioproduction Service

  • Safe-Harbor Locus: H11 (non-optimized locus)
  • Proteins Expressed: Two therapeutic antibodies, Ab1 and Ab2
  • Copies Inserted: 1 (single copy)
  • Production Parameters:
    • 17-day fed-batch shake-flask
    • Bulk enriched pools (no single-cell cloning)
  • Yield: ~1 g/l

Biopharming

  • Bioproduction in rabbit milk
  • TdTomato proof-of-concept
    • Tdtomato Molecular weight = 56 kD
    • Factor VII molecular weight = 52 kD
  • F1 heterozygous rabbit (1 copy)
  • Yield: 18g/l in homozygous rabbits
Technical Details

Applied StemCell Bioproduction Details

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