• Stem Cell Services

Stem Cell Services

Accelerate your research and discoveries by leveraging our extensive expertise in stem cell technology! We can provide physiologically relevant in vitro models derived from Pluripotent Stem Cells (PSC) for more predictive models of human disease and biology. ASC offers a comprehensive custom service platform for every aspect of stem cell-based research: iPSC generation/ ESC derivation, CRISPR/Cas9 genome editing and cell line model generation; differentiation to somatic lineage of choice, downstream assays to validate your cell line and for drug discovery and screening.

Stem Cell Services Categories

iPSC Generation
from Patient Samples

Custom iPSC generation from healthy/ disease PBMCs or fibroblasts using episomal, mRNA, other integration-free or retroviral reprogramming methods.

iPSC Generation
from Patient Samples

Stem Cell
Genome Editing

Custom mutation engineering & correction in control orpatient iPSCs using CRISPR/Cas9 for predictive in vitromodeling of human biology/disease.

Stem Cell
Genome Editing

Safe Harbor Locus Master
iPSC Genome Editing Service

Custom isogenic and reporter iPSC lines generated using our site-specific TARGATT™ gene integration technology and TARGATT™ iPSC Master cell line.

Safe Harbor Locus Master
iPSC Genome Editing Service

Neural Stem Cell
Differentiation

Service for ESC / iPSC differentiation into Neural Stem Cells (NSCs) , Motor Neurons and Glial Cells (Astrocytes and Oligodendrocytes).

Neural Stem Cell
Differentiation

Cardiomyocytes
Differentiation

Comprehensive service to differentiate iPSCs into high purity, functional cardiomyocytes from healthy/ disease samples.

Cardiomyocytes
Differentiation

Hepatocytes
Differentiation

Custom differentiation of iPSCs to mature and robust population of ready-to-use hepatocytes.

Hepatocytes
Differentiation

Teratoma Analysis ,
iPSC Characterization

Services to identify & characterize Pluripotent Stem Cells (iPSC/ ESC): Teratoma Analysis, Pluripotency Marker Assays, qPCR, RNA-seq & more.

Teratoma Analysis,
iPSC Characterization

iPSC Culture
Services

We offer standard and customized services for culture expansion, scale-up, and 3D culture morphology testing of iPSC and differentiated cells.

iPSC Culture
Services

Custom 3D iPSC Culture
Services - Organoid Modeling

Xeno-free, in vivo-like 3D culture of customer’s control/ patient iPSC lines using the MyEZGel™ nanopeptide, hydrogel-based matrix.

Custom 3D iPSC Culture
Services - Organoid Modeling

Stem Cell
Derivation

Mouse embryonic stem cell (ESC) derivation service from any mouse strain, including derivation of isogenic cell lines from transgenic mice.

Stem Cell
Derivation

Products and Services
Catalog ID#Product Name Price

34 Items

per page
FAQs
What are iPSCs?
What are stem cells?
What types of services do you offer in your stem cell platform?
Do you use feeder-dependent or feeder-free conditions?
Publications

iPSC Generation

  • Allende, M. L., Cook, E. K., Larman, B. C., Nugent, A., Brady, J. M., Golebiowski, D., ... & Proia, R. L. (2018). Cerebral organoids derived from Sandhoff disease induced pluripotent stem cells exhibit impaired neurodifferentiation. Journal of Lipid Research, jlr-M081323.
  • Field, A. R., Jacobs, F. M., Fiddes, I. T., Phillips, A. P., Reyes-Ortiz, A. M., LaMontagne, E., ... & Katzman, S. (2017). Structurally conserved primate lncRNAs are transiently expressed during human cortical differentiation and influence cell type specific genes. bioRxiv, 232553.

Safe Harbor Locus Master iPSC Generation with TARGATT™

  • Karow, M., Chavez, C. L., Farruggio, A. P., Geisinger, J. M., Keravala, A., Jung, W. E., ... & Calos, M. P. (2011). Site‐Specific Recombinase Strategy to Create Induced Pluripotent Stem Cells Efficiently with Plasmid DNA. Stem Cells, 29(11), 1696-1704.
  • Zhu, F., Gamboa, M., Farruggio, A. P., Hippenmeyer, S., Tasic, B., Schüle, B., ... & Calos, M. P. (2013). DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Nucleic acids research, 42(5), e34-e34.

iPSC Disease Modeling

Teratoma Analysis, iPSC Characterization

Teratoma Formation Assay (Published reports of ASC's Teratoma Formation Analysis Reports can be found in the Certificate of Analysis "Induced Pluripotent Stem Cells (iPSCs)" by Coriell Institute for Medical Research.)

References (*cited/published articles)

  • Ou, J., Ball, J. M., Luan, Y., Zhao, T., Miyagishima, K. J., Xu, Y., ... & Mallon, B. S. (2018). iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications. Cell, 173(4), 851-863. https://doi.org/10.1016/j.cell.2018.03.010

  • Teves, S. S., An, L., Bhargava-Shah, A., Xie, L., Darzacq, X., & Tjian, R. (2018). A stable mode of bookmarking by TBP recruits RNA Polymerase II to mitotic chromosomes. bioRxiv, 257451. DOI: 10.1101/257451

  • Hansen, A. S., Pustova, I., Cattoglio, C., Tjian, R., & Darzacq, X. (2017). CTCF and cohesin regulate chromatin loop stability with distinct dynamics. Elife, 6.

  • Vermilyea, S. C., Guthrie, S., Meyer, M., Smuga-Otto, K., Braun, K., Howden, S., ... & Golos, T. G. (2017). Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Adult Common Marmoset Fibroblasts. Stem cells and development, 26(17), 1225-1235. https://doi.org/10.1089/scd.2017.0069.

  • Teves, S. S., An, L., Hansen, A. S., Xie, L., Darzacq, X., & Tjian, R. (2016). A dynamic mode of mitotic bookmarking by transcription factors. Elife, 5.

  • Laskowski, T. J., Van Caeneghem, Y., Pourebrahim, R., Ma, C., Ni, Z., Garate, Z., ... & Segovia, J. C. (2016). Gene correction of iPSCs from a Wiskott-Aldrich syndrome patient normalizes the lymphoid developmental and functional defects. Stem cell reports, 7(2), 139-148.

  • Boza-Morán, M. G., Martínez-Hernández, R., Bernal, S., Wanisch, K., Also-Rallo, E., Le Heron, A., ... & Tizzano, E. F. (2015). Decay in survival motor neuron and plastin 3 levels during differentiation of iPSC-derived human motor neurons. Scientific reports, 5, 11696.

  • Romero, I. G., Pavlovic, B. J., Hernando-Herraez, I., Zhou, X., Ward, M. C., Banovich, N. E., ... & Chavarria, C. I. (2015). A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics. Elife, 4.

  • Cheung, H. S., Pelaez, D., & Huang, C. C. (2015). U.S. Patent Application No. 14/382,287.

  • Chakravarti, D., Su, X., Cho, M. S., Bui, N. H. B., Coarfa, C., Venkatanarayan, A., ... & Leung, M. L. (2014). Induced multipotency in adult keratinocytes through down-regulation of ΔNp63 or DGCR8. Proceedings of the National Academy of Sciences, 111(5), E572-E581.

  • Lee, J., Kim, Y., Yi, H., Diecke, S., Kim, J., Jung, H., ... & Park, S. H. (2014). Generation of disease-specific induced pluripotent stem cells from patients with rheumatoid arthritis and osteoarthritis. Arthritis research & therapy, 16(1), R41.

  • Quang, T., Marquez, M., Blanco, G., & Zhao, Y. (2014). Dosage and cell line dependent inhibitory effect of bFGF supplement in human pluripotent stem cell culture on inactivated human mesenchymal stem cells. PloS one, 9(1), e86031.

  • Jumabay, M., Abdmaulen, R., Ly, A., Cubberly, M. R., Shahmirian, L. J., Heydarkhan-Hagvall, S., ... & Boström, K. I. (2014). Pluripotent stem cells derived from mouse and human white mature adipocytes. Stem cells translational medicine, 3(2), 161-171.

  • Sanders, L. H., Laganière, J., Cooper, O., Mak, S. K., Vu, B. J., Huang, Y. A., ... & Langston, J. W. (2014). LRRK2 mutations cause mitochondrial DNA damage in iPSC-derived neural cells from Parkinson's disease patients: reversal by gene correction. Neurobiology of disease, 62, 381-386.

  • Sun, N., & Zhao, H. (2014). Seamless correction of the sickle cell disease mutation of the HBB gene in human induced pluripotent stem cells using TALENs. Biotechnology and bioengineering, 111(5), 1048-1053.

  • Lee, P., Martin, N. T., Nakamura, K., Azghadi, S., Amiri, M., Ben-David, U., ... & Lowry, W. E. (2013). SMRT compounds abrogate cellular phenotypes of ataxia telangiectasia in neural derivatives of patient-specific hiPSCs. Nature communications, 4, 1824.

  • Buccini, S. M. (2013). Doctoral dissertation, University of Cincinnati.

  • Pelaez, D., Huang, C. Y. C., & Cheung, H. S. (2013). Isolation of pluripotent neural crest-derived stem cells from adult human tissues by connexin-43 enrichment. Stem cells and development, 22(21), 2906-2914.

  • Cassidy, L., Choi, M., Meyer, J., Chang, R., & Seigel, G. M. (2013). Immunoreactivity of Pluripotent Markers SSEA-5 and L1CAM in Human Tumors, Teratomas, and Induced Pluripotent Stem Cells. Journal of Biomarkers, 2013, 960862. http://doi.org/10.1155/2013/960862.

  • Cooper, O., Seo, H., Andrabi, S., Guardia-Laguarta, C., Graziotto, J., Sundberg, M., … Isacson, O. (2012). Familial Parkinson’s disease iPSCs show cellular deficits in mitochondrial responses that can be pharmacologically rescued. Science Translational Medicine, 4(141), 141ra90. http://doi.org/10.1126/scitranslmed.3003985.

  • Zheng, Z., Jian, J., Zhang, X., Zara, J. N., Yin, W., Chiang, M., ... & Soo, C. (2012). Reprogramming of human fibroblasts into multipotent cells with a single ECM proteoglycan, fibromodulin. Biomaterials, 33(24), 5821-5831.

  • Almeida, S., Zhang, Z., Coppola, G., Mao, W., Futai, K., Karydas, A., ... & Sena-Esteves, M. (2012). Induced pluripotent stem cell models of progranulin-deficient frontotemporal dementia uncover specific reversible neuronal defects. Cell reports, 2(4), 789-798.

  • Zhang, W. Y., de Almeida, P. E., & Wu, J. C. (2012). Teratoma formation: A tool for monitoring pluripotency in stem cell research. StemBook.

  • Jing, L., Christoforou, N., Leong, K. W., Setton, L. A., & Chen, J. (2012). Differentiation potential of human induced pluripotent stem cells (iPSCs) to nucleus pulposus-like cells in vitro. Global Spine Journal, 2(1_suppl), s-0032.

  • Valamehr, B., Abujarour, R., Robinson, M., Le, T., Robbins, D., Shoemaker, D., & Flynn, P. (2012). A novel platform to enable the high-throughput derivation and characterization of feeder-free human iPSCs. Scientific reports, 2, 213.

  • Chen, K. G., Mallon, B. S., Hamilton, R. S., Kozhich, O. A., Park, K., Hoeppner, D. J., ... & McKay, R. D. (2012). Non-colony type monolayer culture of human embryonic stem cells. Stem cell research, 9(3), 237-248.

  • Telugu, B. P. V. L., Ezashi, T., Sinha, S., Alexenko, A. P., Spate, L., Prather, R. S., & Roberts, R. M. (2011). Leukemia Inhibitory Factor (LIF)-dependent, Pluripotent Stem Cells Established from Inner Cell Mass of Porcine Embryos. The Journal of Biological Chemistry, 286(33), 28948–28953. http://doi.org/10.1074/jbc.M111.229468.

  • Deleidi, M., Hargus, G., Hallett, P., Osborn, T., & Isacson, O. (2011). Development of histocompatible primate induced pluripotent stem cells for neural transplantation. Stem Cells (Dayton, Ohio), 29(7), 1052–1063. http://doi.org/10.1002/stem.662

Karyotyping (*cited/published articles)

  • Zhao, L., Teklemariam, T., & Hantash, B. M. (2014). Heterelogous expression of mutated HLA-G decreases immunogenicity of human embryonic stem cells and their epidermal derivatives. Stem cell research, 13(2), 342-354.
  • Sun, N., & Zhao, H. (2014). Seamless correction of the sickle cell disease mutation of the HBB gene in human induced pluripotent stem cells using TALENs. Biotechnology and bioengineering, 111(5), 1048-1053.
  • An, M. C., Zhang, N., Scott, G., Montoro, D., Wittkop, T., Mooney, S., ... & Ellerby, L. M. (2012). Genetic correction of Huntington's disease phenotypes in induced pluripotent stem cells. Cell stem cell, 11(2), 253-263.
  • Zheng, Z., Jian, J., Zhang, X., Zara, J. N., Yin, W., Chiang, M., ... & Soo, C. (2012). Reprogramming of human fibroblasts into multipotent cells with a single ECM proteoglycan, fibromodulin. Biomaterials, 33(24), 5821-5831.
Have Questions?

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