Humanized Strategy
Validation Data
Figure 1. Detection of IL2 expression by RT-PCR.
Wild type: only one band at 255 bp with primers F2/R2 (mIl2); Homozygous: only one band at 320 bp with primers F1/R1 (hIL2). Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Figure 2. Expression of hIL2 in the Serum of heterozygous humanized IL2mice was detected by ELISA.
Figure 3. Expression of mIl2 in the serum of heterozygous humanized IL2 mice was detected by ELISA.
Figure 4. Splenocytes from humanized IL2 mice were analyzed by flow cytometry after activation.
The hIL2+ (but not mIl2+) cells were detected in the homozygous hIL2 mice.