Customized Mouse Models using CRISPR/Cas9
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Applied StemCell’s PrimeCKO™ Conditional Knockout Mouse Models service will enable you to have precise control over where or when your gene of interest in knocked out. Using CRISPR and the Cre-Lox system, we can generate floxed Conditional Knock-out Mouse Models for a variety of temporal and spatial knock-out conditions in mice.
- Tissue-specific/ inducible knockout; also suitable for constitutive knockout
- F1 breeding for germline transmission
- Fast turnaround of 5-8 months
- Novel CRISPR strategy for higher efficiency and success rate
- Animal generated in a AAALAC-accredited animal facility in the USA
- Animal IP belongs to customers
You can breed the “floxed” PrimeCKO™ conditional knockout mice with commercially available Cre mouse lines or we can custom generate a Cre mouse model using our TARGATT™ site-specific knock-in technology for high level Cre expression.
- CRISPR Vector construction: gRNA design, validation, donor DNA construction, in vitro transcription and QC for Microinjection
- Pronuclear injection and embryo transfer
- Genotyping of offspring for integration, animal care and housing
- Recommended: F1 Breeding of founders for germline transmission
Timeline: Founders (F0), 3-6 months; Germline transmitted F1, 5-8 months
- At least 2 founders (or germline transmitted F1s) with confirmed mutation
- Milestone update/report; final report with detailed description of each procedure, including targeting vector design, construction and validation, transfection condition, genotyping strategy, and results.
- Study critical gene/ protein mechanism and function at the tissue-specific/cellular level
- Disease modeling for gene dysfunction in specific cell types
- Cell-type specific target validation
- Preclinical safety and toxicity studies
For constitutive conditional knockout, ASC offers custom gene knockout mouse models using CRISPR/Cas9.
All our animals are housed in a facility that is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International, has an Assurance on file with the Office of Laboratory Animal Welfare (OLAW), and is a registered research facility with the U.S. Department of Agriculture.