The endogenous mice F12 gene was replaced by human F12 gene.
Validation Data
Figure 1. Detection of F12 expression in liver by RT-PCR.
Wild type: only one band at 318 bp with primers F1/R1 (mF12); Homozygous: only one band at 274 bp with primers F2/R2 (hF12); Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Figure 2. Detection of hF12 expression in serum by ELISA (n=2).
Abbr. HO, homozygous; HE, heterozygous; WT, wild type.
Figure 3. Detection of mF12 expression in serum by ELISA (n=2).
Abbr. HO, homozygous; HE, heterozygous; WT, wild type.