The endogenous mice Gdf15 gene was replaced by human GDF15 gene.
Validation Data
Figure 1. Detection of GDF15 expression in intestine and colon and liver by RT-PCR.
Wild type: only one band at 314 bp with primers F1/R1 (mGdf15); Homozygous: only one band at 160 bp with primers F2/R2 (hGDF15); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Figure 2. Detection of hGDF15 expression in plasma by ELISA.
Abbr. HO, homozygous; HE, heterozygous; WT, wild type.
Figure 3. Detection of mGDF15 expression in plasma by ELISA.
Abbr. HO, homozygous; HE, heterozygous; WT, wild type.