The endogenous mice Gcgr gene was replaced by human GCGR gene.
Validation Data
Figure 1. Detection of GCGR expression in liver by RT-PCR.
Wild type: only one band at 261 bp with primers F1/R1 (mGcgr); Heterozygous: one band at 261 bp with primers F1/R1 (mGcgr) and one band at 223 bp with primers F2/R2 (hGCGR); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Figure 2. Detection of human GCGR expression in liver in hGCGR knockin mice by IHC.
Liver were collected from wild-type mice (+/+) and homozygous hGCGR mice (HO/HO), and analyzed by IHC with anti-human GCGR antibody.