Trem2-CKO - Other Gene Edited Mouse Models (KO, CKO) - Mouse Repository - Animal Models - Research

SKU :
ASCKO-190063
Catalog # :
ASCKO-190063
Availability :
Mouse
Humanized Gene Knock-in Strategy Figure 1. Targeting strategy for Trem2 gene CKO mouse model by ES cell targeting method. Genotyping Strategy Figure 2. Genotyping strategy for heterozygous F1 mice. Genotyping PCR was done using primer sets for the 5’ arm (regions 1 and 2) and 3’ arm (regions 3 and 4). The 5’ arm PCR yielded a 6.4 kb amplified fragment in the positive F1 mice and a 10.9 kb fragment would be amplified. The 3’ arm PCR yielded a 7.1 kb fragment in F1 p .....
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Strain Information
  • Catalog Number ASCKO-190063
  • Model Name Trem2-CKO
  • Strain Name C57BL/6J-Trem2em2(flox)Asc
  • GENE Synonyms Trem2a, Trem2b, Trem2c
  • Human Orthologs TREM2
  • Emsamble ID ENSMUSG00000023992
  • MGI ID 1913150
  • NCBI ID 83433
  • Backgound C57BL/6J
  • Coat Color Black
Description

Humanized Gene Knock-in Strategy

Landingpage-ASCKO-190063-TREM2-CKO-Mouse-1

Figure 1. Targeting strategy for Trem2 gene CKO mouse model by ES cell targeting method.

Genotyping Strategy

Landingpage-ASCKO-190063-TREM2-CKO-Mouse-2

Figure 2. Genotyping strategy for heterozygous F1 mice. Genotyping PCR was done using primer sets for the 5’ arm (regions 1 and 2) and 3’ arm (regions 3 and 4). The 5’ arm PCR yielded a 6.4 kb amplified fragment in the positive F1 mice and a 10.9 kb fragment would be amplified. The 3’ arm PCR yielded a 7.1 kb fragment in F1 positive mice and a 11.1 kb fragment in negative F1 mice.

PCR Gel Electrophoresis to Genotype Recombinant F1 Mice

Landingpage-ASCKO-190063-TREM2-CKO-Mouse-3

Figure 3. Agarose gel electrophoresis of PCR results. 5’ recombinant fragment (5'arm ), 3’ recombinant fragment (3'arm ). Six positive F1 mice were identified and confirmed by Sanger sequencing (data not shown). WT wild type control; M: 1kb DNA ladder.

Research Application
Immunotherapy
Availability
Mouse
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