Exons 1-7 were replaced by CreERT2 and a loxp neo cassette. The KI mice have a tamoxifen-inducible cre-mediated recombination system.
Validation Data
Figure 1. X-gal staining of liver slides of Alb-CreERT2 and LacZ positive mouse before and after tamoxifen injection.
Figure 2: Immunofluorescence analysis of liver using anti-Alb (Green) and anti-LacZ (Red) antibodies showed that Cre is expressed in most hepatocytes.
Figure 3: Blood biochemical assay of Alb-CreERT2 male and female mice. Alb-CreERT2 mice do not display any liver functional abnormalities.