Cd8a-(V5-IRES-Akaluc-2A-EGFP)

Using CRISPR gene editing technology, a V5-IRES-Akaluc-2A-EGFP expression cassette was inserted before the stop codon TAA of mouse Cd8a gene to establish a multi-labeled Knock-in model with V5, Akaluc luciferase and EGFP fluorescent protein. Akaluc is a novel luciferase that produces up to 1,000 times as bright as light emitted by existing systems by feeding or injecting a synthetic luciferin compound called AkaLumine-HCl, which is better able to penetrate the blood-brain barrier than d-luciferi .....