A CAG-LSL-mTagBFP2-3xFLAG-WPRE-polyA cassette was inserted at the mouse H11 locus using CRISPR gene editing technology. When bred to a strain expressing Cre recombinase under control of various tissue specific promoters, Cre recombination deletes the LSL cassette and allows the expression of the mTagBFP2-3xFLAG protein.
Figure 1. BFP expression in CAG-mTagBFP2/+ mouse, visualized using fluorescence imaging.
CAG-mTagBFP2/+ mouse was achieved through crossing CAG-LSL-mTagBFP2/+ mouse and Cre-expressing mouse. BFP expression in different organs of CAG-mTagBFP2/+ mouse was detected using fluorescence imaging, which suggests relatively high expression of BFP in a variety of organs. A: Images of different organs of CAG-mTagBFP2/+ mouse; B: Images of histologic sections of CAG-mTagBFP2/+ mouse under confocal microscopy.