A CAG-LSL-mTagBFP2-3xFLAG-WPRE-polyA cassette was inserted at the mouse H11 locus using CRISPR gene editing technology. When bred to a strain expressing Cre recombinase under control of various tissue specific promoters, Cre recombination deletes the LSL cassette and allows the expression of the mTagBFP2-3xFLAG protein.
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