These mice harbor a CAG-EGFP-WPRE-polyA cassette in the Rosa26 locus.2 generated by homologous recombination.
Validation Data
Figure 1: Detection of EGFP (Green) in various tissues of R26-CAG-EGFP(3) mice. Expression tissues include: brain, eyes, heart, lungs, liver, stomach, salivary glands, adrenal glands, kidneys, colon, small intestine, spleen, pancreas, uterus and ovaries, skin, thymus, pituitary gland. A few cells expressed in spinal cord and brown fat. (37-week old and 10.3-week old, female)
Figure 2: Comparison of EGFP expression in live CD45 blood cells and live cells isolated from various organs.
Figure 3: Comparison of EGFP expression in live CD45 cells in PB, bone marrow, spleen and thymus.