TARGATT™ Master Cell Line Generation Service Details
- Standard
- Fully Customizable Service - Inquire
Standard Packaging:
- Ship 2 million frozen cells in cryovials (two (2) vials with 1 x 10^6 cells/vial)
- With pathogen and SARS-CoV-2 test results
- Optional Pathogen Testing Service is available
- With pathogen and SARS-CoV-2 test results
Standard Deliverables:
- Two (2) iPSC clones with two (2) vials per clone at 1 x 10^6 cells/ vial
- Clones are characterized for three (3) pluripotency markers: OCT4, SOX2, SSEA4, TRA-1-60, TRA-1-81
- Karyotyping Data
- Additional characterization services are available.
- Final Report
Standard Workflow and Timeline:
- Timeline: 3-5 months
TARGATT™ Overview
Applied Stem Cell Inc.’s proprietary TARGATT™ technology enables highly efficient site-specific gene insertion in mammalian cells and animals. This technology uses ФC31 integrase to insert any gene of interest into a docking site that has been pre-engineered in an intergenic and transcriptionally active genomic locus. Our TARGATT™ technology improves several aspects in the generation of transgenic cell lines and animals:

How does it work?
- You can provide us with mycoplasma test results, or we can do the mycoplasma testing for you
- Some cancer cells may require karyotype results
- You can specify where you would like the attP sites to be located, or we can recommend genomic locations
Adding TARGATT sites to your cell line allows you to insert any gene of interest in a specific locus in the genome.
Related Service
TARGATT™ Knock-in Cell Line Generation: Integration of your gene of interest into our TARGATT™ Master Cell Line which contains a pre-engineered "attP" landing pad.
- Turnaround time: 3-5 months