TARGATT™ Genome Editing
TARGATT™ site-specific knock-in technology enables the integration of transgenes at a preselected locus in the genome, thus overcoming drawbacks of random integration. This technology is adaptable for gene integration in many different species of animals (mice, rats, rabbits, pigs) and cell lines (including CHO cells, stem cells and immortalized cell lines).
Applications:
- Transgene overexpression models
- Humanized animal models
- Reporter gene insertion models
- Inducible expression models
- Cre - driver lines
TARGATT™ Genome Editing Categories
TARGATT™ Knockin
Master Cell Line
Products and Services
Supporting Material
Brochures/ Flyers:
Posters:
Webinars:
How-to Guide for TARGATT™ Transgenic Kit: Dr Ruby Chen-Tsai- Choosing the Right Genome Editing Technology for Your Mouse Models: CRISPR, TARGATT™ and Beyond...: Dr. Carlisle Landel
- TARGATT™: A Mammalian System for Antibody/ Protein Library Screening (October 2018)
Technical Details
Advantages of TARGATT™ Technology:
- High integration efficiency (up to 40%)
- Large transgene knock-in (up to 22 kb)
- Reduced time and cost
- Guaranteed, high level expression of the transgene
- Site-specificity allows a precise comparison of the effects of the transgenes among different lines
- Site-specific knock-in at pre-selected locus overcomes challenges associates with random integration:
- Eliminates position effect
- Integration at intergenic region ensures that no internal genes are interrupted
- Single copy gene integration eliminates repeat-induced gene silencing and genomic instability
Choosing the right genome editing technology: Applied StemCell uses two complementary genome editing technologies to generate advanced cell line and animal models very efficiently and effectively: the CRISPR/Cas9 technology and our propriety site-specific gene integration technology, TARGATT™ for large fragment (up to 20 kb) knock-in into a safe harbor locus.
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Project Purpose |
CRISPR/Cas9 |
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Knock-Out (KO) |
Yes |
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Point Mutation |
Yes |
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Conditional KO |
Yes |
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Knock-In (<200 Nucleotide ssODN Donor) |
Yes |
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Knock-In Transgenes in Safe Harbor Loci (>2kb) |
Challenging (but limitations on size) |
Yes (up to 20kb) |
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Knock-In (Plasmid DNA) |
Challenging (but limitations on size) |
Yes (2 steps: KI docking site; KI transgene) |
