• TARGATT™ Genome Editing

TARGATT™ Genome Editing

TARGATT™ site-specific knock-in technology enables the integration of transgenes at a preselected locus in the genome, thus overcoming drawbacks of random integration. This technology is adaptable for gene integration in many different species of animals (mice, rats, rabbits, pigs) and cell lines (including CHO cells, stem cells and immortalized cell lines).

Applications:

  • Transgene overexpression models
  • Humanized animal models
  • Reporter gene insertion models
  • Inducible expression models
  • Cre - driver lines
TARGATT™ Genome Editing Categories

TARGATT™ Mouse Model Generation

Do-it-yourself kits to generate site-specific transgenic mice in 3 months! We offer TARGATT™ “attP” mice, plasmids, transgenic kits, genotyping kits.

TARGATT™ Mouse Model Generation

TARGATT™ Knockin
Master Cell Line

TARGATT™ “attP” Master cell lines and knock-in kit for rapid, site-specific transgene knock-in with high efficiency and robust transgene expression.

TARGATT™ Knockin
Master Cell Line

Products and Services
Catalog ID#Product Name SizePriceQTY
$199.00
$1,250.00
$57.20
$199.00
$2,500.00
$59.55
$59.55
$550.00
$1,300.00
$675.00
$550.00
$550.00
$550.00
$550.00
$550.00

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Technical Details

Advantages of TARGATT™ Technology:

  • High integration efficiency (up to 40%)
  • Large transgene knock-in (up to 22 kb)
  • Reduced time and cost
  • Guaranteed, high level expression of the transgene
  • Site-specificity allows a precise comparison of the effects of the transgenes among different lines
  • Site-specific knock-in at pre-selected locus overcomes challenges associates with random integration:
  • Eliminates position effect
  • Integration at intergenic region ensures that no internal genes are interrupted
  • Single copy gene integration eliminates repeat-induced gene silencing and genomic instability


Choosing the right genome editing technology: Applied StemCell uses two complementary genome editing technologies to generate advanced cell line and animal models very efficiently and effectively: the CRISPR/Cas9 technology and our propriety site-specific gene integration technology, TARGATT™ for large fragment (up to 20 kb) knock-in into a safe harbor locus.

Project Purpose

CRISPR/Cas9

TARGATT™

Knock-Out (KO)

Yes

 

Point Mutation

Yes

 

Conditional KO

Yes

 

Knock-In

(<200 Nucleotide ssODN Donor)

Yes

 

Knock-In Transgenes in

Safe Harbor Loci (>2kb)

Challenging

(but limitations on size)

Yes

 (up to 20kb)

Knock-In

 (Plasmid DNA)

Challenging

(but limitations on size)

Yes

 (2 steps: KI docking site; KI transgene) 

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!