CRISPR Custom Point Mutation Cell Line Generation Service

Generate physiologically relevant in vitro model with mutations seen in clinical specimens in your preferred cell lines. ASC can knock-in or correct point mutations in your gene of interest in control or disease cell lines. Very ideal for immune-oncology research and drug discovery, our CRISPR/Cas9 cell line model generation protocols can generate precise point mutations in the targeted locus.

• With or without silent mutation; footprint-free genome editing capable
• Homozygous/ heterozygous variant clones
• Variety of cell lines: cancer, hard-to-transfect, most mammalian species

Workflow includes:

• gRNA design, construction, and validation
• Donor DNA construction
• Cell line validation, transfection and optimization
• Screening for single cell clones and clone confirmation
• Cell expansion and cryopreservation

Deliverables:

• Two homozygous clones with correct gene mutation for the modified line; two vials of each clone; 1 x 10^6 cells/vial.
• Milestone update/report; final report with detailed description of each procedure, including targeting vector design, construction and validation, transfection condition, genotyping strategy, and results.

Timeline: 3-4 months

Applications:

• Disease modeling for immuno-oncology, pharmacogenomic studies
• Drug discovery and drug efficacy and toxicity screening; drug combination studies
• Deriving diagnostic reference standards and materials