Cell Line Immortalization Service

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Simian virus 40 (SV40) T antigen has been shown to be the simplest and most reliable agent for the immortalization of many different cell types and the mechanism of SV40 T antigen in cell immortalization is relatively well understood. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells. 

Service includes:

- Lentivirus vector(s) expressing SV40 large and small T antigens
- Transduction, colony selection (up to 10 passages)
- Characterization (transgene expression by RT-PCR)
- Culture, cryopreservation

Required Materials: Customer-desired cells, 2 vials of typical storage size, and indication of appropriate growth medium and conditions. If your cells require medium other than DMEM and RPMI, we will need 1-2 L of medium and any applicable growth factors from you for the project. Also, please provide coated 6-well plates and T25 flasks if the cells require specially treated culture vessels.

Deliverables: Applied StemCell, Inc. will provide 2 vials (~1 x 10^6 cells/vial) of immortalized cells.

Timeline: 2-5 months depending on immortalizing method, source cells, and species

Standard service fee for following samples:

  • Primary Fibroblasts (from healthy or disease donor)
  • Smooth Muscle Cells
  • MEF cells

Please ask about restrictions or possible experimental services for the following samples or others not listed:

  • Leukemia cells
  • B cells
  • Lymphoblastoid cells
  • Peripheral blood mononuclear cells (PBMC)
  • T cells
  • Macrophages
  • Retinal ganglion cells
  • Tumor cells
  • Chondrocytes
  • Hepatocytes
  • Myoblasts
  • Osteoblasts
  • Primary cell from ovary cancer tissue

Currently we are not accepting following samples:

  • Neurons
  • Dendritic cells



Some primary cells may encounter what’s known as a “crisis stage” after over ~25-30 passages. Therefore, truly immortalized cell lines must be cultured long-term to pass the “crisis stage.” Our guarantee includes the positive transduction and expression of T antigen in target cells. If required, ASC will add the long-term culture to test beyond the “crisis stage” option for additional charge, which will be estimated based on the growth properties of the transduced cells; please contact us for an additional quote.