Lentivirus Stable Cell Line Generation - Lentivirus Stable Cell Line Generation - Services - Research

SKU :
ASC-7104
Catalog # :
ASC-7104
A comprehensive custom Lentiviral-based Stable Cell Line Generation Service for genetically engineering sensitive cells, primary cells, stem cells and terminally differentiated cells. Lentivirus-based stable cell line generation offers a cost-effective and efficient alternative for generating stable cell line models for gene overexpression, inducible gene expression, gene knockout and gene knockdown. Broad tropism lentiviruses to target most mammalian cell types Non-integrating lentiviruses .....
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Description

A comprehensive custom Lentiviral-based Stable Cell Line Generation Service for genetically engineering sensitive cells, primary cells, stem cells and terminally differentiated cells. Lentivirus-based stable cell line generation offers a cost-effective and efficient alternative for generating stable cell line models for gene overexpression, inducible gene expression, gene knockout and gene knockdown.

  • Broad tropism lentiviruses to target most mammalian cell types
  • Non-integrating lentiviruses for CRISPR-lenti gene knockout
  • Choice of selection markers: antibiotic resistance or GFP
  • 3rd generation lentiviral systems for the highest biosafety standards
  • Gene editing confirmed by NGS or RT-PCR

Cell Line Modifications:

  • Knock-in Cell Line Generation: We offer 3rd generation lentiviral system for stable gene knock-in into multiple cell types from most mammalian species.
  • CRISPR Knockout Cell Line Generation: We offer non-integrating lentiviruses (IDLVs) for CRISPR-lenti gene knockout or knock down modifications in hard-to-transfect cell lines.

Workflow includes:

  • Cell line validation
  • Generation of lentivirus; CRISPR vector construction and lentivirus packaging (if needed)
  • Reagent validation
  • Lentivirus transduction
  • Clone screening and confirmation
  • Cell expansion and cryopreservation

Service Specifications:

Customer should provide:

  • Frozen cells: 2 vials of 10^6 cells/ vial OR live cells: 2 x T25 flasks at 90% confluency
  • Detailed information regarding target gene and gene modification

Deliverables:

  • Two vials of 1-2 clones or two vials of pooled clone.
  • Optional: negative control non-target gRNA-virus available
  • Milestone update/report; final report with detailed description of each procedure, including targeting vector design, construction and validation, transfection condition, genotyping strategy, and results.

Timeline: 3-6 months

Applications:

  • Drug screening
  • Functional assays
  • Membrane protein screening
  • Long-term expression studies
  • Antibody immunization boosting
  • Gene therapy research 
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