The endogenous mice Il18 gene was replaced by human IL18 gene.
Validation Data
Figure1. Detection of IL18 expression in intestine, colon and spleen by RT-PCR.
Wild type: only one band at 305 bp with primers F1/R1 (mIl18); Homozygous: only one band at 265 bp with primers F2/R2 (hIL18). Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Figure 2. Detection of hIL18 expression in serum by ELISA (n=3).
Abbr. HO, homozygous; HE, heterozygous; WT, wild type.
Figure 3. Detection of mIL18 expression in serum by ELISA (n=3).
Abbr. HO, homozygous; HE, heterozygous; WT, wild type.