CRISPR Cre-Rat with Pomc-CreERT2 Promoter (Embryo) - Cre Rat Models - Rat Models - Animal Models - Research

SKU :
553-1
Catalog # :
553-1
MTA required. The Pomc-CreERT2 rat model was generated using CRISPR/Cas9 for use as a breeding mate for conditional knockout/ knock-in rat models. To achieve this, a pair of in vivo validated gRNAs, a single-stranded deoxyribonucleic acid (ssDNA) donor, and the Cas9 protein were injected into the mouse embryos.  Mice born from the injection were screened by genotyping PCR and NGS/ Sanger sequencing to confirm founders (F0). The Cre rat line generated expresses Cre recombinase under the cont .....
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Description

MTA required. The Pomc-CreERT2 rat model was generated using CRISPR/Cas9 for use as a breeding mate for conditional knockout/ knock-in rat models. To achieve this, a pair of in vivo validated gRNAs, a single-stranded deoxyribonucleic acid (ssDNA) donor, and the Cas9 protein were injected into the mouse embryos.  Mice born from the injection were screened by genotyping PCR and NGS/ Sanger sequencing to confirm founders (F0). The Cre rat line generated expresses Cre recombinase under the control of tissue-specific gene promoter Pomc.  Furthermore, DNA recombinase activity is induced by Tamoxifen, so that the Cre-Lox model can be both conditional and inducible.

Tissue/Cell-specificity

Rat line

Promoter

Reference

Neurons involved in the control of food intake (arcuate nucleus (hypothalamus) and solitary tract nucleus (hindbrain))

Pomc-CreERT2

13-kb of 5′ and 2 kb of 3′ mouse Pomc flanking sequences

 

Padilla et al 2011

Young et al 1998

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