CRISPR Cre-Rat with Pomc-CreERT2 Promoter (Embryo)

Reference the resources below for more information about the product/service or contact us today to learn more.
Catalog # :
  • Description
  • Related Products

MTA required. The Pomc-CreERT2 rat model was generated using CRISPR/Cas9 for use as a breeding mate for conditional knockout/ knock-in rat models. To achieve this, a pair of in vivo validated gRNAs, a single-stranded deoxyribonucleic acid (ssDNA) donor, and the Cas9 protein were injected into the mouse embryos.  Mice born from the injection were screened by genotyping PCR and NGS/ Sanger sequencing to confirm founders (F0). The Cre rat line generated expresses Cre recombinase under the control of tissue-specific gene promoter Pomc.  Furthermore, DNA recombinase activity is induced by Tamoxifen, so that the Cre-Lox model can be both conditional and inducible.


Rat line



Neurons involved in the control of food intake (arcuate nucleus (hypothalamus) and solitary tract nucleus (hindbrain))


13-kb of 5′ and 2 kb of 3′ mouse Pomc flanking sequences


Padilla et al 2011

Young et al 1998