CRISPR Cre-Rat with Wnt1-CreERT2 Promoter (Embryo)

MTA required. This conditional knock-in Cre rat model uses CRISPR/Cas9-mediated cleavage and subsequent homology-directed repair (HDR) by flanking the Wnt1-CreERT2 construct with two LoxP sequences. To achieve this, a pair of in vivo validated gRNAs, a single-stranded deoxyribonucleic acid (ssDNA) donor, and the Cas9 protein were injected into the mouse embryos.  Mice born from the injection were screened by genotyping PCR and NGS/ Sanger sequencing to confirm founders (F0). The Cre rat line generated expresses Cre recombinase under the control of tissue-specific gene promoter Wnt1.  Furthermore, DNA recombinase activity is induced by Tamoxifen, so that the Cre-Lox model will be conditional and inducible.