CRISPR Cre-Rat with Wnt1-CreERT2 Promoter (Rat)

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MTA Required. This conditional knock-in Cre rat model uses CRISPR/Cas9-mediated cleavage and subsequent homology-directed repair (HDR) by flanking the Wnt1-CreERT2 construct with two LoxP sequences. To achieve this, a pair of in vivo validated gRNAs, a single-stranded deoxyribonucleic acid (ssDNA) donor, and the Cas9 protein were injected into the mouse embryos.  Mice born from the injection were screened by genotyping PCR and NGS/ Sanger sequencing to confirm founders (F0). The Cre rat line generated expresses Cre recombinase under the control of tissue-specific gene promoter Wnt1.  Furthermore, DNA recombinase activity is induced by Tamoxifen, so that the Cre-Lox model will be conditional and inducible.


Rat line



Developing neural crest and midbrain


1.3-kb 5' promoter and 5.5 kb 3' enhancer of the mouse Wnt1, wingless-related MMTV integration site 1

Chou et al 2013