CRISPR Cre-Rat with HB9-CreERT2 Promoter (Embryo) - Cre Rat Models - Rat Models - Animal Models - Research

SKU :
554-1
Catalog # :
554-1
MTA required. The HB9-CreERT2 rat model was generated using CRISPR/Cas9 for use as a breeding mate for conditional knockout/ knock-in rat models. To achieve this, a pair of in vivo validated gRNAs, a single-stranded deoxyribonucleic acid (ssDNA) donor, and the Cas9 protein were injected into the mouse embryos.  Mice born from the injection were screened by genotyping PCR and NGS/ Sanger sequencing to confirm founders (F0). The Cre rat line generated expresses Cre recombinase under the contr .....
Inquire
Description

MTA required. The HB9-CreERT2 rat model was generated using CRISPR/Cas9 for use as a breeding mate for conditional knockout/ knock-in rat models. To achieve this, a pair of in vivo validated gRNAs, a single-stranded deoxyribonucleic acid (ssDNA) donor, and the Cas9 protein were injected into the mouse embryos.  Mice born from the injection were screened by genotyping PCR and NGS/ Sanger sequencing to confirm founders (F0). The Cre rat line generated expresses Cre recombinase under the control of tissue-specific gene promoter HB9.  Furthermore, DNA recombinase activity is induced by Tamoxifen, so that the Cre-Lox model can be both conditional and inducible.

Tissue/Cell-specificity

Rat line

Promoter

Reference

Motor neurons

HB9-CreERT2

9-kb HB9 promoter

Arber et al 1999 Yang et al 2001; Nakano et al 2005; Tasic et al 2011

Google