CRISPR Cre-Rat with Tie2-CreERT2 Promoter (Rat) - Cre Rat Models - Rat Models - Animal Models - Research

SKU :
559-2
Catalog # :
559-2
MTA required. The Tie2-CreERT2 rat model was generated using CRISPR/Cas9 for use as a breeding mate for conditional knockout/ knock-in rat models. To achieve this, a pair of in vivo validated gRNAs, a single-stranded deoxyribonucleic acid (ssDNA) donor, and the Cas9 protein were injected into the mouse embryos.  Mice born from the injection were screened by genotyping PCR and NGS/ Sanger sequencing to confirm founders (F0). The Cre rat line generated expresses Cre recombinase under the cont .....
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Description

MTA required. The Tie2-CreERT2 rat model was generated using CRISPR/Cas9 for use as a breeding mate for conditional knockout/ knock-in rat models. To achieve this, a pair of in vivo validated gRNAs, a single-stranded deoxyribonucleic acid (ssDNA) donor, and the Cas9 protein were injected into the mouse embryos.  Mice born from the injection were screened by genotyping PCR and NGS/ Sanger sequencing to confirm founders (F0). The Cre rat line generated expresses Cre recombinase under the control of tissue-specific gene promoter Tie2.  Furthermore, DNA recombinase activity is induced by Tamoxifen, so that the Cre-Lox model can be both conditional and inducible.

Tissue/Cell-specificity

Rat line

Promoter

Reference

Vascular endothelial cells including brain and retinal capillary

Tie2-CreERT2

2.1-kb promoter +1.7kb intron 1 enhancer of mouse Tie2 (vascular endothelial-specific receptor tyrosine kinase)

Schlaeger et al 1997

Ohtsuki et al 2005

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